Bio Academia (BAM) cDNA Libraries 

  cDNA libraries

cDNA Library (Human 293T cells)
cDNA Library (Human contact inhibition state)
cDNA Library (Human HeLa cells #1)
cDNA Library (Human umbilical vein endothelial cells)
cDNA Library (Mouse testis)
cDNA Library (Mouse thymocytes)
cDNA Library (Planaria)
cDNA Library (Rat embryonic fibroblast)
cDNA Library (Rat NRK cell log phase)
cDNA Library (S. cerevisiae, Log Phase)
cDNA Library (S. pombe h-L972, after HU, gamma irradiation and MMS treatment)
cDNA Library (S. pombe, Meiosis)
cDNA Library (S. pombe, Mitosis)
cDNA Library (Xenopus oocytes)

 

Human

BAM-02-721-EX          cDNA Library, Human ; Umbilical Vein Endothelial Cells
This cDNA library (plasmid DNA) is constructed from Human umbilical vein endothelial cell (HUVEC)-derived poly(A)+ RNA by the Linker-Primer method by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of NotI and BamHI (BglII)-SmaI adaptor. The pAP3neo vector used in this library can express human genes in mammalian cells as it contains SV40 promoter. It also contains the Ori of pUC plasmid required for replication in E. coli, f1 ori which is necessary for ssDNA synthesis, and bacteriophage T7 and T3 promoters for RNA synthesis. GenBank Accession No. AB003468

BAM-02-723-EX          cDNA Library, Human ; HeLa (#1)
This cDNA library (plasmid DNA) is constructed from HeLa cell-derived poly(A)+ RNA by the Linker-Primer method by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of NotI and BamHI (BglII)-SmaI adaptor. The pAP3neo vector used in this library can express human genes in mammalian cells as it contains an SV40 promoter. It also contains the Ori of pUC plasmid required for replication in E.coli, f1 ori which is necessary for ssDNA synthesis, and bacteriophage T7 and T3 promoters for RNA synthesis. GenBank Accession No. AB003468

BAM-02-727-EX          cDNA Library, Human contact inhibition state
This cDNA library (plasmid DNA) is constructed from poly(A)+ RNA of human TIG-1 cells (fetal lung fibroblasts) at contact inhibition state. It is constructed by the Linker-Primer method by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of NotI, and BamHI (BglII)-SmaI adaptor. The pAP3neo vector used in this library can express human genes in mammalian cells as it contains an SV40 promoter. It also contains the Ori of pUC plasmid required for replication in E.coli, f1 ori which is necessary for ssDNA synthesis, and bacteriophage T7 and T3 promoters for RNA synthesis. GenBank Accession No. AB003468

BAM-02-729-EX          cDNA Library, Human ; 293T
This cDNA library (plasmid DNA) is constructed from human embryonic kidney 293T cell-derived poly(A)+ RNA by the Linker-Primer method by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of NotI and BamHI (BglII)-Sma I adaptor. The pAP3neo vector used in this library can express human genes in mammalian cells as it contains an SV40 promoter. It also contains the Ori of pUC plasmid required for replication in E.coli, f1 ori which is necessary for ssDNA synthesis, and bacteriophage T7 and T3 promoters for RNA synthesis. GenBank Accession No. AB003468

Rat

BAM-02-717-EX          cDNA Library, Rat ; Rat embryonic fibroblast
This cDNA library (plasmid DNA) is constructed from rat embryonic fibroblast-derived poly(A)+ RNA by the Linker-Primer method by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of NotI and BamHI (BglII)-SmaI adaptor. The pAP3neo vector used in this library can express rat genes in mammalian cells as it contains an SV40 promoter. It also contains the Ori of pUC plasmid required for replication in E. coli, f1 ori which is necessary for ssDNA synthesis, and bacteriophage T7 and T3 promoters for RNA synthesis. GenBank Accession No. AB003468

BAM-02-719-EX          cDNA Library, Rat NRK Cell Log Phase
This cDNA library (plasmid DNA) is constructed from poly(A)+ RNA of rat NRK (normal rat kidney) cells at the 0hr log phase. It is constructed by the Linker-Primer method by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (BglII)-SmaI adaptor. The pAP3neo vector used in this library can express rat genes in mammalian cells as it contains an SV40 promoter. It also contains the Ori of pUC plasmid required for replication in E.coli, f1 ori which is necessary for ssDNA synthesis, and bacteriophage T7 and T3 promoters for RNA synthesis. GenBank AccessionNo. AB003468

