2-Deoxyglucose (2DG) Uptake Measurement Kit

Cosmo Bio LTD

Catalog No: CSR-OKP-PMG-K01H

Click here to view more information about the 2-Deoxyglucose (2DG) Uptake Measurement Kit

2-deoxyglucose uptake measurement is a reliable approach for estimating glucose uptake in cells and tissues. Based on a published method, the 2-Deoxyglucose (2DG) Uptake Measurement Kit facilitates highly sensitive and direct enzymatic 2DG quantitation, obviating the need for radioisotopes and their attendant issues.

Measurement of 2-deoxyglucose (2DG) uptake in tissues and cells is a reliable approach for estimating glucose uptake and thereby to explore the regulation of glucose metabolism and mechanisms of insulin resistance. While assays employing radioisotope-labeled 2DG are often used to measure 2DG uptake in vivo and in vitro, the use of radioisotopes is not possible without problems including permitting, handling, and disposal. Furthermore, in actual practice, radioactive assay protocols for 2DG uptake require a corrective separation step to account for labeled 2DG that remains in extracellular fluids and can lead to substantial variation. Such problems are obviated by the enzymatic method of 2DG detection employed by this kit, based on published methods (Saito K and Minokoshi Y, et al. Analytical Biochem 412: 9-17, 2011).

Features

• No need for radioisotopes (RIs) or radiation counting instrumentation
• Photometric readout on standard microplate readers
• Direct measurement of 2DG6P accumulation in cells
• High sensitivity achieved by a recycling enzymatic amplification reaction

Assay principle in five key steps

1. Oxidation of glucose-6-phosphate (G6P) with a low concentration of G6P dehydrogenase (G6PDH) with NAD+ to eliminate endogenous G6P in target cells
2. Elimination of NAD(P)H with HCl, which removes endogenous NAD(P)H as well as NADH produced in step 1
3. Generation of NADPH through oxidation of 2DG6P in cells with a high concentration of G6PDH, the generated NADPH being used for quantification of 2DG6P
4. Elimination of NAD(P)+ and G6PDH that remains after Step 2 with NaOH
5. Recycling amplification reaction for the small amount of NADPH generated, and quantification of 2DG6P using a photometric microplate reader at 420 nanometers wavelength

Citations for 2-Deoxyglucose (2DG) Uptake Measurement Kit – 30 Found

Gherardi et al. 2019. Loss of mitochondrial calcium uniporter rewires skeletal muscle metabolism and substrate preference. Cell Death Differ. 26(2):362-381.   , Journal

Kunii et al. 2016. Opposing roles for SNAP23 in secretion in exocrine and endocrine pancreatic cells. J Cell Biol. 215(1):121-138.   , Journal

Uruno et al. 2016. Nrf2-Mediated Regulation of Skeletal Muscle Glycogen Metabolism. Mol Cell Biol. 36(11):1655-72.   , Journal

Chee et al. 2016. Relative Contribution of Intramyocellular Lipid to Whole-Body Fat Oxidation Is Reduced With Age but Subsarcolemmal Lipid Accumulation and Insulin Resistance Are Only Associated With Overweight Individuals. Diabetes. 65(4):840-50.   , Journal

Albert et al. 2016. mTORC2 sustains thermogenesis via Akt-induced glucose uptake and glycolysis in brown adipose tissue. EMBO Mol Med. 8(3):232-46.   , Journal

Guridi et al. 2015. Activation of mTORC1 in skeletal muscle regulates whole-body metabolism through FGF21. Sci Signal. 8(402):ra113.   , Journal

Ijuin et al. 2015. Phosphatidylinositol 3,4,5-Trisphosphate Phosphatase SKIP Links Endoplasmic Reticulum Stress in Skeletal Muscle to Insulin Resistance. Mol Cell Biol. 36(1):108-18.   , Journal

Tamura et al. 2015. Role of plasminogen activator inhibitor-1 in glucocorticoid-induced diabetes and osteopenia in mice. Diabetes. 64(6):2194-206.   , Journal

Tamura et al. 2015. Role of plasminogen activator inhibitor-1 in glucocorticoid-induced diabetes and osteopenia in mice. Diabetes. 64(6):2194-206.   , Journal

