Deoxynivalenol(DON) ELISA Kit

Nittobo Medical

Catalog No.: NMD-MSFR078020

Kit for mycotoxin measurement

Background

Deoxynivalenol (DON) is a type of trichothecene mycotoxin produced by Fusarium fungi, known as the cause of red mold disease in wheat and other crops. Ingestion of grains contaminated with DON is said to cause severe poisoning symptoms in humans and livestock.
In Japan, the provisional standard value for DON in wheat has been set at 1.1 ppm, and the provisional tolerance value for DON in feed has been set at 4 ppm or less (for cattle aged 3 months or older).

Features

• Measures deoxynivalenol (DON)
  Deoxynivalenol (DON) remaining in wheat can be measured and quantified.
• Silage can also be measured.
  It is now possible to measure fermented feed such as silage, which was previously difficult to measure.
• High sensitivity:
  Detects up to the ppb level, and can be measured on par with analytical instruments

Recovery test using wheat

Cross-reactivity with other trichothecene mycotoxins

DON	100%
15Ac-DON	120%
3Ac-DON	0.1%
NIV	5%
T2 Toxin	0.1%

Kit Components

• IgG immobilized plate (strip type)
• DON standard (prepared)
• Label solution (HRP labeled DON)
• Labeled dilution solution
• antibody solution
• enzyme substrate solution
• Stop solution
• Concentrated cleaning solution

Measurement principle

This ELISA kit is a direct competitive reaction assay using an antibody that specifically recognizes DON. Anti-mouse IgG capture antibody is immobilized in each well of the plate. A competitive reaction occurs by sequentially adding the reagents in the kit and a sample to each well. After one hour of reaction, an HRP-bound DON-antibody complex is formed. The amount of this complex depends on the amount of DON contained in the sample to be measured. The DON concentration in the sample can then be determined by measuring the enzyme (HRP) activity of this complex.