2) PCR Related Reagents
A Treasure Box of Tools for Molecular Biology
Products on this page (PCR Related Items)
Anti-Taq high Anti-Taq DNA polymerase antibody
dNTPs Mixture(2mM)
dNTPs Mixture(10mM)
RNase inhibitor
TArget Clone™ High efficient TA cloning kit
TArget Clone™ -Plus- High efficient TA cloning kit for KOD products
10xA-attachment mix dA attachment reagent
PCR RELATED PRODUCTS
Anti-Taq DNA polymerase antibody
Anti-Taq high
This product is a highly purified neutralization monoclonal antibody to Taq DNA polymerase. The product can be applied for hot start PCR using Taq DNA polymerase by mixing with the Taq DNA polymerase solution.
Cat. No. TYB-TCP-101
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dNTPs Mixture (2mM or 10mM)
Cat. No. TYB-NTP-201 2 µmoles/1 mL (2 mM)Cat. No. TYB-NTP-301 2 µmoles/0.2 mL (10 mM)
Suitable for PCR and reverse transcription
dNTPs Mixture is an equilmolar solution mixture of ultrapure dATP, dCTP, dGTP and dTTP.
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RNase inhibitor
This product is a recombinant type human ribonuclease inhibitor (RNase inhibitor; 51kDa) that is produced by E. coli. RNase inhibitor forms a complex with ribonuclease A (RNase A) and inhibits the activity of RNase A. This inhibitor can be applied to reverse transcriptase reactions.
Cat. No. TYB-SIN-201
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high efficient TA cloning kit
TArget Clone™
Ligation step can be completed in 5 min with high efficiency.
PCR products can be used without purification.
TArget Clone™ [Code No. TAK-101] can be applied to the TA cloning of PCR products amplified using Taq DNA polymerase, Blend Taq™ [Cat No. BTQ-101], Blend Taq™ -Plus- [Cat. No. BTQ-201] or KOD Dash [Cat. No. LDP-101]. Almost all PCR products having dA overhanging at the 3'end are available.
Cat No. TYB-TAK-101
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high efficient TA cloning kit for KOD products
TArget Clone™ -Plus-
Can be applied to the TA cloning of blunt-end PCR products of KOD DNA polymerase.
Ligation step can be completed in 5 min with high efficiency.
PCR products can be used without purification.
TArget Clone™ -Plus- [Code No. TAK-201] can be applied to the TA cloning of blunt-end PCR products amplified using KOD -Plus- [Code No. KOD-201, KOD-401] or KOD FX [Code No. KFX-101, KFX-201]. TArget Clone™ -Plus- contains 10xA-attachment mix. This reagent is a mixture of anti-KOD DNA polymerase antibody specific to KOD 3'?5' exonuclease activity (proof-reading activity) and Taq DNA polymerase, which exhibits terminal transferase activity. The 10 x A-attachment mix allows for blunt end PCR products to acquire overhanging dA at the 3'-ends.
Cat. No. TYB-TAK-201
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dA attachment reagent
10xA-Attachment Mix
The attachment reaction is completed in 10 minutes at 60ºC.
The cloning efficiency of dA attached PCR products is as great as that of PCR products of Taq DNA polymerase.
10 x A-attachment mix is a reagent consists of the anti-KOD DNA polymerase antibody for the 3'?5' exonuclease activity (proof-reading activity) of the enzyme, and Taq DNA polymerase exhibits the terminal transferase activity. The PCR products from KOD -Plus- [Code No. KOD-201] and KOD FX [Code No. KFX-101] have blunt ends due to the 3'?5' exonuclease activity of KOD DNA polymerase. 10 x A-attachment mix allows the PCR products to acquire overhanging dA at the 3'-ends. The products having 3'-dA overhang can be directly cloned into arbitrary T-vectors using ligation reagents such as Ligation high Ver.2 [Code No. LGK-201].
Cat. No. TYB-TAK-301
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