state-of-the-art in vitro protein synthesis by

"Up to 100's of micrograms/ml in 240 minutes"

 Technical Notes, Posters, Citations

go to: PUREfrex Dashboard
PUREfrex® Ordering Information Table

Annoucements
Posted 12 Janunary 2022

 

Year Type Title Authors, Link
 2022  Citation

 "Nascent peptide-induced translation discontinuation in eukaryotes impacts biased amino acid usage in proteomes." bioRxiv (2022). 

 Ito, Yosuke, et al. https://www.biorxiv.org/content/10.1101/2022.01.27.477990v1.abstract
 2022  Citation

 "Mechanistic insights into intramembrane proteolysis by E. coli site-2 protease homolog RseP." bioRxiv (2022).

 Imaizumi, Yuki, et al.  https://www.biorxiv.org/content/biorxiv/early/2022/01/31/2022.01.31.478169.full.pdf
 2022  Citation

 "Synthetic minimal cells and their applications." New Frontiers and Applications of Synthetic Biology. Academic Press, 2022. 83-101. 

 Kharrazi, Ali Yazdizadeh, et al.https://www.sciencedirect.com/science/article/pii/B9780128244692000300
 2022  Citation

"Transfer RNA synthesis-coupled translation and DNA replication in a reconstituted." 

 Miyachi, Ryota, Yoshihiro Shimizu, and Norikazu Ichihashi. https://pubmed.ncbi.nlm.nih.gov/35848947/
 2022  Citation

 "The landscape of translational stall sites in bacteria revealed by monosome and disome profiling." RNA 28.3 (2022): 290-302. 

 Fujita, Tomoya, et al.https://pubmed.ncbi.nlm.nih.gov/34906996/
 2022  Citation

"Modern and prebiotic amino acids support distinct structural profiles in proteins." Open Biology 12.6 (2022): 220040. 

 Tretyachenko, Vyacheslav, et al. https://pubmed.ncbi.nlm.nih.gov/35728622/
 2022  Citation

 "The Escherichia coli DnaK chaperone stimulates the a-complementation of ß-galactosidase." Journal of Basic Microbiology (2022). 

 Berhanu, Samuel, Takuya Ueda, and Jean-Hervé Alix.https://onlinelibrary.wiley.com/doi/abs/10.1002/jobm.202100487
 2022  Citation

 "In vitro assembly, positioning and contraction of a division ring in minimal cells." (2022). 

 Kohyama, Shunshi, Adrian Merino-Salomon, and Petra Schwille.https://www.researchsquare.com/article/rs-1284300/latest.pdf
 2022  Citation

Development of electrochemical biosensors using insect odorant receptors. Diss. ResearchSpace@ Auckland, 2022. 

 Cheema, Jamal Ahmed. https://researchspace.auckland.ac.nz/bitstream/handle/2292/58253/Cheema-2022-thesis.pdf?sequence=4
 2022  Citation

 "In vitro transcription/translation-coupled DNA replication through the regeneration of 20 aminoacyl-tRNA synthetases." 

 Hagino, Katsumi, and Norikazu Ichihashi. https://jxiv.jst.go.jp/index.php/jxiv/preprint/download/53/497
 2022  Citation

"Mode selection mechanism in traveling and standing waves revealed by Min wave reconstituted in artificial cells." Science Advances 8.23 (2022): eabm8460. 

 Takada, Sakura, et al. https://pubmed.ncbi.nlm.nih.gov/35675408/
 2022  Citation

"Measurement of Transcription, Translation, and Other Enzymatic Processes During Cell-Free Expression Using PERSIA." Cell-Free Gene Expression. Humana, New York, NY, 2022. 169-181. 

 Wick, Scott, and Peter A. Carr. https://pubmed.ncbi.nlm.nih.gov/34985744/
 2022  Citation

"Photoinducible Azobenzene trimethylammonium bromide (AzoTAB)-mediated giant vesicle fusion compatible with synthetic protein translation reactions." Biochemical and Biophysical Research Communications 618 (2022): 113-118. 

 Xu, Boying, et al. https://pubmed.ncbi.nlm.nih.gov/35717905/
2022 Citation

 "High-throughput screening of cell-free riboswitches by fluorescence-activated droplet sorting." Nucleic acids research 50.6 (2022): 3535-3550.

 Tabuchi, Takeshi, and Yohei Yokobayashi.  https://pubmed.ncbi.nlm.nih.gov/35253887/
2022 Citation

 "In vitro reconstitution of the Escherichia coli 70S ribosome with a full set of recombinant ribosomal proteins." J. Biochem. 171.2 (2022): 227-237. 

 Aoyama, Ryo, et al. https://pubmed.ncbi.nlm.nih.gov/34750629/
2022 Citation

 "Cell-Free Mutant Analysis Combined with Structure Prediction of a Lasso Peptide Biosynthetic Protease B2." ACS Synthetic Biology (2022). 

 Alfi, Almasul, et al.https://pubmed.ncbi.nlm.nih.gov/35674818/
2022 Citation

"Embedding a membrane protein into an enveloped artificial viral replica." RSC chemical biology 3.2 (2022): 231-241. 

 Furukawa, Hiroto, et al. https://pubmed.ncbi.nlm.nih.gov/35360888/
2022 Citation

"Formation and function of OmpG or OmpA-incorporated liposomes using an in vitro translation system." Scientific reports 12.1 (2022): 1-7. 

 Kamiya, Koki. https://pubmed.ncbi.nlm.nih.gov/35149747/
2022 Citation

 "Identification of conditions for efficient cell-sized liposome preparation using commercially available reconstituted in vitro transcription-translation system." Journal of Bioscience and Bioengineering 133.2 (2022): 181-186. 

 Uyeda, Atsuko, et al.https://pubmed.ncbi.nlm.nih.gov/34789414/
2022 Citation

"Chip assisted formation of phase-separated liposomes for reconstituting spatial protein–lipid interactions." Lab on a Chip (2022). 

 Chien, Po-Jen, et al. https://pubmed.ncbi.nlm.nih.gov/35667105/
2021 Citation Chemical Synthesis and Cell-Free Expression of Thiazoline Ring-Bridged Cyclic Peptides and Their Properties on Biomembrane Permeability Takashi Tamura, Masaaki Inoue, Yuji Yoshimitsu, Ichihiko Hashimoto, Noriyuki Ohashi, Kyosuke Tsumura, Koo Suzuki, Takayoshi Watanabe and Takahiro Hohsaka.
Bull Chem Soc Jpn. 2022;95(2):359-366.
https://doi.org/10.1246/bcsj.20210409
2021 Citation Cell-Free Synthesis of Human Endothelin Receptors and Its Application to Ribosome Display Hiroki Nakai, Kinuka Isshiki, Masato Hattori, Hiromasa Maehira, Tatsumi Yamaguchi, Keiko Masuda, Yoshihiro Shimizu, Takayoshi Watanabe, Takahiro Hohsaka, Wataru Shihoya, Osamu Nureki, Yasuhiko Kato, Hajime Watanabe and  Tomoaki Matsuura.
Anal Chem. 2022 Mar 8;94(9):3831-3839.
https://doi.org/10.1021/acs.analchem.1c04714​
2021 Citation Formation and function of OmpG or OmpA-incorporated liposomes using an in vitro translation system Koki Kamiya.
Sci Rep. 2022 Feb 11;12(1):2376.
https://doi.org/10.1038/s41598-022-06314-4
2021 Citation Efficient and Precise Protein Synthesis in a Cell-Free System Using a Set of In Vitro Transcribed tRNAs with Nucleotide Modifications Kazuaki Amikura, Keita Hibi and Yoshihiro Shimizu.
Methods Mol Biol. 2022;2433:151-168.
https://doi.org/10.1007/978-1-0716-1998-8_9
2021 Citation Embedding a membrane protein into an enveloped artificial viral replica Hiroto Furukawa, Hiroshi Inaba, Yoshihiro Sasaki, Kazunari Akiyoshic  and  Kazunori Matsuura.
RSC Chem Biol. 2021 Dec 21;3(2):231-241.
https://doi.org/10.1039/D1CB00166C
2021 Citation Identification of conditions for efficient cell-sized liposome preparation using commercially available reconstituted in vitro transcription-translation system Atsuko Uyeda, Sabrina Galiñanes Reyes, Takashi Kanamori and Tomoaki Matsuura.
J Biosci Bioeng. 2021 Nov 14;S1389-1723(21)00290-5.
https://doi.org/10.1016/j.jbiosc.2021.10.008
2021 Citation In vitro reconstitution of the Escherichia coli 70S ribosome with a full set of recombinant ribosomal proteins Ryo Aoyama, Keiko Masuda, Masaru Shimojo, Takashi Kanamori, Takuya Ueda and Yoshihiro Shimizu.
J Biochem. 2022 Feb 21;171(2):227-237.
https://doi.org/10.1093/jb/mvab121
2021 Citation Structure-activity relationship for the folding intermediate-selective inhibition of DYRK1A Yuka Miyazaki, Masaki Kikuchi, Koji Umezawa, Aurelie Descamps, Daichi Nakamura, Gaku Furuie, Tomoe Sumida, Kanako Saito, Ninako Kimura, Takashi Niwa, Yuto Sumid, Takashi Umehara, Takamitsu Hosoya and Isao Kii.
Eur J Med Chem. 2022 Jan 5;227:113948.
Epub 2021 Oct 28.
https://doi.org/10.1016/j.ejmech.2021.113948
2021 Citation Nascent polypeptide within the exit tunnel stabilizes the ribosome to counteract risky translation Yuhei Chadani, Nobuyuki Sugata, Tatsuya Niwa, Yosuke Ito, Shintaro Iwasaki and Hideki Taguchi.
EMBO J. 2021 Dec 1;40(23):e108299.
https://doi.org/10.15252/embj.2021108299
2021 Citation Insect odorant receptor-based biosensors: Current status and prospects Jamal Ahmed Cheema, Colm Carraher, Natalie O V Plank, Jadranka Travas-Sejdic and Andrew Kralicek.
Biotechnol Adv. 2021 Oct 1;53:107840.
https://doi.org/10.1016/j.biotechadv.2021.107840​
2021 Citation Shaping Liposomes by Cell-Free Expressed Bacterial Microtubules Johannes Kattan, Anne Doerr, Marileen Dogterom and Christophe Danelon.
ACS Synth Biol. 2021 Oct 15;10(10):2447-2455.
https://doi.org/10.1021/acssynbio.1c00278
2021 Citation Discovery of a small protein-encoding cis-regulatory overlapping gene of the tumor suppressor gene Scribble in humans Yuhta Nomura and Naoshi Dohmae.
Commun Biol. 2021 Sep 17;4(1):1098.
https://doi.org/10.1038/s42003-021-02619-8
2021 Citation Cell-free riboswitches Takeshi Tabuchi and Yohei Yokobayashi.
RSC Chem Biol. 2021 Aug 4;2(5):1430-1440.
https://doi.org/10.1039/D1CB00138H
2021 Citation A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infection Tingting Li, Hongmin Cai, ... and Dianfan Li.
Nat Commun. 2021 Jul 30;12(1):4635.
https://doi.org/10.1038/s41467-021-24905-z​
2021 Citation Cell free protein synthesis versus yeast expression - a comparison using insulin as a model protein Astrid B Jensen, Franta Hubálek, Carsten Enggaard Stidsen, Eva Johansson, Fredrik Kryh Öberg, Michael Skjøt and Thomas Kjeldsen. Protein Expr Purif. 2021 Oct;186:105910.
https://doi.org/10.1016/j.pep.2021.105910​
2021 tech note Synthesis of Protein Toxin (Gelonin) using DnaK Mix Gelonin is a 30 kDa potein toxin from plant Gelonium multiflorum. It is know as one of the ribosome-inactivating protein (RIP) and has rRNA N-glyco...
2021 tech note 6 Tips about the template DNA Design Certain nucleotide or amino acid sequence may reduce the yield of the protein of interest. In such cases, optimizing the sequence may improve the y...
2020 tech note Preparation of the template DNA The design and preparation of template DNA for PUCitationrex® is a very important step for successful protein synthesis. The following is a summary of t...
2020 tech note Sequence of DHFR DNA DHFR DNA in the PUCitationrex® kit is a PCR product containing a gene encoding dihydrofolate reductase (DHFR) from E.coli (18 kDa). The text shown belo...
2021 Posters Unique cell-free protein synthesis system, PUCitationrex – Useful platform for protein expression in the development of biologics - Abstruct PUCitationrex® is a cell-free synthesis system based on PURE (Protein synthesis Using Recombinant Element) system. PURE system is a reconstitut...
2018 Posters Improvement of the translation efficiency of proline residue-containing proteins using the reconstituted cell-free protein synthesis sytem (PUCitationrex) Abstract PUCitationrex® is a build up cell-free protein synthesis system (PURE system) that reconstitutes the factors involved in transcription and tran...
2018 Posters Synthesis of proteins containing disulfide bonds using PUCitationrex with human protein disulfide isomerase Abstruct The PURE system is a cell-free protein synthesis system reconstituted from only factors involved in protein synthesis in E. coli. Here, we...
2021 Citation Boundary-Free Ribosome Compartmentalization by Gene Expression on a Surface Michael Levy, Reuven Falkovich, Ohad Vonshak, Dan Bracha, Alexandra M Tayar, Yoshihiro Shimizu, Shirley S Daubev and Roy H Bar-Ziv. ACS Synth Biol....
2021 Citation Cell-Free Expression of a Plant Membrane Protein BrPT2 From Boesenbergia Rotunda Yvonne Jing Mei Liew, Yean Kee Lee, Norzulaani Khalid, Noorsaadah Abd Rahman and Boon Chin Tan. Mol Biotechnol. 2021 Apr;63(4):316-326. https://doi...
2021 Citation Search for translation arrest peptides encoded upstream of genes for components of protein localization pathways Karen Sakiyama, Naomi Shimokawa-Chiba, Keigo Fujiwara and Shinobu Chiba. Nucleic Acids Res. 2021 Feb 22;49(3):1550-1566. https://doi.org/10.1093/na...
2021 Citation PURE mRNA display and cDNA display provide rapid detection of core epitope motif via high-throughput sequencing Sabrina Galiñanes Reyes, Yutetsu Kuruma, Mai Fujimi, Masako Yamazaki, Sumie Eto, Shota Nishikawa, Satoshi Tamaki, Asaki Kobayashi, Ryo Mizuuchi, Ly...
2021 Citation Highly sensitive VOC detectors using insect olfactory receptors reconstituted into lipid bilayers Tetsuya Yamada, Hirotaka Sugiura, Hisatoshi Mimura, Koki Kamiya, Toshihisa Osaki and Shoji Takeuchi. Sci Adv. 2021 Jan 13;7(3):eabd2013. https://do...
       
