Afamin/Wnt3a CM (High Concentration)

MBL Life Sciences

Catalog No.: MBL-J2-005-05

High-potency Wnt3a signaling in a serum-free, defined formulation

Afamin/Wnt3a CM (High Concentration) is a premium, ready-to-use conditioned medium designed to deliver robust and sustained activation of the canonical Wnt signaling pathway. This high-activity formulation features a stabilized complex of mouse Wnt3a and human Afamin, co-expressed in Expi293F™ cells, providing enhanced solubility, bioactivity, and long-term performance in defined culture systems.

Key Benefits

• High Wnt3a Activity – Enables lower working concentrations (recommended 2.5% v/v)
• Improved Stability – Afamin complex prevents Wnt3a precipitation in defined media
• Consistent Performance – Supports long-term organoid culture without serum
• Optimized Expression System – Produced in Expi293F™ for high yield and bioactivity
• Sterile & Defined – 0.2 μm filtered; endotoxin level <1.0 EU/mL

Applications

• Organoid culture from intestine, stomach, pancreas, and liver
• Long-term expansion of Lgr5+ intestinal stem cells
• Serum-free, reproducible stem cell and tissue modeling workflows

Storage & Handling

Store at –20°C or below. Aliquot upon first thaw to prevent activity loss from freeze–thaw cycles.

Note: Use in combination with other factors may be subject to third-party patents. Please consult your institution’s legal office for guidance.

Product Specifications

• Application: Cell Culture
• Endotoxin Levels: < 1.0 EU/mL by the LAL assay