Anti DNA Replication Licensing Factor MCM7 (CDC47) pAb (Rabbit, Purified IgG)

Cosmo Bio LTD

Catalog No: BAM-70-120EX

Please click here to view other Bio Academia (BAM) Products

Application: IP, IF, WB, ChIP, FC, ICC 
Clonality: Polyclonal 
Host: Rabbit 
Purification: IgG 
Reactivity: Hamster, Mouse, Rat, Human 

Function: MCM7 (human; 718 aa, 80 kDa) acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

Applications

1) Western blotting (1/1,000~1/5,000 dilution).
2) Immunoprecipitation (assay dependent)
3) Immunofluorescence staining (1/200~1/1,000 dilution). Other applications have not been tested. Immunogen: Purified His6-tagged human MCM7 protein encompassing 562 -719 amino acids. Reactivity: Reacts with human mouse, rat and hamster. Not tested in other species. Product: Purified IgG from the rabbit antiserum. 1 mg/ml in PBS, 50% glycerol, filter-sterilized. Azide- and carrier-free. Size: 100 ug Storage: Shipped at 4℃. Upon arrival, spin-down, aliquot and store at -20℃. Data Link UniProtKB/Swiss-Prot P33993 MCM7_HUMAN

References:

1. Fujita M et al. (1996) hCDC47, a human member of the MCM family. Dissociation of the nucleus-bound form during S phase. J Biol Chem. 271:4349-54. PMID 8626784 Free Article. WB, IP, IF
2. Fujita M. et al. (1997) In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP. J Biol Chem. 272:10928-35. PMID 9099751 Free Article. WB, IP
3. Fujita M. et al. (2002) Nuclear organization of DNA replication initiation proteins in mammalian cells. J Biol Chem. 277:10354-61. PMID 11779870 Free Article. WB, IP, IF