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Serumwerk Density Gradient Media General Instructions, Application Sheets, and Literature References
   

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Cell Applications Sheets
(Sheet IDs begin with "C")
   Macromolecules Application Sheets
(Sheet IDs begin with "M")		  
Subcellular Applications  (S)
(Sheet IDs begin with "S")
  
 Virus Applications (V)
(Sheet IDs begin with "V")
  Index to Macromolecules Application Sheets
(A-Z by macromolecule class)
  
Index To Subcellular Components Application Sheets
(A-Z by organelle or membrane type grouped by mamallian and non-mammalian)
   Index to Virus Application Sheets
(A-Z by virus name within virus group type)
     
C02 – Preparation of gradients for cells   M02 – Preparation of discontinuous and continuous gradients (for macromolecules and macromolecular complexes)   S02 – Preparation of gradient solutions (non-mammalian)   V02 – Preparation of discontinuous and continuous gradients
C03 – Mononuclear cells, lymphocytes, monocytes and polymorphonuclear leukocytes from blood: a methodological review   M03 – Self-generated gradients (macromolecules/macromolecular complexes)   S03 – Preparation of discontinuous and continuous gradients   V03 – Preparation of self-generating gradients
C04 – Isolation of mononuclear cells from human blood by sedimentation on to a density barrier   M04 – Harvesting gradients   S04 – Preparation of self-generated gradients   V04 – Harvesting gradients
C05 – Isolation of human peripheral blood mononuclear cells by flotation (iodixanol mixer technique)   M05 – Analysis of gradients   S05 – Homogenization of mammalian tissues   V05 – Analysis of gradients
C06 – Isolation of human peripheral blood mononuclear cells by flotation (low density iodixanol density barrier)   M06 – Isolation of plasmid DNA in self-generated gradients of iodixanol using DAPI as a fluorescent marker   S06 – Homogenization of mammalian cells   V06 – Purification of viruses and viral vectors using OptiPrep™
C07 – Isolation of rat blood mononuclear cells by flotation (iodixanol mixer technique)   M07 – Analysis of mammalian and non-mammalian HDL, LDL and VLDL   S07 – Differential centrifugation of homogenates   V07 – Purification of Group I (ds)DNA viruses: adenovirus and removal of helper virus
C08 – Isolation of mononuclear cells from mouse blood by flotation (iodixanol mixer technique)   M08 – Fractionation of plasma lipoprotein sub-classes   S08 – Harvesting gradients   V08 – Purification of Group I (ds)DNA viruses:
C09 – Isolation of ruminant and equine peripheral blood mononuclear cells in iodixanol gradients   M09 – Analysis of protein complex formation, microtubules and cytoskeleton   S09 – Analysis of gradients   Herpesviridae and Baculoviridae in a self-generated gradient
C10 – Separation of monocytes from a leukocyte-rich plasma by flotation through a discontinuous iodixanol gradient   M10 – Separation of proteoliposomes from proteins and liposome-encapsulated macromolecules from liposomes   S10a – Purification of nuclei from tissues and cells in iodixanol gradients – a methodological review   V09 – Purification of Group I (ds)DNA viruses:
C11 – Isolation of a monocyte-rich fraction from whole human blood by iodixanol barrier flotation   M11 – Analysis of protein size in pre-formed gradients   S10 – Isolation of nuclei from animal and plant sources and cultured cells   Herpesviridae and Asfaviridae in a pre-formed gradient
C12 – Isolation of human polymorphonuclear leukocytes (granulocytes) from a leukocyte rich plasma in a discontinuous iodixanol gradient   M12 – Analysis of proteins in small volume self-generated gradients   S11 – Purification of mammalian peroxisomes in a continuous gradient   V10 – Purification of Group I (ds)DNA viruses: human papillomavirus
C13 – Isolation (removal) of human platelets (thrombocytes) on an iodixanol barrier   M13 – Fractionation of nucleic acids and nucleic acid-protein complexes   S12 – Purification of mammalian peroxisomes on a discontinuous gradient or density barrier   V11 – Purification of Group I (ds)DNA viruses: Polyomaviridae
C14 – Removal of non-viable cells from a cell suspension   Macromolecules-indexes-Serumwerk   S13 – Purification of mammalian peroxisomes in a self-generated gradient   V12 – Purification of Group I (ds)DNA viruses: Poxviridae
C15 – Fractionation of a mixed population of cells  
Collected literature references for specific macromolecules
