Afamin/Wnt3a CM (Conditioned Medium)

MBL Life Sciences

Catalog No.: MBL-J2-001

Optimized Wnt3a Activity for Reliable Organoid and Stem Cell Culture

Afamin/Wnt3a CM is a high-performance, serum-free conditioned medium designed to enhance the stability and biological activity of Wnt3a—a critical factor for organoid formation and Lgr5+ stem cell maintenance.

Unlike standard Wnt3a, which is prone to aggregation in defined media, this formulation features a co-expressed complex of mouse Wnt3a and human Afamin, a vitamin E-binding glycoprotein that dramatically improves Wnt3a solubility and bioavailability. The result is consistent, long-term Wnt signaling ideal for culturing organoids from the intestine, stomach, pancreas, and liver.

Key Benefits

• Enhanced Solubility & Stability – Afamin-Wnt3a complex ensures sustained Wnt3a bioactivity
• Serum-Free & Defined – Reduces variability for reproducible organoid culture
• Supports Lgr5+ Stem Cells – Promotes self-renewal and long-term growth
• Ready to Use – Sterile, endotoxin-tested (<1.0 EU/mL), and filtered (0.2μm)
• High-Yield Expression – Produced using Expi293F™ cells with PA- and TARGET-tagged constructs

Applications

• Intestinal, gastric, hepatic, and pancreatic organoid culture
• Stem cell maintenance
• Canonical Wnt signaling activation in defined systems

Usage Instructions

Use at a final concentration of 10% (v/v) in culture media. Optimize as needed for specific cell types or experimental designs.

Storage & Handling

Store at –20°C or lower. Avoid repeated freeze–thaw cycles—aliquot upon first thaw.

Note: This product may be subject to third-party patent rights. Please consult your institution’s legal team for guidance.

References

1. Mihara, E., Matano, M., Yamamoto, H., Sato, T., & Takagi, J. (2016). Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein Afamin/α-albumin. eLife, 5, e11621. [PMID: 26902720]
2. Nanki, K., Toshimitsu, K., Takano, A., Fujii, M., & Sato, T. (2018). Divergent routes toward Wnt and R-spondin niche independency during human gastric carcinogenesis. Cell, 174(4), 856–869.e17. [PMID: 30096312]
3. Sugimoto, S., Ohta, Y., Fujii, M., et al. (2018). Reconstruction of the human colon epithelium in vivo. Cell Stem Cell, 22(2)

Product Specifications

• Application: Cell Culture
• Endotoxin Levels: < 1.0 EU/mL by the LAL assay