Anti TFG2p TFIIF (Transcription Factor II) Middle Subunit pAb (Rabbit, Antiserum)

Cosmo Bio LTD

Catalog No: BAM-62-019-EX

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Application: WB 
Clonality: Polyclonal 
Host: Rabbit 
Purification: Serum 
Reactivity: S. cerevisiae 
BACKGROUND The basal transcription factor TFIIF binds with RNA polymerase II and takes part in the transcription initiation reaction and the elongation reaction. The budding yeast TFIIF is composed of three subunits, Tfg1p (735aa), Tfg2p (400aa) and Taf14p (244aa). The product is prepared by immunizing rabbit with recombinant protein which was over-expressed in E. coli with a plasmid carrying the entire region of Tfg2p protein (1-400aa) of budding yeast, and purified by chromatography. Using this antiserum in Western blotting, the band of 54 kD corresponding to Tfg2p was obtained from the extract of yeast cells (Fig. 1).