2020 Citation Reconstitution of mammalian mitochondrial translation system capable of correct initiation and long polypeptide synthesis from leaderless mRNA Muhoon Lee, Noriko Matsunaga, Shiori Akabane, Ippei Yasuda, Takuya Ueda and Nono Takeuchi-Tomita. Nucleic Acids Res. 2021 Jan 11;49(1):371-382. htt...
2020 Citation Proteome-wide Capture of Co-translational Protein Dynamics in Bacillus subtilis Using TnDR, a Transposable Protein-Dynamics Reporter Keigo Fujiwara, Yutaro Katagi, Koreaki Ito and Shinobu Chiba. Cell Rep. 2020 Oct 13;33(2):108250. https://doi.org/10.1016/j.celrep.2020.108250
2020 Citation CoLiDe: Combinatorial Library Design tool for probing protein sequence space Vyacheslav Tretyachenko, Václav Voráček, Radko Souček, Kosuke Fujishima and Klára Hlouchová. Bioinformatics. 2020 Sep 21;btaa804. Online ahead of p...
2020 Citation Enzymatic Regulation and Biological Functions of Reactive Cysteine Persulfides and Polysulfides Tomohiro Sawa , Hozumi Motohashi , Hideshi Ihara and Takaaki Akaike Biomolecules 2020, 10(9), 1245. https://doi.org/10.3390/biom10091245​
2020 Citation Reconstituted Cell-Free Protein Synthesis Using in Vitro Transcribed tRNAs Keita Hibi, Kazuaki Amikura, Naoki Sugiura, Keiko Masuda, Satoshi Ohno, Takashi Yokogawa, Takuya Ueda and Yoshihiro Shimizu. Commun Biol. 2020 Jul ...
2020 Citation Ribosome Rescue Activity of an Arabidopsis Thaliana ArfB Homolog Michiaki Nagao, Fumina Tsuchiya, Reiko Motohashi and Tatsuhiko Abo. Genes Genet Syst. 2020 Aug 27;95(3):119-131. https://doi.org/10.1266/ggs.20-00...
2020 Citation YkgM and YkgO Maintain Translation by Replacing Their Paralogs, Zinc-Binding Ribosomal Proteins L31 and L36, With Identical Activities Masami Ueta, Chieko Wada and Akira Wada. Genes Cells 2020 Aug; 25(8): 562-581. https://doi.org/10.1111/gtc.12796
2020 Citation In Vitro Evolution of phi29 DNA Polymerases Through Compartmentalized Gene Expression and Rolling-Circle Replication Yoshihiro Sakatani, Ryo Mizuuchi and Norikazu Ichihashi Protein Eng Des Sel. 2019 Dec 31;32(11):481-487. https://doi.org/10.1093/protein/gzaa011
2020 Citation The roles of histidine and tyrosine residues in the active site of collagenase in Grimontia hollisae. Hayashi K, Ikeuchi T, Morishita R, Qian J, Kojima K, Takita T, Tanaka K, Hattori S and Yasukawa K. J Biochem. 2020 Oct 1;168(4):385-392. https://do...
2020 Citation Development of a monoclonal antibody for specific detection of Vibrio parahaemolyticus and analysis of its antigen. Taro Yonekita, Naoki Morishita, Eiji Arakawa and Takashi Matsumoto. J Microbiol Methods 2020 Apr 11;105919. https://doi.org/10.1016/j.mimet.2020.10...
2020 Citation In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli. Shimojo M, Amikura K, Masuda K, Kanamori T, Ueda T and Shimizu Y. Commun Biol. 2020 Mar 25;3(1):142. https://doi.org/10.1038/s42003-020-0874-8
2020 Citation The mechanism of action of Spi-B in the transcriptional activation of the interferon-α4 gene. Miyazaki R, Saiga H, Kato T, Bakoshi T, Senba R, Shintani A, Suzuki M, Takao K, Sasaki I, Iizuka A, Sugiyama M, Iwami N, Fukuda-Ohta Y, Hemmi H, Ta...
2020 Citation Stem cell development involves divergent thyroid hormone receptor subtype expression and epigenetic modifications in the Xenopus metamorphosing intestine. Hasebe T, Fujimoto K, Buchholz DR and Ishizuya-Oka A. Gen Comp Endocrinol. 292:113441. [Epub ahead of print] https://doi.org/10.1016/j.ygcen.2020....
2020 Citation Nascent SecM chain interacts with outer ribosomal surface to stabilize translation arrest. Muta M, Iizuka R, Niwa T, Guo Y, Taguchi H and Funatsu T. Biochem J. 2020 Jan 31;477(2):557-566. https://doi.org/10.1042/BCJ20190723
2020 Citation Microfluidic screening system based on boron-doped diamond electrodes and dielectrophoretic sorting for directed evolution of NAD(P)-dependent oxidoreductases. Goto H, Kanai Y, Yotsui A, Shimokihara S, Shitara S, Oyobiki R, Fujiwara K, Watanabe T, Einaga Y, Matsumoto Y, Miki N and Doi N. Lab Chip. 2020 Feb...
2019 Citation Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens. Su J, Kitaguchi T, Ohmuro-Matsuyama Y, Seah T, Ghadessy FJ, Hoon S and Ueda H. Sci Rep. 9:18189 https://doi.org/10.1038/s41598-019-54539-7
2019 Citation Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis. Shimokawa-Chiba N, Müller C, Fujiwara K, Beckert B, Ito K, Wilson DN and Chiba S. Nat Commun. 10, 5397. (2019) https://doi.org/10.1038/s41467-019-...
2019 Citation Centrifuge-based step emulsification device for simple and fast generation of monodisperse picoliter droplets Dong-Chel Shina, Yuya Morimotoab, Jun Sawayamaa, Shigenori Miuraa and Shoji Takeuchi Sensors and Actuators B: Chemical. 301, 127164. (2019) https:/...
2019 Citation Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array Yi Zhang, Yoshihiro Minagawa, Hiroto Kizoe, Kentaro Miyazaki, Ryota Iino, Hiroshi Ueno, Kazuhito V. Tabata, Yasuhiro Shimane and Hiroyuki Noji Scie...
2019 Citation Genetically Encoded Membranes for Bottom‐Up Biology Kilian Vogele, Tobias Pirzer and Friedrich C. Simmel ChemSystemsChem (2019) online version https://doi.org/10.1002/syst.201900016​
2019 Citation Programmable On-Chip Artificial Cell Producing Post-Translationally Modified Ubiquitinated Protein. Zilberzwige-Tal S, Levin A, Toprakcioglu Z, Knowles TPJ, Gazit E and Elbaz J. Small. 2019 Aug;15(31):e1901780. https://doi.org/10.1002/smll.201901...
2019 Citation Programmable Artificial Cells Using Histamine-Responsive Synthetic Riboswitch Mohammed Dwidar, Yusuke Seike, Shungo Kobori, Charles Whitaker, Tomoaki Matsuura and Yohei Yokobayashi J Am Chem Soc. 2019 Jul 17;141(28):11103-111...
2019 Citation Development of a Paper-Based Luminescence Bioassay for Therapeutic Monitoring of Aminoglycosides: a Proof-of-Concept Study Matsuura H, Ujiie K, Duyen TTM, Izutsu K, Fujiyama K, Maeda S, Harada K and Hirata K. Appl Biochem Biotechnol. 1-12. (2019) https://doi.org/10.1007...
2019 Citation Quantitative imaging of gene-expressing liposomes reveals rare favorable phenotypes. Blanken D, van Nies P and Danelon C. Phys Biol. 16, 045002. (2019) https://doi.org/10.1088/1478-3975/ab0c62
2019 Citation Artificial photosynthetic cell producing energy for protein synthesis. Berhanu S, Ueda T and Kuruma Y Nat. Commun. 10, 1325. (2019) https://doi.org/10.1038/s41467-019-09147-4
2019 Citation Construction of a Biohybrid Odorant Sensor Using Biological Olfactory Receptors Embedded into Bilayer Lipid Membrane on a Chip. Misawa N, Fujii S, Kamiya K, Osaki T, Takaku T, Takahashi Y and Takeuchi S ACS Sens. 4, 711–716. (2019) https://doi.org/10.1021/acssensors.8b01615
2019 Citation Osmolyte-Enhanced Protein Synthesis Activity of a Reconstituted Translation System. Moriizumi Y, Tabata KV, Miyoshi D and Noji H. ACS Synth Biol. 8, 557-567. (2019) https://doi.org/10.1021/acssynbio.8b00513
2019 Citation Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system. Murakami, S., Matsumoto, R., & Kanamori, T. Scientific reports 9, 671. (2019) https://doi.org/10.1038/s41598-018-36691-8
2019 Citation Modelling cell-free RNA and protein synthesis with minimal systems. Anne Doerr, Elise de Reus, Pauline van Nies, Mischa van der Haar, Katy Wei, Johannes Kattan, Aljoscha Wahl and Christophe Danelon Physical biology,...
2019 Citation A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System. Lavickova B and Maerkl SJ. ACS Synth. Biol. 8, 455-462. (2019) https://doi.org/10.1021/acssynbio.8b00427​
2019 Citation Cell-free supplement mixtures: Elucidating the history and biochemical utility of additives used to support in vitro protein synthesis in E. coli extract. Dopp, J., Tamiev, D., and Reuel, N. F. Biotechnology advances. 37, 246-258. (2019) https://doi.org/10.1016/j.biotechadv.2018.12.006
2018 Citation Investigating the Effect of Chain Connectivity on the Folding of a Beta-Sheet Protein On and Off the Ribosome. Andrew P Marsden, Jeffrey J Hollins, Charles O'Neill, Pavel Ryzhov, Sally Higson, Carolina A T F Mendonça, Tristan O Kwan, Lee Gyan Kwa, Annet...
2018 Citation The Folding Pathway of an Ig Domain is Conserved On and Off the Ribosome. Pengfei Tian, Annette Steward, Renuka Kudva, Ting Su, Patrick J Shilling, Adrian A Nickson, Jeffrey J Hollins, Roland Beckmann, Gunnar von Heijne, ...
2018 Citation G-Protein Coupled Receptor Protein Synthesis on a Lipid Bilayer Using a Reconstituted Cell-Free Protein Synthesis System. Gessesse, B., Nagaike, T., Nagata, K., Shimizu, Y., and Ueda, T. Life (Basel). 2018 Nov 2;8(4):54. ​ https://doi.org/10.3390/life8040054
2018 Citation Liquid Marbles as an Easy‐to‐Handle Compartment for Cell‐Free Synthesis and In Situ Immobilization of Recombinant Proteins. Noriho Kamiya, Yuki Ohama, Kosuke Minamihata, Rie Wakabayashi and Masahiro Goto. Biotechnol J. 2018 Dec;13(12):e1800085. https://doi.org/10.1002/b...
2018 Citation Large-Scale Preparation of Giant Vesicles by Squeezing a Lipid-Coated Marshmallow-like Silicone Gel in a Buffer. Gen Hayase and Shin-Ichiro M Nomura. Langmuir. 2018 Sep 18;34(37):11021-11026. https://doi.org/10.1021/acs.langmuir.8b01801​
2018 Citation MifM-instructed translation arrest involves nascent chain interactions with the exterior as well as the interior of the ribosome. Keigo Fujiwara, Koreaki Ito and Shinobu Chiba. Sci Rep. 2018 Jul 9;8(1):10311. https://doi.org/10.1038/s41598-018-28628-y
