E-RDF Medium

Cosmo Bio LTD

Catalog No: KYO-26500

See more products for microbial growth and research at the Microbial Research Dashboard.

E-RDF Medium is a basic synthetic serum-free medium for culture of  human and mouse hybridoma cell lines including for example,  NS-1, MPC-11, MCB-1. Well suited for monoclonal antibody production by high density, large scale culture. Contact us for customized formulations. Product Cat. No. KYO-26500 contains sufficient material to prepare 10 Liters of medium.

E-RDF Medium is an improved, "Enriched" version of RDF medium ( RPMI 1640:DMEM:HAMF12, (2:1:1))

Storage conditions: Dark place / moisture proof, 2-10 ℃
Validity period: 2 years

Preparation of E-RDF Medium (for 1 Liter)
(1) Dissolve 17.7 g of E-RDF Medium powder in approximately 800 mL of ultrapure water.
(2) Add 1,130 mg sodium hydrogen carbonate, dissolve and adjust volume to 1000 mL.
(3) Immediately sterilize the prepared medium solution in a sterilized medium bottle with a membrane filter (0.22 μm).
(4) Please use immediately after preparing the medium. When storing, please store under refrigerated conditions.
(5) When using, aseptically add the additive RD-1 as necessary before use.

If desired, "RD-1 Supplement for E-RDF Medium" (#KYO-20300) is also available. RD-1 Supplement for E-RDF Medium contains:
Insulin: 5 mg/L
Transferrin: 10 mg/L
Ethanolamine: 1.53 mg/L
Sodium selenite: (non-medicinal toxicant) 0.0043 mg/L
Excipients

Recent Literature use-citations for E-RDF Medium (cited in more than 300 publications) 

• Anti-nanodisc antibodies specifically capture nanodiscs and facilitate molecular interaction kinetics studies for membrane protein.,F Nakagawa, M Kikkawa, S Chen, Y Miyashita., Sci Rep., 2023
• Expression and functional analyzes for estrogen receptor and estrogen-related receptor of Yesso scallop, Patinopecten yessoensis., W Gu, T Thitiphuree, Y Otoki, EC Marquez., J Steroid Biochem Mol., 2023
• Quality assessment method for Chinpi, dried Citrus spp. peel and its derived Kampo medicines using specific monoclonal antibody against hesperidin., K Noguchi, D Imahori, Y Kido., Phytochem Anal. ., 2023
• Cellular and molecular mechanisms of NiONPs toxicity on eel hepatocytes HEPAE1: An illustration of the impact of Ni release from mining activity in New., O Germande, F Beaufils, G Daffe, P Gonzalez., Chemosphere., 2022
• Development of Highly Sensitive Chemiluminescence Enzyme Immunostaining Assay to Determine Glycyrrhizin Content Using Anti-glycyrrhizin Monoclonal Antibody., S Fujii, Y Shoyama, S Nomura, T Uto., Chem Pharm Bull (Tokyo)., 2022
• Blockade of the interaction between BMP9 and endoglin on erythroid progenitors promotes erythropoiesis in mice., A Yamaguchi, I Hirano, S Narusawa, K Shimizu., Genes Cells., 2021
• Class-Switching of B Lymphocytes by DNA and Cell Immunization for Stereospecific Monoclonal Antibodies against Native GPCR., Y Isozaki, K Tsumoto, M Tomita., Immuno., 2021
• Conformation-specific monoclonal antibodies recognizing the native structure of G protein-coupled receptor (GPCR)., Y Isozaki, K Tsumoto, M Tomita., International Immunopharmacology., 2021
• Immunomodulatory effect of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in allergic conditions in vitro and in vivo., A Iwamoto, Y Inoue, H Tachibana, H Kawahara., Cytotechnology., 2021
• Transcriptional Regulation of Müllerian Inhibiting Substance (MIS) and Establishment of a Gonadal Somatic Cell Line Using mis-GFP Transgenic Medaka (Oryzias latipes). Kawabe T, Kariya H, Hara S, Shirozu T, Shiraishi E, Mukai K, Yazawa T, Inoue S, Kitano T. Front Endocrinol (Lausanne)., 2020
• Alkalisoluble pectin suppresses IgE production in human myeloma cell line in vitro., A Iwamoto, Y Inoue, H Tachibana, H Kawahara., Cytotechnology., 2019
• Paralogs of common carp granulocyte colony-stimulating factor (G-CSF) have different functions regarding development, trafficking and activation., F Katakura, K Nishiya, AS Wentzel, E Hino., Front Immunol., 2019
• Vertebrates originally possess four functional subtypes of G proteincoupled melatonin receptor., K Sakai, Y Yamamoto, T Ikeuchi., Scientific Reports., 2019
• Sensitive quantitative analysis of the bitter glycoside amarogentin by specific monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay. Sakamoto S, Wada S, Tanaka H, ​​Morimoto S. RSC Adv. 2018 
• Analysis of antigen recognition mechanism of anti-paclitaxel monoclonal antibody. Kaori Tabata, Hideki Arai, Hiroyuki Tanaka, Taro Tamada, Satoshi Morimoto, Ryota Kuroki. Collection of SPring-8/SACLA utilization research results. 2017
• Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9.Nakakita S, Itoh A, Nakakita Y, Nonaka Y, Ogawa T, Nakamura T, Nishi N., J Biol Chem. 2016
• Detection of Ganoderic Acid A in Ganoderma lingzhi by an Indirect Competitive Enzyme-Linked Immunosorbent Assay. Sakamoto S, Kohno T, Shimizu K, Tanaka H, ​​Morimoto S., Planta Med. 2016
• Recombinant carp IL-4/13B stimulates in vitro proliferation of carp IgM(+) B cells. Yamaguchi T, Miyata S, Katakura F, Nagasawa T, Shibasaki Y, Yabu T, Fischer U, Nakayasu C, Nakanishi T, Moritomo T. , Fish Shellfish Immunol. 2016