2-Deoxyglucose (2DG) Uptake Measurement Kit

Cosmo Bio

SKU: CSR-OKP-PMG-K01H

Background

2-deoxyglucose uptake measurement is a reliable approach for estimating glucose uptake in cells and tissues. Based on a published method, the 2-Deoxyglucose (2DG) Uptake Measurement Kit facilitates highly sensitive and direct enzymatic 2DG quantitation, obviating the need for radioisotopes and their attendant issues.

Measurement of 2-deoxyglucose (2DG) uptake in tissues and cells is a reliable approach for estimating glucose uptake and thereby to explore the regulation of glucose metabolism and mechanisms of insulin resistance. While assays employing radioisotope-labeled 2DG are often used to measure 2DG uptake in vivo and in vitro, the use of radioisotopes is not possible without problems including permitting, handling, and disposal. Furthermore, in actual practice, radioactive assay protocols for 2DG uptake require a corrective separation step to account for labeled 2DG that remains in extracellular fluids and can lead to substantial variation. Such problems are obviated by the enzymatic method of 2DG detection employed by this kit, based on published methods (Saito K and Minokoshi Y, et al. Analytical Biochem 412: 9-17, 2011).

Features

• No need for radioisotopes (RIs) or radiation counting instrumentation
• Photometric readout on standard microplate readers
• Direct measurement of 2DG6P accumulation in cells
• High sensitivity achieved by a recycling enzymatic amplification reaction

Assay principle in five key steps

1. Oxidation of glucose-6-phosphate (G6P) with a low concentration of G6P dehydrogenase (G6PDH) with NAD+ to eliminate endogenous G6P in target cells
2. Elimination of NAD(P)H with HCl, which removes endogenous NAD(P)H as well as NADH produced in step 1
3. Generation of NADPH through oxidation of 2DG6P in cells with a high concentration of G6PDH, the generated NADPH being used for quantification of 2DG6P
4. Elimination of NAD(P)+ and G6PDH that remains after Step 2 with NaOH
5. Recycling amplification reaction for the small amount of NADPH generated, and quantification of 2DG6P using a photometric microplate reader at 420 nanometers wavelength

Citations for 2-Deoxyglucose (2DG) Uptake Measurement Kit – 29 Found

Kunii, Masataka; Ohara-Imaizumi, Mica; Takahashi, Noriko; Kobayashi, Masaki; Kawakami, Ryosuke; Kondoh, Yasumitsu; Shimizu, Takeshi; Simizu, Siro; Lin, Bangzhong; Nunomura, Kazuto; Aoyagi, Kyota; Ohno, Mitsuyo; Ohmuraya, Masaki; Sato, Takashi; Yoshimura, Shin-Ichiro; Sato, Ken; Harada, Reiko; Kim, Yoon-Jeong; Osada, Hiroyuki; Nemoto, Tomomi; Kasai, Haruo; Kitamura, Tadahiro; Nagamatsu, Shinya; Harada, Akihiro. Opposing roles for SNAP23 in secretion in exocrine and endocrine pancreatic cells. The Journal Of Cell Biology. 2016;215(1):121-138. 

Ijuin, Takeshi; Hosooka, Tetsuya; Takenawa, Tadaomi. Phosphatidylinositol 3,4,5-Trisphosphate Phosphatase SKIP Links Endoplasmic Reticulum Stress in Skeletal Muscle to Insulin Resistance. Molecular And Cellular Biology. 2016;36(1):108-18. 

Albert, Verena; Svensson, Kristoffer; Shimobayashi, Mitsugu; Colombi, Marco; Muñoz, Sergio; Jimenez, Veronica; Handschin, Christoph; Bosch, Fatima; Hall, Michael N. mTORC2 sustains thermogenesis via Akt-induced glucose uptake and glycolysis in brown adipose tissue. Embo Molecular Medicine. 2016;8(3):232-46. 

Chee, Carolyn; Shannon, Chris E; Burns, Aisling; Selby, Anna L; Wilkinson, Daniel; Smith, Kenneth; Greenhaff, Paul L; Stephens, Francis B. Relative Contribution of Intramyocellular Lipid to Whole-Body Fat Oxidation Is Reduced With Age but Subsarcolemmal Lipid Accumulation and Insulin Resistance Are Only Associated With Overweight Individuals. Diabetes. 2016;65(4):840-50. 

Uruno, Akira; Yagishita, Yoko; Katsuoka, Fumiki; Kitajima, Yasuo; Nunomiya, Aki; Nagatomi, Ryoichi; Pi, Jingbo; Biswal, Shyam S; Yamamoto, Masayuki. Nrf2-Mediated Regulation of Skeletal Muscle Glycogen Metabolism. Molecular And Cellular Biology. 2016;36(11):1655-72. 