   S14a – Purification and analysis of mammalian mitochondrial fractions   V13 – Purification of Group I (ds)DNA viruses (non-mammalian sources): Iridoviridae
C16 – Purification of Islets of Langerhans from porcine, primate and rodent pancreas in a discontinuous iodixanol gradient     S14 – Purification of mammalian mitochondria in a discontinuous gradient   V14 – Purification of Group II (ss)DNA viruses: Parvoviridae: recombinant adenoassociated virus (rAAV) and avian AAV
C17 – Purification and processing of animal (non-human) and avian spermatozoa   RM02 – Proteo- and DNA-liposomes – methodology and bibliography   S15 – Light mitochondrial fraction analysis (mitochondria, lysosomes and peroxisomes) in continuous gradients   V15 – Purification of Group II (ss)DNA viruses: Protoparvovirus – murine minute virus
C18 – Purification of viable human spermatozoa in iodixanol or Nycodenz® gradients       S16 – Light mitochondrial fraction analysis (mitochondria, lysosomes and peroxisomes) in self-generated gradients   V16 – Purification of Group II (ss)DNA viruses: Parvovirinae – parvovirus
C19 – Purification of intact plant protoplasts by density gradient flotation       S17 – Purification of yeast spheroplast mitochondria   V17 – Purification of Group III (ds)RNA viruses: Reoviridae
C20 – Purification of leukocyte fractions from rodent/rabbit peritoneal exudates       S18 – Fractionation of rough and smooth endoplasmic reticulum in self-generated gradients   V18 – Purification of Class IV ((+)ssRNA) viruses: Caliciviridae family
C21 – Dendritic cells from tissues by flotation through a low-density barrier       S19 – Fractionation of rough and smooth endoplasmic reticulum and subfractionation of ER from cultured mammalian cells   V19 – Purification of Group IV ((+)ss) RNA viruses: Flaviviridae family and hepatitis E in pre-formed gradients
C22 – Isolation of dendritic cells from tissues by a mixer technique       S20 – Fractionation of smooth and rough endoplasmic reticulum and separation from Golgi (and other organelles)   V20 – Purification of Group IV ((+)ss) RNA viruses: Flaviviridae family: hepatitis C particles in a self-generated gradient
C23 – Isolation of a mouse motoneuron enriched fraction from spinal cord on a density barrier       S21 – Analysis of ER, plasma membrane, endosomes, Golgi, ERGIC, TGN from mammalian cells and tissues in continuous iodixanol gradients (1.5-3 h spin)   V21 – Purification of Group IV ((+)ss) RNA viruses: Nidovirales: Coronaviridae and Arteriviridae
C24 – Isolation of a progenitor cell-enriched fraction       S22 – Analysis of ER, plasma membrane, endosomes, Golgi, ERGIC and TGN from mammalian cells and tissues in continuous gradients (16-18 h spin)   V22 – Purification of Group IV ((+)ss) RNA viruses: Togaviridae: Alphavirus and Rubivirus
C25 – Hepatic non-parenchymal (stellate, Kupffer and endothelial) cells – a short methodological survey       S23 – Analysis of ER, plasma membrane, endosomes, Golgi, ERGIC and TGN from mammalian cells and tissues by flotation in discontinuous gradients   V23 – Purification of Group V ((-)ss) RNA viruses: Arenaviruses: Lassa virus, Tacaribe virus, Junin virus, Lymphocytic choriomeningitic virus (LCV)
C26 – Purification of hepatic non-parenchymal cells on a density barrier       S24 – Analysis of ER, plasma membrane, endosomes, Golgi, ERGIC and TGN from mammalian cells and tissues by sedimentation in discontinuous gradients   V24 – Purification of Group V ((-)ss) RNA viruses: Bunyaviridae – Hantavirus, Orthobunyavirus and Phlebovirus
C27 – Preparation of stellate cells from liver and pancreas       S25 – Analysis of ER, plasma membrane, endosomes, Golgi, ERGIC and TGN from cells and tissues in sedimentation velocity gradients   V25 – Purification of Group V ((-)ss) RNA viruses: Ebola virus capsid assembly analysis and purification of Ebola pseudovirus in self-generated gradients
C28 – Enrichment of hepatic Kupffer cells in a discontinuous gradient       S26 – Isolation of plasma membrane from cardiac muscle   V26 – Purification of Group V ((-)ss) RNA viruses: Orthomyxoviridae
C29 – Isolation of cells from pulmonary tissue Endothelial from epithelial cells, type I epithelial cells and macrophages       S27 – Fractionation of plasma membrane and plasma membrane domains using cationic colloidal silica   V27 – Purification of Group V ((-)ss) RNA viruses: Rhabdoviridae: Rabies virus (Lyssavirus) and vesicular stomatitis virus
