Magnetic Cell Separation Kit for

Human CD44v9+ Cancer Stem Cells

Enrichment of cancer stem cells is easily possible

The Magnetic Cell Separation Kit for Human CD44v9+ Cancer Stem Cells is a magnetic particle conjugated with a monoclonal antibody that binds with high affinity to the membrane antigen CD44v8-10, which is highly expressed on cancer stem cells. Cancer stem cells can be enriched.

Background

In recent years, it has become clear that cancer stem cells are the root cause of canceration, such as the formation of cancer tissue, its metastasis, and recurrence. Therefore, it is considered essential to eliminate cancer stem cells in order to eradicate cancer, and cancer stem cells are attracting attention as the ultimate target for cancer therapy. However, since cancer stem cells exist in an extremely small amount of less than a few percent in tissues and cell lines, it has been difficult to develop therapeutic drugs targeting them and to analyze their functions.
"Anti-CD44v antibody" is a monoclonal antibody that binds with high affinity to the membrane antigen CD44v8-10, which is highly expressed in cancer stem cells. concentration becomes easily achievable. By concentrating cancer stem cells, it is possible to construct a "sphere culture system" used as an in vitro evaluation system for cancer stem cells and a "mouse lung metastasis model" used as an in vivo evaluation system. This makes it possible to elucidate the mechanism of cancer development and metastasis, and to screen therapeutic drugs for cancer stem cells. "Anti-CD44v antibody" will be a powerful tool for the development of new therapeutic drugs targeting cancer stem cells.

Features

• Magnetic particles conjugated with cancer stem cell marker antibodies can easily enrich cancer stem cells
• Harvesting cancer stem cells with simple operation using a magnetic stand without using FACS
• Separated cells can be used for flow cytometric analysis

Composition

• anti-CD44v9 magnetic beads (RV3 beads)
• Binding buffer
• Wash buffer

Workflow of Cancer Stem Cell Enrichment Kit

Experimental example using flow cytometry

Cells stably expressing CD44v9-GFP were isolated and analyzed by flow cytometry

Fig. 1. Ratio of CD44v9-expressing cells among RV3 beads-positive cells Cell groups in which 293F cells stably expressing
CD44v9-GFP and parental cell line 293F cells were mixed at an arbitrary ratio (A, B, C). was separated using this product.
CD44v9-expressing cells were enriched from 4.97% (A), 15.3% (B), 55.7% (C) before isolation to 86.4% (A), 97.1% (B), 99.0% (C) after isolation. rice field.

Isolate cancer cells and analyze by flow cytometry

Figure 2. Ratio of CD44v9-expressing cells in
RV3 bead-positive cells RV3 bead-positive cells were stained with PE-labeled anti-rat IgG antibody and analyzed by flow cytometry. In human prostate cancer cell line PC-3 (A) and human pancreatic adenocarcinoma cell line AsPC-1 (B), the ratio of CD44v9-expressing cells also increased compared to before isolation.

References

[1] Tanabe KK., et al., Lancet 1993; 341: 725–726. PMID: 8095628
[2] Nagano O., et al., Oncogene 21 January 2013, 1-8. PMID:23334333
[3] Ishimoto T., et al., Cancer Cell 2011; 19: 387–400. PMID : 21397861
[4] Tsugawa H., et al., Cell Host Microbe 2012 ; 12: 764-777. PMID: 23245321
[5] Yae T., et al., Nat Commun 2012; 3: 883: 1-9. PMID: 22673910

Magnetic Cell Separation Kit for Human CD44v9+ Cancer Stem Cells

Catalog Number	Product Name	Size
CSR-CSC-SEP1	CD44v9+ Human Cancer Stem Cell Magnetic Isolation Kit	10 rxns