2018 Citation Multitasking of Hsp70 chaperone in the biogenesis of bacterial functional amyloids. Shinya Sugimoto , Ken-Ichi Arita-Morioka, Akari Terao, Kunitoshi Yamanaka, Teru Ogura and Yoshimitsu Mizunoe. Commun Biol. 2018 May 31;1:52. https...
2018 Citation Development of Visible‐Light‐Responsive RNA Scissors Based on a 10–23 DNAzyme. Yukiko Kamiya, Yu Arimura, Hideaki Ooi, Kenjiro Kato, Xing-Guo Liang and Hiroyuki Asanuma. Chembiochem. 2018 Jun 18;19(12):1305-1311. https://doi.o...
2018 Citation Synthetic single domain antibodies for the conformational trapping of membrane proteins. Iwan Zimmermann, Pascal Egloff, Cedric Aj Hutter, Fabian M Arnold, Peter Stohler, Nicolas Bocquet, Melanie N Hug, Sylwia Huber, Martin Siegrist, Li...
2018 Citation Persulfide synthases that are functionally coupled with translation mediate sulfur respiration in mammalian cells Shigemoto Fujii, Tomohiro Sawa, Hozumi Motohashi and Takaaki Akaike. Br J Pharmacol. 2019 Feb;176(4):607-615. https://doi.org/10.1111/bph.14356
2018 Citation High-throughput evaluation of T7 promoter variants using biased randomization and DNA barcoding Ryo Komura, Wataru Aoki, Keisuke Motone, Atsushi Satomura and Mitsuyoshi Ueda. PLoS One. 2018 May 7;13(5):e0196905. https://doi.org/10.1371/journa...
2018 Citation Self-replication of DNA by its encoded proteins in liposome-based synthetic cells Pauline van Nies, Ilja Westerlaken, Duco Blanken, Margarita Salas, Mario Mencía and Christophe Danelon. Nat Commun. 2018 Apr 20;9(1):1583. https:/...
2018 Citation De Novo Synthesis of Basal Bacterial Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles Takumi Furusato, Fumihiro Horie, Hideaki T Matsubayashi, Kazuaki Amikura, Yutetsu Kuruma and Takuya Ueda. ACS Synth Biol. 2018 Apr 20;7(4):953-961....
2018 Citation Cell-free synthesis of stable isotope-labeled internal standards for targeted quantitative proteomics Ryohei Narumi, Keiko Masuda, Takeshi Tomonaga, Jun Adachi, Hiroki R Ueda und Yoshihiro Shimizu. Synth Syst Biotechnol. 2018 Feb 21;3(2):97-104. ht...
2018 Citation Cotranslational protein assembly imposes evolutionary constraints on homomeric proteins Eviatar Natan, Tamaki Endoh, Liora Haim-Vilmovsky, Tilman Flock, Guilhem Chalancon, Jonathan T S Hopper, Bálint Kintses, Peter Horvath, Lejla Daruk...
2018 Citation Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency Naofumi Ito, Kaoru Katoh, Hiroko Kushige, Yutaka Saito, Terumasa Umemoto, Yu Matsuzaki, Hiroshi Kiyonari, Daiki Kobayashi, Minami Soga, Takumi Era,...
2018 Citation Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system Yuta Katano, Tongyang Li, Misato Baba, Miyo Nakamura, Masaaki Ito, Kenji Kojima, Teisuke Takita and Kiyoshi Yasukawa. Biosci Biotechnol Biochem. 20...
2018 Citation Intrinsic Ribosome Destabilization Underlies Translation and Provides an Organism with a Strategy of Environmental Sensing Yuhei Chadani, Tatsuya Niwa, Takashi Izumi, Nobuyuki Sugata, Asuteka Nagao, Tsutomu Suzuki, Shinobu Chiba, Koreaki Ito and Hideki Taguchi. Mol Cell...
2018 Citation Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics Takaaki Akaike, Tomoaki Ida, Fan-Yan Wei, Motohiro Nishida, Yoshito Kumagai, Md Morshedul Alam, Hideshi Ihara, Tomohiro Sawa, Tetsuro Matsunaga, Sh...
2018 Citation 28977654 Tyson R Shepherd, Liping Du, Josefine Liljeruhm, Samudyata, Jinfan Wang, Marcus O D Sjödin, Magnus Wetterhall, Tetsuya Yomo and Anthony C Forster. ...
2018 Citation In vivo target exploration of apidaecin based on Acquired Resistance induced by Gene Overexpression (ARGO assay) Ken'ichiro Matsumoto, Kurato Yamazaki, Shun Kawakami, Daichi Miyoshi, Toshihiko Ooi, Shigeki Hashimoto and Seiichi Taguchi. Sci Rep. 2017 Sep ...
2017 Citation Application of Distributive Conjugal DNA Transfer in Mycobacterium smegmatis To Establish a Genome-Wide Synthetic Genetic Array Julius Judd, Nathalie Boucher, Erik Van Roey, Todd A Gray and Keith M Derbyshire. J Bacteriol. 2017 Sep 19;199(20):e00410-17. https://doi.org/10.1...
2017 Citation Ribosomal protein L31 in Escherichia coli contributes to ribosome subunit association and translation, whereas short L31 cleaved by protease 7 reduces both activities Masami Ueta, Chieko Wada, Yoshitaka Bessho, Maki Maeda and Akira Wada. Genes Cells. 2017 May;22(5):452-471. https://doi.org/10.1111/gtc.12488
2017 Citation Dissecting limiting factors of the Protein synthesis Using Recombinant Elements (PURE) system Jun Li, Chi Zhang, Poyi Huang, Erkin Kuru, Eliot T C Forster-Benson, Taibo Li and George M Church. Translation (Austin). 2017 May 9;5(1):e1327006. ...
2017 Citation Cell-Free Translation Is More Variable than Transcription Fabio Chizzolini, Michele Forlin, Noël Yeh Martín, Giuliano Berloffa, Dario Cecchi and SheCitation S Mansy. ACS Synth Biol. 2017 Apr 21;6(4):638-647. h...
2017 Citation Cotranslational folding of spectrin domains via partially structured states Ola B Nilsson, Adrian A Nickson, Jeffrey J Hollins, Stephan Wickles, Annette Steward, Roland Beckmann, Gunnar von Heijne and Jane Clarke. Nat Struc...
2017 Citation Identification of a VapBC toxin-antitoxin system in a thermophilic bacterium Thermus thermophilus HB27 Yuqi Fan, Takayuki Hoshino and Akira Nakamura. Extremophiles. 2017 Jan;21(1):153-161. https://doi.org/10.1007/s00792-016-0891-1
2016 Citation Cell-Free Phospholipid Biosynthesis by Gene-Encoded Enzymes Reconstituted in Liposomes Andrew Scott, Marek J Noga, Paul de Graaf, Ilja Westerlaken, Esengul Yildirim and Christophe Danelon. PLoS One. 2016 Oct 6;11(10):e0163058. https:...
2016 Citation In vitro selection of bispecific diabody fragments using covalent bicistronic DNA display Masanao Nakayama, Shoko Komiya, Kei Fujiwara, Kenichi Horisawa and Nobuhide Doi. Biochem Biophys Res Commun. 2016 Sep 16;478(2):606-11. https://do...
2016 Citation Feedback control of Campylobacter jejuni flagellin levels through reciprocal binding of FliW to flagellin and the global regulator CsrA Katarzyna A Radomska, Soledad R Ordoñez, Marc M S M Wösten, Jaap A Wagenaar and Jos P M van Putten. Mol Microbiol. 2016 Oct;102(2):207-220. https:...
2016 Citation GreA and GreB Enhance Expression of Escherichia coli RNA Polymerase Promoters in a Reconstituted Transcription-Translation System Lea L de Maddalena, Henrike Niederholtmeyer, Matti Turtola, Zoe N Swank, Georgiy A Belogurov and Sebastian J Maerkl. ACS Synth Biol. 2016 Sep 16;5(...
2016 Citation Trigger Factor Reduces the Force Exerted on the Nascent Chain by a Cotranslationally Folding Protein Ola B Nilsson, Annika Müller-Lucks, Günter Kramer, Bernd Bukau and Gunnar von Heijne. J Mol Biol. 2016 Mar 27;428(6):1356-1364. https://doi.org/10...
2016 Citation Liposome chaperon in cell-free membrane protein synthesis: one-step preparation of KcsA-integrated liposomes and electrophysiological analysis by the planar bilayer method M Ando, M Akiyama, D Okuno, M Hirano, T Ide, S Sawada, Y Sasaki and K Akiyoshi. Biomater Sci. 2016 Feb;4(2):258-64. https://doi.org/10.1039/c5bm00...
2016 Citation Integrated in vivo and in vitro nascent chain profiling reveals widespread translational pausing Yuhei Chadani, Tatsuya Niwa, Shinobu Chiba, Hideki Taguchi and Koreaki Ito. Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):E829-38. https://doi.org/...
2015 Citation PURE mRNA display for in vitro selection of single-chain antibodies Yu Nagumo, Kei Fujiwara, Kenichi Horisawa, Hiroshi Yanagawa and Nobuhide Doi. J Biochem. 2016 May;159(5):519-26. https://doi.org/10.1093/jb/mvv131
2015 Citation Development of human neutralizing antibody to ADAMTS4 (aggrecanase-1) and ADAMTS5 (aggrecanase-2) Aya Shiraishi, Satsuki Mochizuki, Akira Miyakoshi, Kanehisa Kojoh and Yasunori Okada. Biochem Biophys Res Commun. 2016 Jan 1;469(1):62-69. https:/...
2015 Citation The Thermotolerant Yeast Kluyveromyces marxianus Is a Useful Organism for Structural and Biochemical Studies of Autophagy Hayashi Yamamoto, Takayuki Shima, Masaya Yamaguchi, Yuh Mochizuki, Hisashi Hoshida, Soichiro Kakuta, Chika Kondo-Kakuta, Nobuo N Noda, Fuyuhiko Ina...
2015 Citation Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria Eiji Ishii, Shinobu Chiba, Narimasa Hashimoto, Seiji Kojima, Michio Homma, Koreaki Ito, Yoshinori Akiyama and Hiroyuki Mori. Proc Natl Acad Sci U S...
2015 Citation Cotranslational Protein Folding inside the Ribosome Exit Tunnel Ola B Nilsson, Rickard Hedman, Jacopo Marino, Stephan Wickles, Lukas Bischoff, Magnus Johansson, Annika Müller-Lucks, Fabio Trovato, Joseph D Pugli...
2015 Citation Droplet-Shooting and Size-Filtration (DSSF) Method for Synthesis of Cell-Sized Liposomes with Controlled Lipid Compositions Muhoon Lee, Noriko Matsunaga, Shiori Akabane, Ippei Yasuda, Takuya Ueda and Nono Takeuchi-Tomita. Masamune Morita, Hiroaki Onoe, Miho Yanagisawa, H...
2015 Citation The PURE system for the cell-free synthesis of membrane proteins Yutetsu Kuruma and Takuya Ueda. Nat Protoc. 2015 Sep;10(9):1328-44. https://doi.org/10.1038/nprot.2015.082
2015 Citation Activities of 20 aminoacyl-tRNA synthetases expressed in a reconstituted translation system in Escherichia coli Takako Awai, Norikazu Ichihashi and Tetsuya Yomo. Biochem Biophys Rep. 2015 Aug 8;3:140-143. https://doi.org/10.1016/j.bbrep.2015.08.006
2015 Citation In vitro directed evolution of alpha-hemolysin by liposome display Satoshi Fujii, Tomoaki Matsuura and Tetsuya Yomo. Biophysics (Nagoya-shi). 2015 Mar 4;11:67-72. https://doi.org/10.2142/biophysics.11.67