C30 – Isolation of rat alveolar type II pneumocytes, lymphoid and myeloid cells       S28 – Fractionation of brush border and basolateral plasma membrane domains from intestinal (ileal) mucosa   V28 – Purification of Group V ((-)ss) RNA viruses: Paramyxoviridae – Paramyxovirinae and Pneumovirinae
C31 – Purification of protozoa       S29 – Fractionation of apical and basolateral plasma membrane domains from Caco-2 cells   V29 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Alpharetrovirus: Rous sarcoma virus
C32 – Purification of malarial parasites (Plasmodium falciparum, Plasmodium berghei, Plasmodium vivax, and Plasmodium yoelii)       S30 – Fractionation of plasma membrane microdomains of the brush border from renal cortex tissue and from glomeruli (slit diaphragms)   V30 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Betaretrovirus: Mason-Pfizer Monkey virus
C33 – Purification of Toxoplasma gondii from cell cultures (Part A) Separation of sporocysts and oocyst walls (Part B)       S31 – Analysis of plasma membrane domains and apical junctional complex from polarized epithelial cells in a self-generated gradient   V31 – Purification of Group VI (ss)RNA-RT viruses: Retroviridae: Deltaretrovirus, human T-cell lymphotropic virus (HTLV-1) and human endogenous retrovirus
C34 – Fractionation and analysis of normal erythrocytes, sickle cells and reticulocytes; removal of erythrocytes from blood and bone marrow       S32 – Purification of lipid rafts from cells and tissues (detergent method)   V32 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Gammaretrovirus: murine oncornavirus
C35 – Isolation of neuroglia, inflammatory and glial cells from neural tissue       S33 – Purification of lipid rafts from cells and tissues (detergent-free method)   V33 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Gammaretrovirus: Moloney murine leukemia virus
C36 – Isolation of brain motoneurons       S34 – Purification of caveolae from cells and tissues by sonication of a plasma membrane fraction (detergent-free)   V34 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Lentivirus: Human immunodeficiency virus – 1 (HIV-1) and lentivirus vectors
C37 – Isolation of renal cells: (1) Proximal tubule cells and (2) Interstitial cells and thin loop of Henlé cells       S35 – Separation of membrane-bound and cytosolic proteins (flotation through a discontinuous gradient)   V35 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Spumaretrovirinae: Foamy viruses
C38 – (I) Maintenance of cells in suspension for chemical/physical measurements (II) Microfluidic cell encapsulation/cell sorting       S36 – Separation of membrane vesicles and cytosol in a self-generated gradient   V36 – Purification and analysis of Group VII (ds RNA-RT): Hepadnaviridae: Orthohepadnavirus: hepatitis B virus
C39 – Purification of bacteria from soil, stream-water, clinical specimens and food       S37 – Determination of the cytosolic or membrane location of a large protein complex   V37 – Purification of viruses from non-mammalian cells and tissues
C40 – Isolation of mononuclear cells from tissues       S38 – Isolation of secretory granules from pancreatic cells   V38 – Purification and analysis of bacteriophages
C41 – Isolation of dendritic cells from tissues by sedimentation on to a density barrier       S39 – Isolation of a granule fraction from eosinophils   Virus-index-Serumwerk
C42 – Purification of macrophages and foam cells       S40a – Preparation of synaptosomes, synaptoneurosomes, neuromelanin granules and synaptic vesicles – a methodological survey   Collected literature references for specific viruses
C43 – Isolation of mononuclear cells from rat, mouse and rabbit blood on a density barrier       S40 – Isolation of vesicular and granular fractions from various sources  
C44 – Isolation of polymorphonuclear leukocytes (granulocytes) from rat, mouse, guinea pig and rabbit blood       S41 – Resolution of smooth endoplasmic reticulum (SER), SER domains, study of SER communication with other organelles and lipid droplets   RV02-1 – GROUP II VIRUSES
C45 – Isolation of peripheral blood mononuclear cells from non-human primates       S42 – Endocytosis analysis – a review of density gradient methods   RV02-2 – GROUP II VIRUSES – rAAV
C46 – Isolation of monocytes from a human leukocyte-rich plasma on a density barrier       S43 – Lysosomes – a methodological review   RV03 – GROUP III VIRUSES