Jayakody et al. 2015. SUMO expression shortens the lag phase of Saccharomyces cerevisiae yeast growth caused by complex interactive effects of major mixed fermentation inhibitors found in hot-compressed water-treated lignocellulosic hydrolysate. Appl Microbiol Biotechnol. 99(1):501-15.   , Journal

Ijuin et al. 2015. Improvement of insulin signaling in myoblast cells by an addition of SKIP-binding peptide within Pak1 kinase domain. Biochem Biophys Res Commun. 456(1):41-6.   , Journal

López-Vicario et al. 2015. Inhibition of soluble epoxide hydrolase modulates inflammation and autophagy in obese adipose tissue and liver: role for omega-3 epoxides. PNAS. 112(2):536-41.   , Journal

Hiyoshi et al. 2014. Neurophysiologic and antipsychotic profiles of TASP0433864, a novel positive allosteric modulator of metabotropic glutamate 2 receptor. J Pharmacol Exp Ther. 351(3):642-53.   , Journal

Hiyoshi et al. 2014. Neurophysiologic and antipsychotic profiles of TASP0433864, a novel positive allosteric modulator of metabotropic glutamate 2 receptor. J Pharmacol Exp Ther. 351(3):642-53.   , Journal

Baker et al. 2014. Resveratrol protects against polychlorinated biphenyl-mediated impairment of glucose homeostasis in adipocytes. J Nutr Biochem. 24(12):2168-74.   , Journal

Park et al. 2014. SIK2 is critical in the regulation of lipid homeostasis and adipogenesis in vivo. Diabetes. 63(11):3659-73.   , Journal

Tamura et al. 2014. Genome-wide analysis of histone modifications in human endometrial stromal cells. Mol Endocrinol. 28(10):1656-69.   , Journal

Watanabe et al. 2014. Aculeatin, a coumarin derived from Toddalia asiatica (L.) Lam., enhances differentiation and lipolysis of 3T3-L1 adipocytes. Biochem Biophys Res Commun. 453(4):787-92.   , Journal

Yoshioka et al. 2014. Water-Soluble C60-(OH)24 Fullerene Hydroxide Protects against the Catabolic Stress-Induce Downregulation of Chondrocyte Activity in Osteoarthritis. J Nanotechnology and Smart Materials. 1(401):.  Journal

Takahashi et al. 2014. Myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double-muscled (DM) and normal-muscled (NM) Japanese shorthorn cattle. Domest Anim Endocrinol. 48:62-8.   , Journal

Ohminami et al. 2014. Dietary combination of sucrose and linoleic acid causes skeletal muscle metabolic abnormalities in Zucker fatty rats through specific modification of fatty acid composition. J Clin Biochem Nutr. 55(1):15-25.   , Journal

Salvador-Adriano et al. 2014. Insulin sensitivity is inversely related to cellular energy status, as revealed by biotin deprivation. Am J Physiol Endocrinol Metab. 306(12):E1442-8.   , Journal

Yamada et al. 2014. Hydroxyeicosapentaenoic acids from the Pacific krill show high ligand activities for PPARs. J Lipid Res. 55(5):895-904.   , Journal

Shimizu et al. 2014. Vascular rarefaction mediates whitening of brown fat in obesity. J Clin Invest. 124(5):2099-112.   , Journal

Morita et al. 2014. Paternal allele influences high fat diet-induced obesity. PLoS One. 9(1):e85477.   , Journal

Woo et al. 2013. The edible red alga, Gracilaria verrucosa, inhibits lipid accumulation and ROS production, but improves glucose uptake in 3T3-L1 cells. Phytother Res. 27(7):1102-5.   , Journal

Fujimori et al. 2013. Avicularin, a plant flavonoid, suppresses lipid accumulation through repression of C/EBPα-activated GLUT4-mediated glucose uptake in 3T3-L1 cells. J Agric Food Chem. 61(21):5139-47.   , Journal

DesRochers et al. 2013. Bioengineered 3D human kidney tissue, a platform for the determination of nephrotoxicity. PLoS One. 8(3):e59219.   , Journal

Monden et al. 2013. Receptor for advanced glycation end products regulates adipocyte hypertrophy and insulin sensitivity in mice: involvement of Toll-like receptor 2. Diabetes. 62(2):478-89.   , Journal

Ishikawa-Kobayashi et al. 2012. Reduced histone H3K9 acetylation of clock genes and abnormal glucose metabolism in ob/ob mice. Chronobiol Int. 29(8):982-93.   , Journal