Afamin/Wnt3a CM (Conditioned Medium)

MBL Life Sciences

Catalog No.: MBL-J2-001

Product Description

Serum free conditioned medium including Wnt3a-Afamin complex for intestinal organoid cell culture. Note Regarding Intellectual Property Rights: When culturing organoids, or stem cells, or other tissues, if you are to use this product in combination with other factor or factors (hereunder factors), a third party may have a patent on the use or other application of the factors concerned. Regarding to this product, we do not offer any non-infringement warranty when used or otherwise applied in combination with other factors. Therefore, if you intend to use this product in combination with other factors, please check with your organization’s division responsible for intellectual property rights or your research agency before using this product. Conditioned medium containing Wnt3a protein and Afamin protein. The mouse Wnt3a and the human Afamin were co-expressed in Expi293F™ Cells*. For the co-expression of Wnt3a and Afamin, expression plasmids for N-terminal PA tagged mouse wnt3a and N-terminal TARGET tagged human afamin were co-transfected using the Expi293 Expression System*. Conditioned medium was prepared using serum-free media (Advanced DMEM/F-12* containing streptomycin, HEPES and GlutaMAXTM Supplement*). Then, it was sterilized by filtration of 0.2 µm-filter. FBS was used for pre-culture of Afamin/Wnt3a expression Expi293F™ Cells. *: Thermo Fisher Scientific’s products.

DIRECTION for USE:

As a canonical Wnt signal agonist. For intestinal organoids culture, add to the culture media at a final concentration of 10%. Since the optimal condition may vary depending on samples, each user should determine the optimal volume of addition in advance. Formerly Product Code: J-ORMW301R

Product Specifications

• Application: Cell Culture
• Endotoxin Levels: < 1.0 EU/mL by the LAL assay