Mouse

BAM-02-713-EX          cDNA Library, Mouse ; Thymocyte
This cDNA library (plasmid DNA) is constructed from mouse thymocyte-derived poly(A)+ RNA by the Linker-Primer method by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of NotI and BamHI (BglII)-SmaI adaptor. The pAP3neo vector used in this library can express genes in mammalian cells as it contains an SV40 promoter. It also contains f1 ori which is necessary for ssDNA synthesis, and bacteriophage T7 and T3 promoters for RNA synthesis. GenBank Accession No. AB003468

02-715-EX          cDNA Library, Mouse ; Testis
This cDNA library (plasmid DNA) is constructed from mouse testis-derived poly(A)+ RNA by the Linker-Primer method by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of NotI and BamHI (BglII)-SmaI adaptor. The pAP3neo vector used in this library can express mouse genes in mammalian cells as it contains an SV40 promoter. It also contains a pUC plasmid Ori required for replication in E. coli and f1 ori which is necessary for ssDNA synthesis, and bacteriophage T7 and T3 promoters for RNA synthesis. GenBank Accession No. AB003468

Xenopus

BAM- 02-711-EX          cDNA Library, Xenopus ; oocyte
This cDNA library (plasmid DNA) is constructed from Xenopus oocyte-derived poly(A)+ RNA by the Linker-Primer method by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (BglII)-SmaI adaptor. The pBA2 vector used in this library has the pUC ori which enables replication in E. coli and Ampr as a selection marker.

Planaria

 BAM-02-709-EX          cDNA Library, Planaria ; Planaria
This cDNA library (plasmid DNA) is constructed from Planaria-derived poly(A)+ RNA by the Linker-Primer method by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of NotI, and BamHI (BglII)-SmaI adaptor. The pAP3neo vector used in this library can express genes in mammalian cells as it contains an SV40 promoter. It also contains the Ori for plasmid replication and f1 ori which is necessary for ssDNA synthesis in E. coli, and bacteriophage T7 and T3 promoters for RNA synthesis. GenBank Accession No. AB003468

S.pombe

BAM-02-701-EX          cDNA Library, S.cerevisiae Log Phase
This cDNA library (plasmid DNA) is constructed from Saccharomyces cerevisiae, strain S288C-derived poly(A)+ RNA at the log phase by the Linker-Primer method by Prof. H. Nojima of Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of NotI and BamHI (BglII)-SmaI adaptor. The pLZ3 vector used in this library cannot replicate in S. cerevisiae but contains the pUC ori for replication in E. coli.

BAM-02-703-EX          cDNA Library, S.pombe ; mitosis
This cDNA library (plasmid DNA) is constructed from Schizosaccharomyces pombe strain h-L972-derived poly(A)+ RNA at the log phase by the Linker-Primer method by Prof. H. Nojima of Research Institute for Microbial Diseases, Osaka University. cDNAs in this library are unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme sites of NotI and BamHI (BglII)-SmaI adaptor. The pLZ3 vector used in this library can replicate both in S. pombe and E. coli and express S. pombe genes in mammalian cells as it contains an SV40 promoter.

BAM-02-705-EX          cDNA Library, S.pombe ; Meiosis
This cDNA library (plasmid DNA) is constructed from Schizosaccharomyces pombe, strain CD16-1(h+/h-) derived poly(A)+ RNA at the state of miosis by the Linker-Primer method by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka University. cDNAs in this library are unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of NotI and BamHI (BglII)-SmaI adaptor. The pAP3neo vector used in this library can express S. pombe genes in mammalian cells as it contains an SV40 promoter. It also contains an f1 ori which is necessary for ssDNA synthesis, and bacteriophage T7 and T3 promoters for RNA synthesis. GenBank Accession No. AB003468

BAM-02-707-EX          cDNA Library, S.pombe ; After + HU, ?, MMS h-L972
This cDNA library (plasmid DNA) is constructed from poly(A)+ RNA derived from Schizosaccharomyces pombe (strain h-L972) after treatment with HU (hydroxyurea), gamma-radiation, and MMS (methylmethane sulfonate). It is constructed by the Linker-Primer method by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of NotI and BamHI (BglII)-SmaI adaptor. The pLZ3 vector used in this library can replicate both in S. pombe and in E. coli, and express cloned genes not only in S. pombe but also in mammalian cells as it contains an SV40 promoter. It also contains an f1 ori which is necessary for ssDNA synthesis, and bacteriophage T7 and T3 promoters for RNA synthesis.