OptiPrep™ [Catalog No.: AXS-1114542, AXS-1114542-5]

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Product Description



OptiPrep™ is a sterile and endotoxin tested multi-purpose medium based on iodixanol. Unlike any of these other gradient media, iodixanol (as an X-ray imaging agent) has been clinically tested and is manufactured under strict and EU cGMP compliance.

OptiPrep™ specifications:

  • Iodixanol: 60% (w/v)
  • Density: 1.320 ± 0.001 g/ml
  • Osmolality: approx. 170 mOsm
  • Endotoxins: < 1.0 EU/ml

OptiPrep™ (iodixanol) is the ideal medium for:

  • Mammalian and nonmammalian cells
  • Purification of non-human lymphocytes gives maximum yield with no contamination of neutrophils>
  • Subcellular organelles
  • Analysis of membrane trafficking and cell signalling
  • Analysis of endocytosis and exocytosis
  • Plasma membrane and domain
  • Membrane vesicles and cytosol
  • Organelles from nonmammalian sources
  • Viruses
  • Plasma lipoproteins
  • Proteins and protein complexes
  • Plasmid DNA
  • Ribonucleoprotein

Product Downloads:

Optiprep™/ Nycodenz® Application Sheets

Warranty Information

More About OptiPrep™

  • Iodixanol was developed as an X-ray contrast medium and has therefore been subjected to rigorous clinical testing.
  • Iodixanol is non-ionic, non-toxic to cells and metabolically inert.
  • Iodixanol is approximately a dimer of Nycodenz® and its biological and physicochemical properties are very similar. However, iodixanol solutions have osmolalities approximately half that of Nycodenz® solutions of the same density. Iodixanol solutions can be made isoosmotic at all densities and OptiPrep™ (iodixanol) can be added directly to a cell or organelle suspension without changing its osmolality, thus facilitating their purification in a flotation gradient.
  • Because of its higher molecular mass, iodixanol forms self-generated gradients more quickly than Nycodenz®. Mammalian and non-mammalian cells purified in iodixanol gradients have high viability.
  • Organelles and membrane vesicles are effectively fractionated in either pre-formed or self-generated gradients.
  • Viruses purified in iodixanol gradients show an infectivity: particle ratio at least 100x higher than those from CsCl gradients.
  • Protein-protein and protein-nucleic acid complexes are better preserved in iodixanol than in sucrose or glycerol gradients.
  • Plasma lipoproteins analyzed in self-generated gradients within 4h.
  • Each batch of OptiPrep™ (iodixanol) is checked for endotoxin by the limulus amebocyte lysate (LAL) test. Our goal is to produce batches with an endotoxin level lower or equal to 0.13 EU/ml. For every batch produced a Certificate of Analysis showing the actual values of density and endotoxin levels is made available at www.axis-shield-density-gradientmedia.com.
  • OptiPrep™ (iodixanol) sterility is claimed according to Ph.Eur. OptiPrep™ is manufactured, packed and released in compliance with: 
    1. Current EU Guide to Good Manufacturing Practice 
    2. Fresenius Kabi AS Quality System 
    3. Fresenius Kabi AS Manufacturing Licence