Mebiol (MBG) Application Examples

1) Culture of primary cancer cells in Mebiol Gel

Selective growth of only primary cancer cells from human cancerous tissue in Mebiol Gel (courtesy Dr. S. Kubota, Dept. of General Surgery, St. Marianna University School of Medicine). This technology enables the characterization of patient-derived primary cancer cells and therefore enabling the evaluation of primary cells for chemosensitivity, malignancy, metastasis activity and other parameters that might influence patient therapy.

 

 Human colon cancerous tissue was cultured in Mebiol Gel for 10 days.Only primary cancer cells proliferate from the tissue in Mebiol Gel. Fibroblasts growth in Mebiol Gel is suppresed whereas In collagen and many other 3D gel culture matrices, fibroblasts overgrow and prevent proliferation of primary cancer cells.

2) Stem Cell Culture

3D culture of undifferentiated mouse and Macaca ES cells cultured without LIF or feeder layer cells performed in collaboration with with Dr. K. Hishikawa, Dept. of Clinical Renal Regeneration, University of Tokyo

 

The strong positive alkaline phosphatase staining of Macaca (primate) ES cells cultured in Mebiol Gel suggests undifferentiation. 

The strong positive alkaline phosphatase staining of Mouse ES cells cultured in Mebiol Gel suggests undifferentiation.