Guridi, Maitea; Tintignac, Lionel A; Lin, Shuo; Kupr, Barbara; Castets, Perrine; Rüegg, Markus A. Activation of mTORC1 in skeletal muscle regulates whole-body metabolism through FGF21. Science Signaling. 2015;8(402):ra113. 

Tamura, Yukinori; Kawao, Naoyuki; Yano, Masato; Okada, Kiyotaka; Okumoto, Katsumi; Chiba, Yasutaka; Matsuo, Osamu; Kaji, Hiroshi. Role of plasminogen activator inhibitor-1 in glucocorticoid-induced diabetes and osteopenia in mice. Diabetes. 2015;64(6):2194-206. 

Jayakody, Lahiru N; Kadowaki, Masafumi; Tsuge, Keisuke; Horie, Kenta; Suzuki, Akihiro; Hayashi, Nobuyuki; Kitagaki, Hiroshi. SUMO expression shortens the lag phase of Saccharomyces cerevisiae yeast growth caused by complex interactive effects of major mixed fermentation inhibitors found in hot-compressed water-treated lignocellulosic hydrolysate. Applied Microbiology And Biotechnology. 2015;99(1):501-15. 

Ijuin, Takeshi; Takenawa, Tadaomi. Improvement of insulin signaling in myoblast cells by an addition of SKIP-binding peptide within Pak1 kinase domain. Biochemical And Biophysical Research Communications. 2015;456(1):41-6. 

López-Vicario, Cristina; Alcaraz-Quiles, José; García-Alonso, Verónica; Rius, Bibiana; Hwang, Sung H; Titos, Esther; Lopategi, Aritz; Hammock, Bruce D; Arroyo, Vicente; Clària, Joan. Inhibition of soluble epoxide hydrolase modulates inflammation and autophagy in obese adipose tissue and liver: role for omega-3 epoxides. Proceedings Of The National Academy Of Sciences Of The United States Of America. 2015;112(2):536-41. 

Tamura, Yukinori; Kawao, Naoyuki; Yano, Masato; Okada, Kiyotaka; Okumoto, Katsumi; Chiba, Yasutaka; Matsuo, Osamu; Kaji, Hiroshi. Role of plasminogen activator inhibitor-1 in glucocorticoid-induced diabetes and osteopenia in mice. Diabetes. 2015;64(6):2194-206. 

Hiyoshi, Tetsuaki; Marumo, Toshiyuki; Hikichi, Hirohiko; Tomishima, Yasumitsu; Urabe, Hiroki; Tamita, Tomoko; Iida, Izumi; Yasuhara, Akito; Karasawa, Jun-ichi; Chaki, Shigeyuki. Neurophysiologic and antipsychotic profiles of TASP0433864, a novel positive allosteric modulator of metabotropic glutamate 2 receptor. The Journal Of Pharmacology And Experimental Therapeutics. 2014;351(3):642-53. 

Park, Jinyoung; Yoon, Young-Sil; Han, Hye-Sook; Kim, Yong-Hoon; Ogawa, Yoshihiro; Park, Keun-Gyu; Lee, Chul-Ho; Kim, Seong-Tae; Koo, Seung-Hoi. SIK2 is critical in the regulation of lipid homeostasis and adipogenesis in vivo. Diabetes. 2014;63(11):3659-73. 

Tamura, Isao; Ohkawa, Yasuyuki; Sato, Tetsuya; Suyama, Mikita; Jozaki, Kosuke; Okada, Maki; Lee, Lifa; Maekawa, Ryo; Asada, Hiromi; Sato, Shun; Yamagata, Yoshiaki; Tamura, Hiroshi; Sugino, Norihiro. Genome-wide analysis of histone modifications in human endometrial stromal cells. Molecular Endocrinology (Baltimore, Md.). 2014;28(10):1656-69. 

Watanabe, Akio; Kato, Tsuyoshi; Ito, Yusuke; Yoshida, Izumi; Harada, Teppei; Mishima, Takashi; Fujita, Kazuhiro; Watai, Masatoshi; Nakagawa, Kiyotaka; Miyazawa, Teruo. Aculeatin, a coumarin derived from Toddalia asiatica (L.) Lam., enhances differentiation and lipolysis of 3T3-L1 adipocytes. Biochemical And Biophysical Research Communications. 2014;453(4):787-92. 