2015 Citation Monitoring mRNA and protein levels in bulk and in model vesicle-based artificial cells Pauline van Nies, Alicia Soler Canton, Zohreh Nourian and Christophe Danelon. Methods Enzymol. 2015;550:187-214. https://doi.org/10.1016/bs.mie.20...
2015 Citation Simple identification of two causes of noise in an aptazyme system by monitoring cell-free transcription Norikazu Ichihashi, Shungo Kobori and Tetsuya Yomo. Methods Enzymol. 2015;550:93-107. https://doi.org/10.1016/bs.mie.2014.10.043
2014 Citation The PURE system for protein production Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori and Takuya Ueda. Methods Mol Biol. 2014;1118:275-84. https://doi.org/10.1007/978-1-62703-782-2...
2014 Citation Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device Hitoyoshi Yamashita, Masamune Morita, Haruka Sugiura, Kei Fujiwara, Hiroaki Onoe and Masahiro Takinoue. J Biosci Bioeng. 2015 Apr;119(4):492-5. ht...
2014 Citation Protein synthesis yield increased 72 times in the cell-free PURE system Kirsten Jackson, Takashi Kanamori, Takuya Ueda and Z Hugh Fan. Integr Biol (Camb). 2014 Aug;6(8):781-8. https://doi.org/10.1039/c4ib00088a
2014 Citation In vitro synthesis of the E. coli Sec translocon from DNA Hideaki Matsubayashi, Yutetsu Kuruma and Takuya Ueda. Angew Chem Int Ed Engl. 2014 Jul 14;53(29):7535-8. https://doi.org/10.1002/anie.201403929
2014 Citation Synthesis of milligram quantities of proteins using a reconstituted in vitro protein synthesis system Yasuaki Kazuta, Tomoaki Matsuura, Norikazu Ichihashi and Tetsuya Yomo. J Biosci Bioeng. 2014 Nov;118(5):554-7. https://doi.org/10.1016/j.jbiosc.20...
2014 Citation Toward the assembly of a minimal divisome Zohreh Nourian, Andrew Scott and Christophe Danelon. Syst Synth Biol. 2014 Sep;8(3):237-47. https://doi.org/10.1007/s11693-014-9150-x
2014 Citation PURE ribosome display and its application in antibody technology Takashi Kanamori, Yasuhiro Fujino and Takuya Ueda. Biochim Biophys Acta. 2014 Nov;1844(11):1925-1932. https://doi.org/10.1016/j.bbapap.2014.04.007
2014 Citation Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation Seok Hoon Hong, Ioanna Ntai, Adrian D Haimovich, Neil L Kelleher, Farren J Isaacs and Michael C Jewett. ACS Synth Biol. 2014 Jun 20;3(6):398-409. ...
2014 Citation Noncanonical structures and their thermodynamics of DNA and RNA under molecular crowding: beyond the Watson-Crick double helix Naoki Sugimoto. Int Rev Cell Mol Biol. 2014;307:205-73. https://doi.org/10.1016/b978-0-12-800046-5.00008-4
2013 Citation Gene position more strongly influences cell-free protein expression from operons than T7 transcriptional promoter strength Fabio Chizzolini, Michele Forlin, Dario Cecchi and SheCitation S Mansy. ACS Synth Biol. 2014 Jun 20;3(6):363-71. https://doi.org/10.1021/sb4000977
2013 Citation Identification of residues required for stalled-ribosome rescue in the codon-independent release factor YaeJ Hiroyuki Kogure, Yoshihiro Handa, Masahiro Nagata, Naoto Kanai, Peter Güntert, Kenji Kubota and Nobukazu Nameki. Nucleic Acids Res. 2014 Mar;42(5):...
2013 Citation In vitro evolution of α-hemolysin using a liposome display Satoshi Fujii, Tomoaki Matsuura, Takeshi Sunami, Yasuaki Kazuta and Tetsuya Yomo. Proc Natl Acad Sci USA. 2013 Oct 15;110(42):16796-801. https://d...
2013 Citation Implementation of cell-free biological networks at steady state Henrike Niederholtmeyer, Viktoria Stepanova and Sebastian J Maerkl. Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):15985-90. https://doi.org/10.1073...
2013 Citation Unbiased tracking of the progression of mRNA and protein synthesis in bulk and in liposome-confined reactions Pauline van Nies, Zohreh Nourian, Maurits Kok, Roeland van Wijk, Jonne Moeskops, Ilja Westerlaken, Jos M Poolman, Rienk Eelkema, Jan H van Esch, Yu...
2013 Citation In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation Michael C Jewett, Brian R Fritz, Laura E Timmerman and George M Church. Mol Syst Biol. 2013 Jun 25;9:678. https://doi.org/10.1038/msb.2013.31
2013 Citation Translational halt during elongation caused by G-quadruplex formed by mRNA Tamaki Endoh, Yu Kawasaki and Naoki Sugimoto. Methods. 2013 Nov;64(1):73-8. https://doi.org/10.1016/j.ymeth.2013.05.026
2013 Citation Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system Kei Fujiwara, Tsutomu Katayama and Shin-Ichiro M Nomura. Nucleic Acids Res. 2013 Aug;41(14):7176-83. https://doi.org/10.1093/nar/gkt489
2013 Citation Fluorescent proteins and in vitro genetic organization for cell-free synthetic biology Roberta Lentini, Michele Forlin, Laura Martini, Cristina Del Bianco, Amy C Spencer, Domenica Torino and SheCitation S Mansy. ACS Synth Biol. 2013 Sep 20...
2013 Citation Stability of RNA quadruplex in open reading frame determines proteolysis of human estrogen receptor α Tamaki Endoh, Yu Kawasaki and Naoki Sugimoto. Nucleic Acids Res. 2013 Jul;41(12):6222-31. https://doi.org/10.1093/nar/gkt286
2013 Citation Suppression of gene expression by G-quadruplexes in open reading frames depends on G-quadruplex stability Tamaki Endoh, Yu Kawasaki and Naoki Sugimoto. Angew Chem Int Ed Engl. 2013 May 17;52(21):5522-6. https://doi.org/10.1002/anie.201300058
2013 Citation Nascent peptides that block protein synthesis in bacteria Christopher J Woolstenhulme, Shankar Parajuli, David W Healey, Diana P Valverde, E Nicholas Petersen, Agata L Starosta, Nicholas R Guydosh, W Evan ...
2013 Citation Comparative evaluation of two cell-free protein synthesis systems derived from Escherichia coli for genetic code reprogramming Ki Baek Lee, Ho-Cheol Kim, Dong-Myung Kim, Taek Jin Kang and Hiroaki Suga. J Biotechnol. 2012 Dec 15;164(2):330-5. https://doi.org/10.1016/j.jbiot...
2012 Citation Real-time mRNA measurement during an in vitro transcription and translation reaction using binary probes Henrike Niederholtmeyer, Ling Xu and Sebastian J Maerkl. ACS Synth Biol. 2013 Aug 16;2(8):411-7. https://doi.org/10.1021/sb300104f
2012 Citation Robust in vitro affinity maturation strategy based on interface-focused high-throughput mutational scanning Yasuhiro Fujino, Risako Fujita, Kouichi Wada, Kotomi Fujishige, Takashi Kanamori, Lindsey Hunt, Yoshihiro Shimizu and Takuya Ueda. Biochem Biophys ...
2012 Citation Affinity maturation and functional dissection of a humanised anti-RAGE monoclonal antibody by ribosome display Simon E Hufton. Methods Mol Biol. 2012;805:403-22. https://doi.org/10.1007/978-1-61779-379-0_23
2012 Citation In vitro selection of unnatural cyclic peptide libraries via mRNA display Zhong Ma and Matthew C T Hartman. Methods Mol Biol. 2012;805:367-90. https://doi.org/10.1007/978-1-61779-379-0_21
2012 Citation Peptide screening using PURE ribosome display Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada, Bintang K Akbar and Takuya Ueda. Methods Mol Biol. 2012;805:251-9. https://doi.org/10.1007/978-1-6...
2012 Citation SIRT5 deacetylates and activates urate oxidase in liver mitochondria of mice Yasuhiko Nakamura, Masahito Ogura, Kasane Ogura, Daisuke Tanaka and Nobuya Inagaki. FEBS Lett. 2012 Nov 30;586(23):4076-81. https://doi.org/10.101...
2012 Citation In vitro selection of fab fragments by mRNA display and gene-linking emulsion PCR Takeshi Sumida, Hiroshi Yanagawa and Nobuhide Doi. J Nucleic Acids. 2012;2012:371379. https://doi.org/10.1155/2012/371379
2012 Citation ArfA recruits RF2 into stalled ribosomes Yoshihiro Shimizu. J Mol Biol. 2012 Nov 2;423(4):624-31. https://doi.org/10.1016/j.jmb.2012.08.007
2012 Citation Directed Evolution of Proteins through In Vitro Protein Synthesis in Liposomes Takehiro Nishikawa, Takeshi Sunami, Tomoaki Matsuura and Tetsuya Yomo. J Nucleic Acids. 2012;2012:923214. https://doi.org/10.1155/2012/923214
2012 Citation Nanoproteomics enabling personalized nanomedicine Claudio Nicolini, Nicola Bragazzi and Eugenia Pechkova. Adv Drug Deliv Rev. 2012 Oct;64(13):1522-31. https://doi.org/10.1016/j.addr.2012.06.015
2012 Citation A Concept for Selection of Codon-Suppressor tRNAs Based on Read-Through Ribosome Display in an In Vitro Compartmentalized Cell-Free Translation System Atsushi Ogawa, Masayoshi Hayami, Shinsuke Sando and Yasuhiro Aoyama. J Nucleic Acids. 2012;2012:538129. https://doi.org/10.1155/2012/538129
2012 Citation Precursor directed biosynthesis of an orthogonally functional erythromycin analogue: selectivity in the ribosome macrolide binding pocket Colin J B Harvey, Joseph D Puglisi, Vijay S Pande, David E Cane and Chaitan Khosla. J Am Chem Soc. 2012 Jul 25;134(29):12259-65. https://doi.org/1...
2012 Citation Effects of compartment size on the kinetics of intracompartmental multimeric protein synthesis Tomoaki Matsuura, Kazufumi Hosoda, Yasuaki Kazuta, Norikazu Ichihashi, Hiroaki Suzuki and Tetsuya Yomo. ACS Synth Biol. 2012 Sep 21;1(9):431-7. ht...
2012 Citation Elongation factor G is a critical target during oxidative damage to the translation system of Escherichia coli Takanori Nagano, Kouji Kojima, Toru Hisabori, Hidenori Hayashi, Eugene Hayato Morita, Takashi Kanamori, Tomoko Miyagi, Takuya Ueda and Yoshitaka Ni...
2012 Citation Kinetic analysis of aptazyme-regulated gene expression in a cell-free translation system: modeling of ligand-dependent and -independent expression Shungo Kobori, Norikazu Ichihashi, Yasuaki Kazuta, Tomoaki Matsuura and Tetsuya Yomo. RNA. 2012 Aug;18(8):1458-65. https://doi.org/10.1261/rna.032...
2012 Citation In vitro selection of highly modified cyclic peptides that act as tight binding inhibitors Yollete V Guillen Schlippe, Matthew C T Hartman, Kristopher Josephson and Jack W Szostak. J Am Chem Soc. 2012 Jun 27;134(25):10469-77. https://doi...
