Anti UmuD (DNA Polymerase V) pAb (Rabbit, Antiserum)

Cosmo Bio LTD

Catalog No: BAM-61-011-EX

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Application: WB 
Clonality: Polyclonal 
Host: Rabbit 
Purification: Serum 
Reactivity: E. coli 

The products of umuD, umuC, and recA genes (SOS genes) are required for mutagenesis induced by radiation or chemical agents. Transcription of these SOS genes is repressed by a repressor, LexA protein in uninduced cells (Ref.2). Exposure of cells to DNA-damaging agents activates RecA protein to promote proteolytic cleavage of LexA protein. Inactivation of LexA protein by the cleavage consequently derepresses the SOS genes, umuD, C and recA. UmuD protein is then auto-cleaved, which is promoted by RecA protein ssDNA in a ATP-dependent manner (Ref.1). The processed UmuD protein is the active form for mutagenesis and the UmuD-UmuC complex functions as an error-prone translesion DNA polymerase (Ref.3). The molecular weight of the intact UmuD is 17kD and the proteolytically processed active form is 14kD