2-Deoxyglucose (2DG) Uptake Measurement Kit

Cosmo Bio

Catalog No.: CSR-OKP-PMG-K01H

Background

2-deoxyglucose uptake measurement is a reliable approach for estimating glucose uptake in cells and tissues. Based on a published method, the 2-Deoxyglucose (2DG) Uptake Measurement Kit facilitates highly sensitive and direct enzymatic 2DG quantitation, obviating the need for radioisotopes and their attendant issues.

Measurement of 2-deoxyglucose (2DG) uptake in tissues and cells is a reliable approach for estimating glucose uptake and thereby to explore the regulation of glucose metabolism and mechanisms of insulin resistance. While assays employing radioisotope-labeled 2DG are often used to measure 2DG uptake in vivo and in vitro, the use of radioisotopes is not possible without problems including permitting, handling, and disposal. Furthermore, in actual practice, radioactive assay protocols for 2DG uptake require a corrective separation step to account for labeled 2DG that remains in extracellular fluids and can lead to substantial variation. Such problems are obviated by the enzymatic method of 2DG detection employed by this kit, based on published methods (Saito K and Minokoshi Y, et al. Analytical Biochem 412: 9-17, 2011).

Features

• No need for radioisotopes (RIs) or radiation counting instrumentation
• Photometric readout on standard microplate readers
• Direct measurement of 2DG6P accumulation in cells
• High sensitivity achieved by a recycling enzymatic amplification reaction

Assay principle in five key steps

1. Oxidation of glucose-6-phosphate (G6P) with a low concentration of G6P dehydrogenase (G6PDH) with NAD+ to eliminate endogenous G6P in target cells
2. Elimination of NAD(P)H with HCl, which removes endogenous NAD(P)H as well as NADH produced in step 1
3. Generation of NADPH through oxidation of 2DG6P in cells with a high concentration of G6PDH, the generated NADPH being used for quantification of 2DG6P
4. Elimination of NAD(P)+ and G6PDH that remains after Step 2 with NaOH
5. Recycling amplification reaction for the small amount of NADPH generated, and quantification of 2DG6P using a photometric microplate reader at 420 nanometers wavelength

Citations for 2-Deoxyglucose (2DG) Uptake Measurement Kit – 96 Found

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Yao, Yilin; Tian, Shen; Li, Ningxin; Yang, Yanzhou; Zhang, Cheng. Effects of omega-3 polyunsaturated fatty acids on cellular development in human ovarian granulosa tumor cells (KGN). Frontiers In Nutrition. 9:1017072. 

Lee, Da Som; An, Tae Hyeon; Kim, Hyunmi; Jung, Eunsun; Kim, Gyeonghun; Oh, Seung Yeon; Kim, Jun Seok; Chun, Hye Jin; Jung, Jaeeun; Lee, Eun-Woo; Han, Baek-Soo; Han, Dai Hoon; Lee, Yong-Ho; Han, Tae-Su; Hur, Keun; Lee, Chul-Ho; Kim, Dae-Soo; Kim, Won Kon; Park, Jun Won; Koo, Seung-Hoi; Seong, Je Kyung; Lee, Sang Chul; Kim, Hail; Bae, Kwang-Hee; Oh, Kyoung-Jin. Tcf7l2 in hepatocytes regulates de novo lipogenesis in diet-induced non-alcoholic fatty liver disease in mice. Diabetologia. 2023;66(5):931-954. 

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Zhong, Guoqing; Yang, Qingping; Wang, Yihua; Liang, Yuan; Wang, Xiaojing; Zhao, Dongli. Long noncoding RNA X-inactive specific transcript (lncRNA XIST) inhibits hepatic insulin resistance by competitively binding microRNA-182-5p. Immunity, Inflammation And Disease. 2023;11(11):e969. 

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Xu, Xin; Wang, Ruotong; Pei, Linlin; Wang, Quanfeng; Liu, Chunjie. Glucose Transport by Follicle-Stimulating Hormone Is Mediated Through the Akt/FOXO1 Pathway in Ovine Granulosa Cells. Veterinary Medicine And Science. 2025;11(2):e70294. 

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Huang, Dazhuang; Haba, Daijiro; Tomida, Sanai; Takizawa, Chihiro; Qin, Qi; Kataoka, Yukie; Mugita, Yuko; Sanada, Hiromi; Nakagami, Gojiro. Piezo1 mediates calcium ion influx, glucose transporter 4 translocation, and glucose uptake in adipocytes under low-frequency vibration. Drug Discoveries & Therapeutics. 2025;19(4):237-244. 

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Kojima, Fusako; Morishita, So; Uda, Shinsuke; Yutsudo, Noriko; Ogawa, Yoshihiro; Kubota, Hiroyuki. Evaluation of the insulin-dependent and -independent hypoglycemic effects and understanding their breakdown in the progression of obesity using mice. Plos One. 20(12):e0337739.