C47 – Purification of enterochromaffin-like (ECL) cells from gastric mucosa       S44 – Endocytosis of ligands: analysis in sedimentation velocity gradients   RV04 – GROUP IV VIRUSES
C48 – Fractionation of parietal and chief cells from the gastric mucosa       S45 – Endocytosis of ligands: fractionation of clathrin-coated vesicles, endosomes and lysosomes by buoyant density in a self-generated gradient   RV05 – GROUP V VIRUSES
C49 – Purification of bacteria and bacterial minicells from cultured cells       S46 – Endocytosis in cultured cells: analysis of endosomes, lysosomes and plasma membrane by buoyant density   RV06 – GROUP VI VIRUSES
C50 – Fractionation of coelomocyte cell populations from sea urchins       S47a – Intracellular exocytic vesicle trafficking and exocyst complex – a short methodological summary   RV07 – GROUP VII VIRUSES
C51 – Analysis of gradients       S47 – Analysis of exocytosis, exocyst function and plasma membrane domain targeting in (A) self-generated and (B) pre-formed gradients   RV08 – UNCLASSIFIED VIRUSES & SURVEYS
Cell-index-Serumwerk       S48 – Analysis of membrane trafficking and intracellular signaling in selfgenerated gradients    
Collected literature references for specific cell types
       S49 – Analysis of membranes from Drosophila    
      S50 – Fractionation of acidocalcisomes from trypanosomes (and other protozoa) and Gram-negative bacteria    
RC02 – Purification of platelets from whole blood/removal from blood leukocyte preparations       S51 – Isolation of optical system membranes and structures    
RC03 – Viable/non-viable cell separation       S52 – Fractionation of vacuoles, pre-vacuoles, vacuolar, subvacuolar vesicles, secretory vesicles and Cvt vesicles from yeast spheroplasts    
RC04 – Purification of pancreatic islets       S53 – Fractionation of ER, Golgi, TGN, endosomes and vacuoles from yeast spheroplasts    
RC05 – Dendritic cells from blood and tissues       S54 – Endocytosis in rat liver: analysis of lysosome and late endosome events using a two-phase Nycodenz®-polysucrose gradient    
RC06 – Isolation of cells from brain and spinal cord       S55 – Isolation of mammalian lysosomes in discontinuous gradients    
RC07 – Hepatic and pancreatic stellate cells       S56 – Analysis of mammalian lysosomes (ER, endosomes and plasma membrane) in continuous gradients    
RC08 – Hepatic non-parenchymal cells: Kupffer cells, sinusoidal endothelial cells (and other liver cell types)       S57 – Isolation of peroxisomes from yeast spheroplasts    
RC09 – Pulmonary cells       S58 – Isolation of organelles from protozoa and amoeba    
        S59 – Isolation of organelles from algae and fungi    
        S60 – Isolation of organelles from plants and plant cells    
        S61 – Membrane trafficking and proteomic analysis of plants and plant cells    
        S62 – Extracellular vesicles from non-mammalian sources    
        S63 – Mammalian cell exosomes and other microvesicles from conditioned medium    
        S64 – Isolation of plasma membrane from cultured cells by flotation through a discontinuous gradient    
        Subcellular-indexes-Serumwerk    
       
Collected literature references for specific organelles and membranes
    
           
        RS02 – Purification and analysis of peroxisomes    
        RS03 – Purification of mitochondria from mammalian sources    
        RS04 – Purification of and analysis of lysosomes    
        RS05 – Analysis of membrane trafficking in mammalian tissues and cells: fractionation of ER, Golgi, TGN, PM and endosomes    
        RS06 – Lipid rich detergent-resistant domains (mammalian sources)    
        RS07 – Detergent-free strategy for lipid raft isolation from mammalian cells, tissues and organelles    
        RS08 – Purification of caveolae in gradients prepared from OptiPrep™    
        RS09 – Lipid-rich membranes from non-mammalian sources    
        RS10 – Mammalian (including all chordata) cell exosomes and other microvesicles from, tissues, cells and conditioned medium    
        RS11 – Extracellular vesicles from non-mammalian sources    
        RS12 – Endocytosis – studies on various tissue and cell types    
        RS13 – Resolution of soluble cytosolic proteins from membrane vesicles and organelles    
        RS14 – Purification of organelles and membranes from non-mammalian eukaryotes    
        RS15 – Purification of subcelluar organelles and membrane compartments from Saccharomyces cerevisiae – a bibliography