Anti CD63 Antigen (LAMP-3/Tspan-30) mAb (Clone 8A12)

Cosmo Bio

Catalog No.: CAC-SHI-EXO-M02-50

Product Description

CD63 (also known as LAMP-3, Melanoma-associated antigen ME491, TSPAN30, MLA1 and OMA81H) is a cell surface glycoprotein which belongs to the tetraspanin superfamily. CD63 is known to complex with integrins. CD63 is expressed on activated platelets, monocytes and macrophages and is weakly expressed on granulocytes, T cells and B cells. Importantly, it is found on the surface of exosomes.

Exosomes are cell-derived vesicles bounded by a lipid bilayer membrane and exhibiting a diameter of 50 to 150 nm. They are secreted from cultured cells and are observed in body fluids such as saliva, blood, urine, amniotic fluid, malignant ascites. Recent studies indicate that exosomes contain various proteins and RNAs, suggesting a role in information transfer between cells.

This monoclonal antibody can be used to immunoprecipitate exosomes from serum and culture supernatants.

References

1) Yoshioka Y et al., Nat Commun. 2014 Apr 7;5:3591. doi: 10.1038/ncomms4591.
2) N Nishida-Aoki et al., Mol Ther. 2017 Jan 4;25(1):181-191. doi: 10.1016/j.ymthe.2016.10.009.
3) Saito S et al., Sci Rep. 2018 Mar 5;8(1):3997. doi: 10.1038/s41598-018-22450-2.

Product Specifications

• Application: IP, WB, IF, FC
• Reactivity: Human
• Clonality: Monoclonal IgG2a (Clone No.: 8A12)
• Host: Mouse
• Immunogen: Human CD63 (a.a 104-202)

Citations for Anti CD63 Antigen (LAMP-3/Tspan-30) mAb (Clone 8A12) – 5 Found

Jingushi, Kentaro; Uemura, Motohide; Nakano, Kosuke; Hayashi, Yujiro; Wang, Cong; Ishizuya, Yu; Yamamoto, Yoshiyuki; Hayashi, Takuji; Kinouchi, Toshiro; Matsuzaki, Kyosuke; Kato, Taigo; Kawashima, Atsunari; Ujike, Takeshi; Nagahara, Akira; Fujita, Kazutoshi; Ueda, Koji; Tsujikawa, Kazutake; Nonomura, Norio. Leukocyte‑associated immunoglobulin‑like receptor 1 promotes tumorigenesis in RCC. Oncology Reports. 2019;41(2):1293-1303. 

Matsumura, Sachiko; Minamisawa, Tamiko; Suga, Kanako; Kishita, Hiromi; Akagi, Takanori; Ichiki, Takanori; Ichikawa, Yuki; Shiba, Kiyotaka. Subtypes of tumour cell-derived small extracellular vesicles having differently externalized phosphatidylserine. Journal Of Extracellular Vesicles. 8(1):1579541. 

Yeatman, Jason D; Richie-Halford, Adam; Smith, Josh K; Keshavan, Anisha; Rokem, Ariel. A browser-based tool for visualization and analysis of diffusion MRI data. Nature Communications. 2018;9(1):940. 

Genovese, Elizabeth A; Fish, Larry; Chaer, Rabih A; Makaroun, Michel S; Baril, Donald T. Risk stratification for the development of respiratory adverse events following vascular surgery using the Society of Vascular Surgery's Vascular Quality Initiative. Journal Of Vascular Surgery. 2017;65(2):459-470. 

Taylor, Gary S; Kent, Deborah S. Potential economic pests of solanaceous crops: a new species of Solanum-feeding psyllid from Australia and first record from New Zealand of Acizzia solanicola (Hemiptera: Psyllidae). Zootaxa. 2013;3613:257-73.