this page: Exosome Dashboard
Product Lineup
Tetraspanin mAbs for exosome purification and characterization
Tetraspanin antibody-based exosome purification and ELISA kit
ExoTrap™(to capture exosomes)
Ultracentrifugation media for exosome purification
OptiPrep™ (for exosome isolation)
Milk exosome reagents
Antibodies and ELISAs for putative exosome markers and negative controls
Other Resources
User Reports
Annotated Bibliography
ExoTrap™ Spin Column
CosmoBio USA's ExoTrap™ Exosome Isolation Spin Column Kit uses spin column-immobilized anti human CD9 antibody (clone 12A12) to capture exosomes. Exosome-derived proteins and micro RNAs can be isolated within 30 minutes from human serum, plasma, saliva, urine and culture supernatants.
Features
- Recover exosomes from serum, plasma, urine, saliva, culture supernatant
- High purity exosomes within 30 minutes
- Easy spin column operation
Experimental examples
Using ExoTrap™ exosomes were recovered in 50ul SDS sample buffer. Exosome marker expression was analyzed by western blot.
Figure 1. Exosome isolation from culture supernatant. 293T cell culture supernatant and MKN7 cell culture supernatant (20ul/lane) was probed with: Anti-CD9 (clone 12A12), Anti-CD63 (clone 8A12) and Anti-CD81 (clone 12C4).
Figure 2. Exosome isolation from human plasma. EDTA-treated human plasma (20 ul/lane) probed with anti-CD9 (clone 12A12).
Click for Exotrap™ User Reports
Ordering Information
Product | Clone | Label | Unit size | Catalog Number |
---|---|---|---|---|
Tetraspanin antibody-based exosome purification and ELISA kit | ||||
ExoTrap™ Exosome Isolation Spin Column Kit | 12A12 | - | 10 preps | CSR-SHI-EXO-K010 |