2012 Citation Cell-free protein synthesis from a single copy of DNA in a glass microchamber Taiji Okano, Tomoaki Matsuura, Yasuaki Kazuta, Hiroaki Suzuki and Tetsuya Yomo. Lab Chip. 2012 Aug 7;12(15):2704-11. https://doi.org/10.1039/c2lc4...
2012 Citation Broad-specificity mRNA-rRNA complementarity in efficient protein translation Pamela A Barendt, Najaf A Shah, Gregory A Barendt and Casim A Sarkar. PLoS Genet. 2012;8(3):e1002598. https://doi.org/10.1371/journal.pgen.1002598
2012 Citation Cell-free protein synthesis inside giant unilamellar vesicles analyzed by flow cytometry Koji Nishimura, Tomoaki Matsuura, Kazuya Nishimura, Takeshi Sunami, Hiroaki Suzuki and Tetsuya Yomo. Langmuir. 2012 Jun 5;28(22):8426-32. https://...
2012 Citation Global analysis of chaperone effects using a reconstituted cell-free translation system Tatsuya Niwa, Takashi Kanamori, Takuya Ueda and Hideki Taguchi. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8937-42. https://doi.org/10.1073/pnas...
2012 Citation Real-time assay for testing components of protein synthesis Gabriel Rosenblum, Chunlai Chen, Jaskiran Kaur, Xiaonan Cui, Yale E Goldman and Barry S Cooperman. Nucleic Acids Res. 2012 Jul;40(12):e88. https:/...
2012 Citation Characterization of the emergent properties of a synthetic quasi-cellular system Lorenzo Lazzerini-Ospri, Pasquale Stano, PierLuigi Luisi and Roberto Marangoni. BMC Bioinformatics. 2012 Mar 28;13 Suppl 4(Suppl 4):S9. https://do...
2012 Citation The ancestral SgrS RNA discriminates horizontally acquired Salmonella mRNAs through a single G-U wobble pair Kai Papenfort, Dimitri Podkaminski, Jay C D Hinton and Jörg Vogel. Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):E757-64. https://doi.org/10.1073/...
2012 Citation Target specificity of an autoreactive pathogenic human γδ-T cell receptor in myositis Jessica Bruder, Katherina Siewert, Birgit Obermeier, Joachim Malotka, Peter Scheinert, Josef Kellermann, Takuya Ueda, Reinhard Hohlfeld and Klaus D...
2012 Citation Mutant p53 cooperates with ETS2 to promote etoposide resistance Phi M Do, Lakshman Varanasi, Songqing Fan, Chunyang Li, Iwona Kubacka, Virginia Newman, Krishna Chauhan, Silvano Rakeem Daniels, Maurizio Boccetta,...
2012 Citation Traveling Time of a translating ribosome along messenger RNA monitored directly on a quartz crystal microbalance Shuntaro Takahashi, Kentaro Tsuji, Takuya Ueda and Yoshio Okahata. J Am Chem Soc. 2012 Apr 18;134(15):6793-800. https://doi.org/10.1021/ja300993d
2012 Citation Analysis of p53 binding to DNA by fluorescence imaging microscopy Hwee Jing Ong, Jia Wei Siau, Jing Bo Zhang, Minghui Hong, Horst Flotow and Farid Ghadessy. Micron. 2012 Sep;43(9):996-1000. https://doi.org/10.101...
2012 Citation Experiment and mathematical modeling of gene expression dynamics in a cell-free system Tobias Stögbauer, Lukas Windhager, Ralf Zimmer and Joachim O Rädler. Integr Biol (Camb). 2012 May;4(5):494-501. https://doi.org/10.1039/c2ib00102k
2012 Citation Modification-free photocontrol of β-lactam conversion with spatiotemporal resolution Anna Venancio-Marques, Yan-Jun Liu, Antoine Diguet, Thomas di Maio, Arnaud Gautier and Damien Baigl. ACS Synth Biol. 2012 Nov 16;1(11):526-31. htt...
2012 Citation Differential regulation of mesodermal gene expression by Drosophila cell type-specific Forkhead transcription factors Xianmin Zhu, Shaad M Ahmad, Anton Aboukhalil, Brian W Busser, Yongsok Kim, Terese R Tansey, Adrian Haimovich, Neal Jeffries, Martha L Bulyk and Ala...
2012 Citation Multiplexed in vivo His-tagging of enzyme pathways for in vitro single-pot multienzyme catalysis Harris H Wang, Po-Yi Huang, George Xu, Wilhelm Haas, Adam Marblestone, Jun Li, Steven P Gygi, Anthony C Forster, Michael C Jewett and George M Chur...
2012 Citation A mixed double negative feedback loop between the sRNA MicF and the global regulator Lrp Erik Holmqvist, Cecilia Unoson, Johan Reimegård and E Gerhart H Wagner. Mol Microbiol. 2012 May;84(3):414-27. https://doi.org/10.1111/j.1365-2958....
2012 Citation Triggered gene expression in fed-vesicle microreactors with a multifunctional membrane Zohreh Nourian, Wouter Roelofsen and Christophe Danelon. Angew Chem Int Ed Engl. 2012 Mar 26;51(13):3114-8. https://doi.org/10.1002/anie.201107123
2012 Citation DNA display selection of peptide ligands for a full-length human G protein-coupled receptor on CHO-K1 cells Nobuhide Doi, Natsuko Yamakawa, Hideaki Matsumoto, Yasutsugu Yamamoto, Tetsuya Nagano, Nobutaka Matsumura, Kenichi Horisawa and Hiroshi Yanagawa. P...
2012 Citation Alternative splicing of the auxin biosynthesis gene YUCCA4 determines its subcellular compartmentation Verena Kriechbaumer, Pengwei Wang, Chris Hawes and Ben M Abell. Plant J. 2012 Apr;70(2):292-302. https://doi.org/10.1111/j.1365-313x.2011.04866.x
2011 Citation The ribosome modulates nascent protein folding Christian M Kaiser, Daniel H Goldman, John D Chodera, Ignacio Tinoco Jr and Carlos Bustamante. Science. 2011 Dec 23;334(6063):1723-7. https://doi....
2011 Citation Synchronized translation for detection of temporal stalling of ribosome during single-turnover translation Tamaki Endoh, Yu Kawasaki and Naoki Sugimoto. Anal Chem. 2012 Jan 17;84(2):857-61. https://doi.org/10.1021/ac202712g
2011 Citation Knot formation in newly translated proteins is spontaneous and accelerated by chaperonins Anna L Mallam and Sophie E Jackson. Nat Chem Biol. 2011 Dec 18;8(2):147-53. https://doi.org/10.1038/nchembio.742
2011 Citation Expression optimization and synthetic gene networks in cell-free systems David K Karig, Sukanya Iyer, Michael L Simpson and Mitchel J Doktycz. Nucleic Acids Res. 2012 Apr;40(8):3763-74. https://doi.org/10.1093/nar/gkr1191
2011 Citation Clostridium difficile cell wall protein CwpV undergoes enzyme-independent intramolecular autoproteolysis Marcin Dembek, Catherine B Reynolds and Neil F Fairweather. J Biol Chem. 2012 Jan 6;287(2):1538-44. https://doi.org/10.1074/jbc.m111.302463
2011 Citation Reconstitution of the human chaperonin CCT by co-expression of the eight distinct subunits in mammalian cells Kodai Machida, Mamiko Masutani, Tominari Kobayashi, Satoshi Mikami, Yuri Nishino, Atsuo Miyazawa and Hiroaki Imataka. Protein Expr Purif. 2012 Mar;...
2011 Citation A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins Cassandra Guarino and Matthew P DeLisa. Glycobiology. 2012 May;22(5):596-601. https://doi.org/10.1093/glycob/cwr151
2011 Citation Characterization of type II and III restriction-modification systems from Bacillus cereus strains ATCC 10987 and ATCC 14579 Shuang-yong Xu, Rebecca L Nugent, Julie Kasamkattil, Alexey Fomenkov, Yogesh Gupta, Aneel Aggarwal, Xiaolong Wang, Zhiru Li, Yu Zheng and Richard M...
2011 Citation Zn(II) binding to Escherichia coli 70S ribosomes M Patrick Hensley, David L Tierney and Michael W Crowder. Biochemistry. 2011 Nov 22;50(46):9937-9. https://doi.org/10.1021/bi200619w
2011 Citation Synthesis of [11C]interleukin 8 using a cell-free translation system and L-[11C]methionine Ryuichi Harada, Shozo Furumoto, Takeo Yoshikawa, Yoichi Ishikawa, Katsuhiko Shibuya, Nobuyuki Okamura, Ren Iwata and Kazuhiko Yanai. Nucl Med Biol....
2011 Citation Spontaneous crowding of ribosomes and proteins inside vesicles: a possible mechanism for the origin of cell metabolism Tereza Pereira de Souza, Frank Steiniger, Pasquale Stano, Alfred Fahr and Pier Luigi Luisi. Chembiochem. 2011 Oct 17;12(15):2325-30. https://doi....
2011 Citation Polypeptide aptamer selection using a stabilized ribosome display Wei Wang, Shuta Hara, Mingzhe Liu, Toshiro Aigaki, Shigeru Shimizu and Yoshihiro Ito. J Biosci Bioeng. 2011 Nov;112(5):515-7. https://doi.org/10.1...
2011 Citation Luminescence resonance energy transfer in the cytoplasm of live Escherichia coli cells Daniel González, Nayanish Lokhande, Swaraj Vadde, Qi Zhao, Aaron Cassill and Robert Renthal. Biochemistry. 2011 Aug 16;50(32):6789-96. https://doi...
2011 Citation Heat shock factor binds to heat shock elements upstream of heat shock protein 70a and Samui genes to confer transcriptional activity in Bombyx mori diapause eggs exposed to 5°C Fukashi Kihara, Teruyuki Niimi, Okitsugu Yamashita and Toshinobu Yaginuma. Insect Biochem Mol Biol. 2011 Nov;41(11):843-51. https://doi.org/10.101...
2011 Citation Synthesis and in situ insertion of a site-specific fluorescently labeled membrane protein into cell-sized liposomes Takuma Ohtsuka, Satoshi Neki, Tamotsu Kanai, Kazunari Akiyoshi, Shin-ichiro M Nomura and Takashi Ohtsuki. Anal Biochem. 2011 Nov 1;418(1):97-101. ...
2011 Citation In vitro selection of proteins that undergo covalent labeling with small molecules by thiol-disulfide exchange by using ribosome display Hayato Yanagida, Tomoaki Matsuura, Yasuaki Kazuta and Tetsuya Yomo. Chembiochem. 2011 Apr 11;12(6):962-9. https://doi.org/10.1002/cbic.201000620
2011 Citation Kinetic study of de novo chromophore maturation of fluorescent proteins Ryo Iizuka, Mai Yamagishi-Shirasaki and Takashi Funatsu. Anal Biochem. 2011 Jul 15;414(2):173-8. https://doi.org/10.1016/j.ab.2011.03.036
2011 Citation tmRNA regulates synthesis of the ArfA ribosome rescue factor Fernando Garza-Sánchez, Ryan E Schaub, Brian D Janssen and Christopher S Hayes. Mol Microbiol. 2011 Jun;80(5):1204-19. https://doi.org/10.1111/j.1...
