Anti CD90 mAb (Clone: A6-10G9)

Hakarel

Catalog No.: HAK-ANTI-CD90-A6-10G9

Product Description

Exosomes are small vesicles with diameters of 30 to 200 nm that are secreted by almost all cells in the body and are present in all body fluids, including blood and urine^(1)-(5). They are also secreted in vitro into the culture supernatant of animal cells. Like cells, exosomes are enclosed in a lipid bilayer membrane with membrane proteins on their surface and proteins and microRNAs encapsulated inside. These components are believed to enable exosomes to mediate intercellular communication by affecting target cells that have taken them up⁽⁶⁾. A key structural feature of exosomes is the presence of tetraspanin family proteins such as CD9, CD63, and CD81, which are considered surface markers for exosomes⁽⁷⁾.

Human mesenchymal stem cells (hMSCs) are pluripotent cells with tissue regeneration and immunoregulatory capabilities, making them valuable for cell therapy. Recent studies suggest that the beneficial effects of hMSCs may be due in part to paracrine signaling mediated by extracellular vesicles such as exosomes^{(8), (9)}.

Exosome quantification methods include measuring protein content and particle analysis using nanotracking⁽¹⁰⁾, but these require purified exosomes obtained via ultracentrifugation. Currently, no general method exists to directly quantify exosomes in body fluids or culture media.

This kit is a two-step sandwich ELISA that detects CD90-positive and CD63-positive exosomes secreted by human MSCs. It uses high-performance antibodies targeting CD63—the most highly expressed tetraspanin in hMSCs—and CD90, a proposed marker for MSC-derived exosomes⁽¹¹⁾.

Features

• Direct quantification of CD90+CD63+ exosomes in human cell culture supernatants and other samples.
• Compatible with standard plate readers (450 nm); no special equipment needed.
• Stability and reproducibility ensured by using a CD90/CD63 fusion protein as a standard instead of exosomes.
• Relative quantification possible via standard curve generated with CD90/CD63 fusion protein.
• Two-step sandwich ELISA method using immobilized anti-CD90 and HRP-labeled anti-CD63 antibodies.

What's Included

#	Contents	Capacity	Quantity
1	Anti-CD90 antibody immobilized plate	96 wells (8 × 12 strips)	1 sheet
2	Standard protein (CD90/CD63 fusion)	200 µL	1 pc*
3	Assay Buffer	25 mL	1 bottle
4	Washing Buffer (10×)	25 mL	1 bottle
5	HRP-labeled anti-CD63 antibody (500×)	20 µL	1 bottle
6	Substrate solution	12 mL	1 bottle
7	Stop solution (2N H2SO4)	6 mL	1 bottle

Product Specifications

• Application: ELISA, WB
• Clonality: Monoclonal IgG1/κ (Clone No.: A6-10G9)
• Host: Mouse
• Source: Human
• Conjugation: Unlabeled