![Immunostaining with anti NAPlr-FITC antibody. Primary Antibody: anti NAPlr-FITC, 1:5 dilution [0.26 mg/mL]). Tissue: Section of unfixed frozen renal biopsy tissue from an APSGN patient (positive control). 1. Air dry unfixed renal biopsy frozen section. 2. Wash with PBS (3 min each x 2). 3. 1-hour ambient temperature incubation with primary antibody (anti NAPlr-FITC, 1:5 dilution [0.26 mg/mL]). 4. Wash with PBS (5 min each x 3). 5. Mount with mounting medium for fluorescent staining. 6. Observe stained sections by fluorescent microscope.](https://cdn11.bigcommerce.com/s-ydswqc5qsc/images/stencil/500x659/products/29841/120396/cac-tmu-pa001x_anti-nephritis-associated-plasmin-receptor-naplr-pab_7837__62172.1620917192.png?c=2 "Immunostaining with anti NAPlr-FITC antibody. Primary Antibody: anti NAPlr-FITC, 1:5 dilution [0.26 mg/mL]). Tissue: Section of unfixed frozen renal biopsy tissue from an APSGN patient (positive control). 1. Air dry unfixed renal biopsy frozen section. 2. Wash with PBS (3 min each x 2). 3. 1-hour ambient temperature incubation with primary antibody (anti NAPlr-FITC, 1:5 dilution [0.26 mg/mL]). 4. Wash with PBS (5 min each x 3). 5. Mount with mounting medium for fluorescent staining. 6. Observe stained sections by fluorescent microscope.")
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Application: IHC(f), IFClonality: Polyclonal
Conjugation: Fluorescein
Host: Rabbit
Purification: Ig-PG
Reactivity: Streptococcus
Acute post-streptococcal glomerulonephritis (APSGN) is an infection- triggered glomerulonephritic disease (Reference 1). Nephritis-Associated Plasmin receptor (NAPlr) is an intracellular component of hemolytic streptococcus that induces nephritis. Glomerular NAPlr deposition is frequently found in infection-related glomerulonephritis (IRGN), especially in glomerulonephritis that is triggered by streptococcal infection (i.e. streptococcal-infection related nephritis [SIRN]). Glomerular NAPlr deposition is not found in non-infection-related nephritis.
Therefore, Immunostaining using anti-NAPlr antibody is ideal for identifying IRGN and SIRN (References 2-5). Although a distinct commercially available anti-NAPlr monoclonal antibody (1F10) has been in use, it is claimed to suffer from low sensitivity in Immunostaining. By contrast, staining sensitivity is better with polyclonal anti-NAPlr antibodies. However, stable generation and supply of this pAb is difficult because of the complicated step of NAPlr extraction from streptococcal particles. This new polyclonal antibody against an NAPlr peptide was established to balance both satisfactory sensitivity in Immunostaining with stable antibody production.
References:
1) Oda T, Up-to-date findings on infection-related glomerulonephritis. æ±å»å¤§èª73(4):355-363, 2015.
2) Yoshizawa N, Yamakami K, Fujino M, et al. (2004) Nephritis-associated plasmin receptor and acute poststreptococcal glomerulonephritis. J Am Soc Nephrol. 15:1785-93.
3) Oda T, Yamakami K, Yoshizawa N, et al. (2005) Glomerular plasmin-like activity in relation to nephritis-associated plasmin receptor in acute poststreptococcal glomerulonephritis. J Am Soc Nephrol. 16:247-54.
4) Oda T, Yoshizawa N, Yamakami K, et al. (2010) Localization of nephritis-associated plasmin receptor in acute poststreptococcal glomerulonephritis. Hum Pathol. 41(9):1276-85.
5) Oda T, Yoshizawa N, Yamakami K, et al. (2012) The role of nephritis-associated plasmin receptor (NAPlr) in glomerulonephritis associated with streptococcal infection. J Biomed Biotechnol. 2012:417675.
| Documents & Links for Anti Nephritis-Associated Plasmin receptor (NAPlr/Streptococcal GAPDH) pAb (Rabbit, Purified Ig) | |
| Datasheet | Anti Nephritis-Associated Plasmin receptor (NAPlr/Streptococcal GAPDH) pAb (Rabbit, Purified Ig) Datasheet |
| Documents & Links for Anti Nephritis-Associated Plasmin receptor (NAPlr/Streptococcal GAPDH) pAb (Rabbit, Purified Ig) | |
| Datasheet | Anti Nephritis-Associated Plasmin receptor (NAPlr/Streptococcal GAPDH) pAb (Rabbit, Purified Ig) Datasheet |