2011 Citation Localization of BiP to translating ribosomes increases soluble accumulation of secreted eukaryotic proteins in an Escherichia coli cell-free system John P Welsh, Jeanne Bonomo and James R Swartz. Biotechnol Bioeng. 2011 Aug;108(8):1739-48. https://doi.org/10.1002/bit.23111
2011 Citation Recruitment of a species-specific translational arrest module to monitor different cellular processes Shinobu Chiba, Takashi Kanamori, Takuya Ueda, Yoshinori Akiyama, Kit Pogliano and Koreaki Ito. Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):6073-8...
2011 Citation Optimal codon choice can improve the efficiency and fidelity of N-methyl amino acid incorporation into peptides by in-vitro translation Alexander O Subtelny, Matthew C T Hartman and Jack W Szostak. Angew Chem Int Ed Engl. 2011 Mar 28;50(14):3164-7. https://doi.org/10.1002/anie.2010...
2011 Citation Using a structural and logics systems approach to infer bHLH-DNA binding specificity determinants Federico De Masi, Christian A Grove, Anastasia Vedenko, Andreu Alibés, Stephen S Gisselbrecht, Luis Serrano, Martha L Bulyk and Albertha J M Walhou...
2011 Citation Sequence specificity is obtained from the majority of modular C2H2 zinc-finger arrays Kathy N Lam, Harm van Bakel, Atina G Cote, Anton van der Ven and Timothy R Hughes. Nucleic Acids Res. 2011 Jun;39(11):4680-90. https://doi.org/10....
2011 Citation Identification of a chitin-induced small RNA that regulates translation of the tfoX gene, encoding a positive regulator of natural competence in Vibrio cholerae Shouji Yamamoto, Hidemasa Izumiya, Jiro Mitobe, Masatomo Morita, Eiji Arakawa, Makoto Ohnishi and Haruo Watanabe. J Bacteriol. 2011 Apr;193(8):1953...
2011 Citation Nascent peptide in the ribosome exit tunnel affects functional properties of the A-site of the peptidyl transferase center Haripriya Ramu, Nora Vázquez-Laslop, Dorota Klepacki, Qing Dai, Joseph Piccirilli, Ronald Micura and Alexander S Mankin. Mol Cell. 2011 Feb 4;41(3)...
2011 Citation Improvement of translation efficiency in an Escherichia coli cell-free protein system using cysteine Tomoteru Shingaki and Noriyuki Nimura. Protein Expr Purif. 2011 Jun;77(2):193-7. https://doi.org/10.1016/j.pep.2011.01.017
2011 Citation Selection of peptides targeting helix 31 of bacterial 16S ribosomal RNA by screening M13 phage-display libraries Tek N Lamichhane, N Dinuka Abeydeera, Anne-Cécile E Duc, Philip R Cunningham and Christine S Chow. Molecules. 2011 Jan 28;16(2):1211-39. https://d...
2011 Citation Single molecule imaging of the trans-translation entry process via anchoring of the tagged ribosome Zhan-Ping Zhou, Yoshihiro Shimizu, Hisashi Tadakuma, Hideki Taguchi, Koichi Ito and Takuya Ueda. J Biochem. 2011 May;149(5):609-18. https://doi.or...
2011 Citation The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes Christakis Panayiotou, Nicola Solaroli, Yunjian Xu, Magnus Johansson and Anna Karlsson. Biochem J. 2011 Feb 1;433(3):527-34. https://doi.org/10.10...
2011 Citation Engineering a waste management enzyme to overcome cancer resistance to apoptosis: adding DNase1 to the anti-cancer toolbox K Rosner, M F Kasprzak, A C J Horenstein, H L Thurston, J Abrams, L Y Kerwin, D A Mehregan and D R Mehregan. Cancer Gene Ther. 2011 May;18(5):346-5...
2011 Citation Ribosome display selection of a murine IgG₁ Fab binding affibody molecule allowing species selective recovery of monoclonal antibodies Sebastian Grimm, Feifan Yu and Per-Åke Nygren. Mol Biotechnol. 2011 Jul;48(3):263-76. https://doi.org/10.1007/s12033-010-9367-1
2010 Citation YaeJ is a novel ribosome-associated protein in Escherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes Yoshihiro Handa, Noriyuki Inaho and Nobukazu Nameki. Nucleic Acids Res. 2011 Mar;39(5):1739-48. https://doi.org/10.1093/nar/gkq1097
2010 Citation Docking-dependent ubiquitination of the interferon regulatory factor-1 tumor suppressor protein by the ubiquitin ligase CHIP Vikram Narayan, Emmanuelle Pion, Vivien Landré, Petr Müller and Kathryn L Ball. J Biol Chem. 2011 Jan 7;286(1):607-19. https://doi.org/10.1074/jbc...
2010 Citation Mutational and biochemical analysis of the DNA-entry nuclease EndA from Streptococcus pneumoniae Marika Midon, Patrick Schäfer, Alfred Pingoud, Mahua Ghosh, Andrea F Moon, Matthew J Cuneo, Robert E London and Gregor Meiss. Nucleic Acids Res. 20...
2010 Citation Direct integration of cell-free-synthesized connexin-43 into liposomes and hemichannel formation Yuki Moritani, Shin-ichiro M Nomura, Ikuo Morita and Kazunari Akiyoshi. FEBS J. 2010 Aug;277(16):3343-52. https://doi.org/10.1111/j.1742-4658.2010...
2010 Citation Versatility of trigger factor interactions with ribosome-nascent chain complexes Sathish Kumar Lakshmipathy, Rashmi Gupta, Stefan Pinkert, Stephanie Anne Etchells and F Ulrich Hartl. J Biol Chem. 2010 Sep 3;285(36):27911-23. ht...
2010 Citation The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex Gandhi Theerthagiri, Nathalie Eisenhardt, Heinz Schwarz and Wolfram Antonin. J Cell Biol. 2010 Jun 28;189(7):1129-42. https://doi.org/10.1083/jcb....
2010 Citation Cortisol is involved in temperature-dependent sex determination in the Japanese flounder Toshiya Yamaguchi, Norifumi Yoshinaga, Takashi Yazawa, Koichiro Gen and Takeshi Kitano. Endocrinology. 2010 Aug;151(8):3900-8. https://doi.org/10....
2010 Citation Unusual target site disruption by the rare-cutting HNH restriction endonuclease PacI Betty W Shen, Daniel F Heiter, Siu-Hong Chan, Hua Wang, Shuang-Yong Xu, Richard D Morgan, Geoffrey G Wilson and Barry L Stoddard. Structure. 2010 J...
2010 Citation Cellular compartment model for exploring the effect of the lipidic membrane on the kinetics of encapsulated biochemical reactions Takeshi Sunami, Kazufumi Hosoda, Hiroaki Suzuki, Tomoaki Matsuura and Tetsuya Yomo. Langmuir. 2010 Jun 1;26(11):8544-51. https://doi.org/10.1021/l...
2010 Citation In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme Haruichi Asahara and Shaorong Chong. Nucleic Acids Res. 2010 Jul;38(13):e141. https://doi.org/10.1093/nar/gkq377
2010 Citation Two antisense RNAs target the transcriptional regulator CsgD to inhibit curli synthesis Erik Holmqvist, Johan Reimegård, Maaike Sterk, Nina Grantcharova, Ute Römling and Eduard Gerhart Heinrich Wagner. EMBO J. 2010 Jun 2;29(11):1840-50...
2010 Citation Comparing the functional properties of the Hsp70 chaperones, DnaK and BiP Jeanne Bonomo, John P Welsh, Karthish Manthiram and James R Swartz. Biophys Chem. 2010 Jun;149(1-2):58-66. https://doi.org/10.1016/j.bpc.2010.04.001
2010 Citation A novel complete reconstitution system for membrane integration of the simplest membrane protein Ken-ichi Nishiyama, Masahide Maeda, Masato Abe, Takashi Kanamori, Keiko Shimamoto, Shoichi Kusumoto, Takuya Ueda and Hajime Tokuda. Biochem Biophys...
2010 Citation The Tetrahymena argonaute-binding protein Giw1p directs a mature argonaute-siRNA complex to the nucleus Tomoko Noto, Henriette M Kurth, Kensuke Kataoka, Lucia Aronica, Leroi V DeSouza, K W Michael Siu, Ronald E Pearlman, Martin A Gorovsky and Kazufumi...
2010 Citation mRNA display selection of a high-affinity, Bcl-X(L)-specific binding peptide Nobutaka Matsumura, Toru Tsuji, Takeshi Sumida, Masahito Kokubo, Michiko Onimaru, Nobuhide Doi, Hideaki Takashima, Etsuko Miyamoto-Sato and Hiroshi...
2009 Citation Bicistronic DNA display for in vitro selection of Fab fragments Takeshi Sumida, Nobuhide Doi and Hiroshi Yanagawa. Nucleic Acids Res. 2009 Dec;37(22):e147. https://doi.org/10.1093/nar/gkp776
2009 Citation PURE technology Yoshihiro Shimizu and Takuya Ueda. Methods Mol Biol. 2010;607:11-21. https://doi.org/10.1007/978-1-60327-331-2_2
2009 Citation Production of multi-subunit complexes on liposome through an E. coli cell-free expression system Yutetsu Kuruma, Toshiharu Suzuki and Takuya Ueda. Methods Mol Biol. 2010;607:161-71. https://doi.org/10.1007/978-1-60327-331-2_14
2009 Citation Ribosome display with the PURE technology Takuya Ueda, Takashi Kanamori and Hiroyuki Ohashi. Methods Mol Biol. 2010;607:219-25. https://doi.org/10.1007/978-1-60327-331-2_18
2009 Citation Genetic identification of nascent peptides that induce ribosome stalling Douglas R Tanner, Daniel A Cariello, Christopher J Woolstenhulme, Mark A Broadbent and Allen R Buskirk. J Biol Chem. 2009 Dec 11;284(50):34809-18. ...
2009 Citation Harmine specifically inhibits protein kinase DYRK1A and interferes with neurite formation Nora Göckler, Guillermo Jofre, Chrisovalantis Papadopoulos, Ulf Soppa, Francisco J Tejedor and Walter Becker. FEBS J. 2009 Nov;276(21):6324-37. ht...
2009 Citation Salmonella enterica serotype Typhimurium DT104 ArtA-dependent modification of pertussis toxin-sensitive G proteins in the presence of [32P]NAD Ikuo Uchida, Ryoko Ishihara, Kiyoshi Tanaka, Eiji Hata, Sou-Ichi Makino, Toru Kanno, Shinichi Hatama, Masato Kishima, Masato Akiba, Atsushi Watanab...
2009 Citation Quantifying epistatic interactions among the components constituting the protein translation system Tomoaki Matsuura, Yasuaki Kazuta, Takuyo Aita, Jiro Adachi and Tetsuya Yomo. Mol Syst Biol. 2009;5:297. https://doi.org/10.1038/msb.2009.50
2009 Citation A new member of the Escherichia coli fad regulon: transcriptional regulation of fadM (ybaW) Youjun Feng and John E Cronan. J Bacteriol. 2009 Oct;191(20):6320-8. https://doi.org/10.1128/jb.00835-09
2009 Citation Identification of two active functional domains of human adenylate kinase 5 Nicola Solaroli, Christakis Panayiotou, Magnus Johansson and Anna Karlsson. FEBS Lett. 2009 Sep 3;583(17):2872-6. https://doi.org/10.1016/j.febsle...
2009 Citation Profiling of gene-dependent translational progress in cell-free protein synthesis by real-space imaging Eriko Mikamo-Satoh, Akihiko Takagi, Hiroyuki Tanaka, Takuya Matsumoto, Tsutomu Nishihara and Tomoji Kawai. Anal Biochem. 2009 Nov 15;394(2):275-80....
2009 Citation Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation Verena Pfeiffer, Kai Papenfort, Sacha Lucchini, Jay C D Hinton and Jörg Vogel. Nat Struct Mol Biol. 2009 Aug;16(8):840-6. https://doi.org/10.1038/...
2009 Citation Roles of Tom70 in import of presequence-containing mitochondrial proteins Hayashi Yamamoto, Kenji Fukui, Hisashi Takahashi, Shingo Kitamura, Takuya Shiota, Kayoko Terao, Mayumi Uchida, Masatoshi Esaki, Shuh-ichi Nishikawa...
2009 Citation Sequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder André Estévez-Torres, Cécile Crozatier, Antoine Diguet, Tomoaki Hara, Hirohide Saito, Kenichi Yoshikawa and Damien Baigl. Proc Natl Acad Sci U S A....
2009 Citation Real-time monitoring of cell-free translation on a quartz-crystal microbalance Shuntaro Takahashi, Masaaki Iida, Hiroyuki Furusawa, Yoshihiro Shimizu, Takuya Ueda and Yoshio Okahata. J Am Chem Soc. 2009 Jul 8;131(26):9326-32. ...
2009 Citation Interleukin-33 is biologically active independently of caspase-1 cleavage Dominique Talabot-Ayer, Céline Lamacchia, Cem Gabay and Gaby Palmer. J Biol Chem. 2009 Jul 17;284(29):19420-6. https://doi.org/10.1074/jbc.m901744...
2009 Citation Analysis of nonstop mRNA translation in the absence of tmRNA in Escherichia coli Kazushige Kuroha, Nobuo Horiguchi, Hiroji Aiba and Toshifumi Inada. Genes Cells. 2009 Jun;14(6):739-49. https://doi.org/10.1111/j.1365-2443.2009.0...
2009 Citation Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteins Tatsuya Niwa, Bei-Wen Ying, Katsuyo Saito, WenZhen Jin, Shoji Takada, Takuya Ueda and Hideki Taguchi. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11)...
2009 Citation Epitope mapping using ribosome display in a reconstituted cell-free protein synthesis system Eriko Osada, Yoshihiro Shimizu, Bintang K Akbar, Takashi Kanamori and Takuya Ueda. J Biochem. 2009 May;145(5):693-700. https://doi.org/10.1093/jb/...
2009 Citation Trigger factor from the psychrophilic bacterium Psychrobacter frigidicola is a monomeric chaperone Sylvain Robin, Denisio M Togashi, Alan G Ryder and J Gerard Wall. J Bacteriol. 2009 Feb;191(4):1162-8. https://doi.org/10.1128/jb.01137-08
2009 Citation A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells Yutetsu Kuruma, Pasquale Stano, Takuya Ueda and Pier Luigi Luisi. Biochim Biophys Acta. 2009 Feb;1788(2):567-74. https://doi.org/10.1016/j.bbamem....
2009 Citation Using shotgun sequence data to find active restriction enzyme genes Yu Zheng, Janos Posfai, Richard D Morgan, Tamas Vincze and Richard J Roberts. Nucleic Acids Res. 2009 Jan;37(1):e1. https://doi.org/10.1093/nar/gk...
2008 Citation Quantitative study of the structure of multilamellar giant liposomes as a container of protein synthesis reaction Kazufumi Hosoda, Takeshi Sunami, Yasuaki Kazuta, Tomoaki Matsuura, Hiroaki Suzuki and Tetsuya Yomo. Langmuir. 2008 Dec 2;24(23):13540-8. https://d...
2008 Citation Cell-free synthesis of the torque-generating membrane proteins, PomA and PomB, of the Na+-driven flagellar motor in Vibrio alginolyticus Hiroyuki Terashima, Rei Abe-Yoshizumi, Seiji Kojima and Michio Homma. J Biochem. 2008 Nov;144(5):635-42. https://doi.org/10.1093/jb/mvn110
2008 Citation A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based Jason R Hillebrecht and Shaorong Chong. BMC Biotechnol. 2008 Jul 29;8:58. https://doi.org/10.1186/1472-6750-8-58
2008 Citation RNA, but not protein partners, is directly responsible for translational silencing by a bacterial Hfq-binding small RNA Kimika Maki, Kanako Uno, Teppei Morita and Hiroji Aiba. Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10332-7. https://doi.org/10.1073/pnas.080310...
2008 Citation Initiating translation with D-amino acids Yuki Goto, Hiroshi Murakami and Hiroaki Suga. RNA. 2008 Jul;14(7):1390-8. https://doi.org/10.1261/rna.1020708
2008 Citation Single-molecule imaging of full protein synthesis by immobilized ribosomes Sotaro Uemura, Ryo Iizuka, Taro Ueno, Yoshihiro Shimizu, Hideki Taguchi, Takuya Ueda, Joseph D Puglisi and Takashi Funatsu. Nucleic Acids Res. 2008...
2008 Citation Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation Johannes H Urban and Jörg Vogel. PLoS Biol. 2008 Mar 18;6(3):e64. https://doi.org/10.1371/journal.pbio.0060064
2008 Citation The BsaHI restriction-modification system: cloning, sequencing and analysis of conserved motifs Robert K Neely and Richard J Roberts. BMC Mol Biol. 2008 May 14;9:48. https://doi.org/10.1186/1471-2199-9-48
2008 Citation Ribosomal synthesis of peptidase-resistant peptides closed by a nonreducible inter-side-chain bond Yusuke Sako, Yuki Goto, Hiroshi Murakami and Hiroaki Suga. ACS Chem Biol. 2008 Apr 18;3(4):241-9. https://doi.org/10.1021/cb800010p
2008 Citation Ribosomal synthesis of bicyclic peptides via two orthogonal inter-side-chain reactions Yusuke Sako, Jumpei Morimoto, Hiroshi Murakami and Hiroaki Suga. J Am Chem Soc. 2008 Jun 11;130(23):7232-4. https://doi.org/10.1021/ja800953c
2008 Citation Comprehensive analysis of the effects of Escherichia coli ORFs on protein translation reaction Yasuaki Kazuta, Jiro Adachi, Tomoaki Matsuura, Naoaki Ono, Hirotada Mori and Tetsuya Yomo. Mol Cell Proteomics. 2008 Aug;7(8):1530-40. https://doi...
2008 Citation A general and rapid cell-free approach for the interrogation of protein-protein, protein-DNA, and protein-RNA interactions and their antagonists utilizing split-protein reporters Jason R Porter, Cliff I Stains, Benjamin W Jester and Indraneel Ghosh. J Am Chem Soc. 2008 May 21;130(20):6488-97. https://doi.org/10.1021/ja7114579
2008 Citation Molecular mechanism of drug-dependent ribosome stalling Nora Vazquez-Laslop, Celine Thum and Alexander S Mankin. Mol Cell. 2008 Apr 25;30(2):190-202. https://doi.org/10.1016/j.molcel.2008.02.026
2008 Citation Ribosomal synthesis of N-methyl peptides Alexander O Subtelny, Matthew C T Hartman and Jack W Szostak. J Am Chem Soc. 2008 May 14;130(19):6131-6. https://doi.org/10.1021/ja710016v
2008 Citation Fluorescence labeling of a cytokine with desthiobiotin-tagged fluorescent puromycin Akihiko Sakamoto, Mai Yamagishi, Takafumi Watanabe, Youichi Aizawa, Takashi Kato and Takashi Funatsu. J Biosci Bioeng. 2008 Mar;105(3):238-42. htt...
2008 Citation Messenger RNA-programmed incorporation of multiple N-methyl-amino acids into linear and cyclic peptides Takashi Kawakami, Hiroshi Murakami and Hiroaki Suga. Chem Biol. 2008 Jan;15(1):32-42. https://doi.org/10.1016/j.chembiol.2007.12.008
2008 Citation Reprogramming the translation initiation for the synthesis of physiologically stable cyclic peptides Yuki Goto, Atsushi Ohta, Yusuke Sako, Yusuke Yamagishi, Hiroshi Murakami and Hiroaki Suga. ACS Chem Biol. 2008 Feb 15;3(2):120-9. https://doi.org/...
2008 Citation UncI protein can mediate ring-assembly of c-subunits of FoF1-ATP synthase in vitro Yoko Ozaki, Toshiharu Suzuki, Yutetsu Kuruma, Takuya Ueda and Masasuke Yoshida. Biochem Biophys Res Commun. 2008 Mar 14;367(3):663-6. https://doi....
2008 Citation Compensatory evolution of a WW domain variant lacking the strictly conserved Trp residue Hayato Yanagida, Tomoaki Matsuura and Tetsuya Yomo. J Mol Evol. 2008 Jan;66(1):61-71. https://doi.org/10.1007/s00239-007-9061-5
2007 Citation Synthesis of polyester by means of genetic code reprogramming Atsushi Ohta, Hiroshi Murakami, Eri Higashimura and Hiroaki Suga. Chem Biol. 2007 Dec;14(12):1315-22. https://doi.org/10.1016/j.chembiol.2007.10.015
2007 Citation A small RNA regulates multiple ABC transporter mRNAs by targeting C/A-rich elements inside and upstream of ribosome-binding sites Cynthia M Sharma, Fabien Darfeuille, Titia H Plantinga and Jörg Vogel. Genes Dev. 2007 Nov 1;21(21):2804-17. https://doi.org/10.1101/gad.447207
2007 Citation Elongation factor Tu mutants expand amino acid tolerance of protein biosynthesis system Yoshio Doi, Takashi Ohtsuki, Yoshihiro Shimizu, Takuya Ueda and Masahiko Sisido. J Am Chem Soc. 2007 Nov 21;129(46):14458-62. https://doi.org/10.1...
2007 Citation An mRNA-protein fusion at N-terminus for evolutionary protein engineering Shingo Ueno, Hidenao Arai, Miho Suzuki and Yuzuru Husimi. Int J Biol Sci. 2007 Aug 29;3(6):365-74. https://doi.org/10.7150/ijbs.3.365
2007 Citation Protein synthesis in liposomes with a minimal set of enzymes Giovanni Murtas, Yutetsu Kuruma, Paolo Bianchini, Alberto Diaspro and Pier Luigi Luisi. Biochem Biophys Res Commun. 2007 Nov 9;363(1):12-7. https:...
2007 Citation Oxidation of elongation factor G inhibits the synthesis of the D1 protein of photosystem II Kouji Kojima, Masaru Oshita, Yohei Nanjo, Koji Kasai, Yuzuru Tozawa, Hidenori Hayashi and Yoshitaka Nishiyama. Mol Microbiol. 2007 Aug;65(4):936-47...
2007 Citation Structural and functional definition of the specificity of a novel caspase-3 inhibitor, Ac-DNLD-CHO Atsushi Yoshimori, Junichi Sakai, Satoshi Sunaga, Takanobu Kobayashi, Satoshi Takahashi, Naoyuki Okita, Ryoko Takasawa and Sei-ichi Tanuma. BMC Pha...
2007 Citation Selection of restriction endonucleases using artificial cells Yu Zheng and Richard J Roberts. Nucleic Acids Res. 2007;35(11):e83. https://doi.org/10.1093/nar/gkm410
2007 Citation Cis-regulatory hairpin-shaped mRNA encoding a reporter protein: catalytic sensing of nucleic acid sequence at single nucleotide resolution Atsushi Narita, Kazumasa Ogawa, Shinsuke Sando and Yasuhiro Aoyama. Nat Protoc. 2007;2(5):1105-16. https://doi.org/10.1038/nprot.2007.140
2007 Citation Unexpected pCitationerence of the E. coli translation system for the ester bond during incorporation of backbone-elongated substrates Shinsuke Sando, Kenji Abe, Nobuhiko Sato, Toshihiro Shibata, Keigo Mizusawa and Yasuhiro Aoyama. J Am Chem Soc. 2007 May 16;129(19):6180-6. https:...
2007 Citation Ribosomal protein S1 influences trans-translation in vitro and in vivo Matthieu Saguy, Reynald Gillet, Patricia Skorski, Sylvie Hermann-Le Denmat and Brice Felden. Nucleic Acids Res. 2007;35(7):2368-76. https://doi.or...
2007 Citation Identification of nascent chain interaction sites on trigger factor Sathish K Lakshmipathy, Sladjana Tomic, Christian M Kaiser, Hung-Chun Chang, Pierre Genevaux, Costa Georgopoulos, José M Barral, Arthur E Johnson, ...
2007 Citation Nascent chain, mRNA, and ribosome complexes generated by a pure translation system Tomoaki Matsuura, Hayato Yanagida, Junya Ushioda, Itaru Urabe and Tetsuya Yomo. Biochem Biophys Res Commun. 2007 Jan 12;352(2):372-7. https://doi....
2007 Citation Efficient protein selection based on ribosome display system with purified components Hiroyuki Ohashi, Yoshihiro Shimizu, Bei-Wen Ying and Takuya Ueda. Biochem Biophys Res Commun. 2007 Jan 5;352(1):270-6. https://doi.org/10.1016/j.b...
2006 Citation Cytosolic factor- and TOM-independent import of C-tail-anchored mitochondrial outer membrane proteins Kiyoko Setoguchi, Hidenori Otera and Katsuyoshi Mihara. EMBO J. 2006 Dec 13;25(24):5635-47. https://doi.org/10.1038/sj.emboj.7601438
2006 Citation Femtoliter compartment in liposomes for in vitro selection of proteins Takeshi Sunami, Kanetomo Sato, Tomoaki Matsuura, Koji Tsukada, Itaru Urabe and Tetsuya Yomo. Anal Biochem. 2006 Oct 1;357(1):128-36. https://doi.o...
2006 Citation Affinity maturation of phage display antibody populations using ribosome display Maria Groves, Steven Lane, Julie Douthwaite, David Lowne, D Gareth Rees, Bryan Edwards and Ronald H Jackson. J Immunol Methods. 2006 Jun 30;313(1-2...
2006 Citation Regulation of mitochondrial morphology through proteolytic cleavage of OPA1 Naotada Ishihara, Yuu Fujita, Toshihiko Oka and Katsuyoshi Mihara. EMBO J. 2006 Jul 12;25(13):2966-77. https://doi.org/10.1038/sj.emboj.7601184
2006 Citation Ribosomal synthesis of dehydroalanine-containing peptides Florian P Seebeck and Jack W Szostak. J Am Chem Soc. 2006 Jun 7;128(22):7150-1. https://doi.org/10.1021/ja060966w
2006 Citation Co-translational binding of GroEL to nascent polypeptides is followed by post-translational encapsulation by GroES to mediate protein folding Bei-Wen Ying, Hideki Taguchi and Takuya Ueda. J Biol Chem. 2006 Aug 4;281(31):21813-9. https://doi.org/10.1074/jbc.m603091200
2006 Citation Mass spectrometry of hydrogen/deuterium exchange in 70S ribosomal proteins from E. coli Tatsuya Yamamoto, Shunsuke Izumi and Kunihiko Gekko. FEBS Lett. 2006 Jun 26;580(15):3638-42. https://doi.org/10.1016/j.febslet.2006.05.049
2006 Citation Cytosolic chaperonin protects folding intermediates of Gbeta from aggregation by recognizing hydrophobic beta-strands Susumu Kubota, Hiroshi Kubota and Kazuhiro Nagata. Proc Natl Acad Sci U S A. 2006 May 30;103(22):8360-5. https://doi.org/10.1073/pnas.0600195103
2006 Citation Genetically encoded but nonpolypeptide prolyl-tRNA functions in the A site for SecM-mediated ribosomal stall Hiroki Muto, Hitoshi Nakatogawa and Koreaki Ito. Mol Cell. 2006 May 19;22(4):545-52. https://doi.org/10.1016/j.molcel.2006.03.033
2006 Citation Highly efficient ribosome display selection by use of purified components for in vitro translation Denis Villemagne, Ronald Jackson and Julie A Douthwaite. J Immunol Methods. 2006 Jun 30;313(1-2):140-8. https://doi.org/10.1016/j.jim.2006.04.001
2006 Citation A highly flexible tRNA acylation method for non-natural polypeptide synthesis Hiroshi Murakami, Atsushi Ohta, Hiroshi Ashigai and Hiroaki Suga. Nat Methods. 2006 May;3(5):357-9. https://doi.org/10.1038/nmeth877
2006 Citation Small protein B interacts with the large and the small subunits of a stalled ribosome during trans-translation Marc Hallier, Jessie Desreac and Brice Felden. Nucleic Acids Res. 2006 Apr 12;34(6):1935-43. https://doi.org/10.1093/nar/gkl097
2006 Citation SmpB triggers GTP hydrolysis of elongation factor Tu on ribosomes by compensating for the lack of codon-anticodon interaction during trans-translation initiation Yoshihiro Shimizu and Takuya Ueda. J Biol Chem. 2006 Jun 9;281(23):15987-96. https://doi.org/10.1074/jbc.m512165200
2006 Citation Enhancement of protein synthesis by an inorganic polyphosphate in an E. coli cell-free system Hiromichi Itoh, Yumi Kawazoe and Toshikazu Shiba. J Microbiol Methods. 2006 Feb;64(2):241-9. https://doi.org/10.1016/j.mimet.2005.05.003
2006 Citation Termination-free prokaryotic protein translation by using anticodon-adjusted E. coli tRNASer as unified suppressors of the UAA/UGA/UAG stop codons. Read-through ribosome display of full-length DHFR with translated UTR as a buried spacer arm Atsushi Ogawa, Shinsuke Sando and Yasuhiro Aoyama. Chembiochem. 2006 Feb;7(2):249-52. https://doi.org/10.1002/cbic.200500397
2006 Citation Spermidine inhibits transient and stable ribosome subunit dissociation So Umekage and Takuya Ueda. FEBS Lett. 2006 Feb 20;580(5):1222-6. https://doi.org/10.1016/j.febslet.2006.01.033
2006 Citation Exploring the capacity of trigger factor to function as a shield for ribosome bound polypeptide chains Sladjana Tomic, Arthur E Johnson, F Ulrich Hartl and Stephanie A Etchells. FEBS Lett. 2006 Jan 9;580(1):72-6. https://doi.org/10.1016/j.febslet.20...
2005 Citation Ribosomal synthesis of unnatural peptides Kristopher Josephson, Matthew C T Hartman and Jack W Szostak. J Am Chem Soc. 2005 Aug 24;127(33):11727-35. https://doi.org/10.1021/ja0515809
2005 Citation Protein synthesis by pure translation systems Yoshihiro Shimizu, Takashi Kanamori and Takuya Ueda. Methods. 2005 Jul;36(3):299-304. https://doi.org/10.1016/j.ymeth.2005.04.006
2005 Citation Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins Yutetsu Kuruma, Ken-Ichi Nishiyama, Yoshihiro Shimizu, Matthias Müller and Takuya Ueda. Biotechnol Prog. Jul-Aug 2005;21(4):1243-51. https://doi.o...
2005 Citation A small-molecule-based approach to sense codon-templated natural-unnatural hybrid peptides. Selective silencing and reassignment of the sense codon by orthogonal reacylation stalling at the single-codon level Shinsuke Sando, Keiichiro Kanatani, Nobuhiko Sato, Hironori Matsumoto, Takahiro Hohsaka and Yasuhiro Aoyama. J Am Chem Soc. 2005 Jun 8;127(22):7998...
2005 Citation Functional role played by the glycosylphosphatidylinositol anchor glycan of CD48 in interleukin-18-induced interferon-gamma production Keiko Fukushima, Yukio Ikehara and Katsuko Yamashita. J Biol Chem. 2005 May 6;280(18):18056-62. https://doi.org/10.1074/jbc.m413297200
2005 Citation Involvement of Hu and heterogeneous nuclear ribonucleoprotein K in neuronal differentiation through p21 mRNA post-transcriptional regulation Masato Yano, Hirotaka J Okano and Hideyuki Okano. J Biol Chem. 2005 Apr 1;280(13):12690-9. https://doi.org/10.1074/jbc.m411119200
2005 Citation Co-translational involvement of the chaperonin GroEL in the folding of newly translated polypeptides Bei-Wen Ying, Hideki Taguchi, Mayumi Kondo and Takuya Ueda. J Biol Chem. 2005 Mar 25;280(12):12035-40. https://doi.org/10.1074/jbc.m500364200
2005 Citation Novel processing and localization of catA, ccdA associated thiol-disulfide oxidoreductase, in protein hyper-producing bacterium Brevibacillus choshinensis Ryoichi Tanaka, Makoto Mizukami and Masao Tokunaga. Protein Pept Lett. 2005 Jan;12(1):95-8. https://doi.org/10.2174/0929866053406093
2004  Citation Chaperone-assisted folding of a single-chain antibody in a reconstituted translation system Bei-Wen Ying, Hideki Taguchi, Hiroshi Ueda and Takuya Ueda. Biochem Biophys Res Commun. 2004 Aug 6;320(4):1359-64. https://doi.org/10.1016/j.bbrc....
2004  Citation Bulge- and basal layer-specific expression of fibroblast growth factor-13 (FHF-2) in mouse skin Mitsuko Kawano, Satoshi Suzuki, Masashi Suzuki, Junko Oki and Toru Imamura. J Invest Dermatol. 2004 May;122(5):1084-90. https://doi.org/10.1111/j....
2004  Citation Evidence for the translation initiation of leaderless mRNAs by the intact 70 S ribosome without its dissociation into subunits in eubacteria Tsuyoshi Udagawa, Yoshihiro Shimizu and Takuya Ueda. J Biol Chem. 2004 Mar 5;279(10):8539-46. https://doi.org/10.1074/jbc.m308784200
2004  Citation Reinvestigation of the requirement of cytosolic ATP for mitochondrial protein import Takeyoshi Asai, Takashi Takahashi, Masatoshi Esaki, Shuh-ichi Nishikawa, Kenzo Ohtsuka, Masato Nakai and Toshiya Endo. J Biol Chem. 2004 May 7;279(...
2003 Citation A novel screening system for self-mRNA targeting proteins Bei-Wen Ying, Tsutomu Suzuki, Yoshihiro Shimizu and Takuya Ueda. J Biochem. 2003 Apr;133(4):485-91. https://doi.org/10.1093/jb/mvg064
2001 Citation https://doi.org/10.1038/90802 Y Shimizu, A Inoue, Y Tomari, T Suzuki, T Yokogawa, K Nishikawa and T Ueda.