Takahashi, H; Sato, K; Yamaguchi, T; Miyake, M; Watanabe, H; Nagasawa, Y; Kitagawa, E; Terada, S; Urakawa, M; Rose, M T; McMahon, C D; Watanabe, K; Ohwada, S; Gotoh, T; Aso, H. Myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double-muscled (DM) and normal-muscled (NM) Japanese shorthorn cattle. Domestic Animal Endocrinology. 2014;48( 24906930):62-8. 

Ohminami, Hirokazu; Amo, Kikuko; Taketani, Yutaka; Sato, Kazusa; Fukaya, Makiko; Uebanso, Takashi; Arai, Hidekazu; Koganei, Megumi; Sasaki, Hajime; Yamanaka-Okumura, Hisami; Yamamoto, Hironori; Takeda, Eiji. Dietary combination of sucrose and linoleic acid causes skeletal muscle metabolic abnormalities in Zucker fatty rats through specific modification of fatty acid composition. Journal Of Clinical Biochemistry And Nutrition. 2014;55(1):15-25. 

Hiyoshi, Tetsuaki; Marumo, Toshiyuki; Hikichi, Hirohiko; Tomishima, Yasumitsu; Urabe, Hiroki; Tamita, Tomoko; Iida, Izumi; Yasuhara, Akito; Karasawa, Jun-ichi; Chaki, Shigeyuki. Neurophysiologic and antipsychotic profiles of TASP0433864, a novel positive allosteric modulator of metabotropic glutamate 2 receptor. The Journal Of Pharmacology And Experimental Therapeutics. 2014;351(3):642-53. 

Salvador-Adriano, Ana; Vargas-Chávez, Sonia; Hernández-Vázquez, Alain de J; Ortega-Cuellar, Daniel; Tovar, Armando R; Velázquez-Arellano, Antonio. Insulin sensitivity is inversely related to cellular energy status, as revealed by biotin deprivation. American Journal Of Physiology. Endocrinology And Metabolism. 2014;306(12):E1442-8. 

Yamada, Hidetoshi; Oshiro, Eriko; Kikuchi, Sayaka; Hakozaki, Mayuka; Takahashi, Hideyuki; Kimura, Ken-Ichi. Hydroxyeicosapentaenoic acids from the Pacific krill show high ligand activities for PPARs. Journal Of Lipid Research. 2014;55(5):895-904. 

Shimizu, Ippei; Aprahamian, Tamar; Kikuchi, Ryosuke; Shimizu, Ayako; Papanicolaou, Kyriakos N; MacLauchlan, Susan; Maruyama, Sonomi; Walsh, Kenneth. Vascular rarefaction mediates whitening of brown fat in obesity. The Journal Of Clinical Investigation. 2014;124(5):2099-112. 

Morita, Sumiyo; Horii, Takuro; Kimura, Mika; Arai, Yuji; Kamei, Yasutomi; Ogawa, Yoshihiro; Hatada, Izuho. Paternal allele influences high fat diet-induced obesity. Plos One. 9(1):e85477. 

Baker, Nicki A; English, Victoria; Sunkara, Manjula; Morris, Andrew J; Pearson, Kevin J; Cassis, Lisa A. Resveratrol protects against polychlorinated biphenyl-mediated impairment of glucose homeostasis in adipocytes. The Journal Of Nutritional Biochemistry. 2013;24(12):2168-74. 

Woo, Mi-Seon; Choi, Hyeon-Son; Lee, Ok-Hwan; Lee, Boo-Yong. The edible red alga, Gracilaria verrucosa, inhibits lipid accumulation and ROS production, but improves glucose uptake in 3T3-L1 cells. Phytotherapy Research : Ptr. 2013;27(7):1102-5. 

Fujimori, Ko; Shibano, Makio. Avicularin, a plant flavonoid, suppresses lipid accumulation through repression of C/EBPα-activated GLUT4-mediated glucose uptake in 3T3-L1 cells. Journal Of Agricultural And Food Chemistry. 2013;61(21):5139-47. 

DesRochers, Teresa M; Suter, Laura; Roth, Adrian; Kaplan, David L. Bioengineered 3D human kidney tissue, a platform for the determination of nephrotoxicity. Plos One. 8(3):e59219. 

Monden, Masayo; Koyama, Hidenori; Otsuka, Yoshiko; Morioka, Tomoaki; Mori, Katsuhito; Shoji, Takuhito; Mima, Yohei; Motoyama, Koka; Fukumoto, Shinya; Shioi, Atsushi; Emoto, Masanori; Yamamoto, Yasuhiko; Yamamoto, Hiroshi; Nishizawa, Yoshiki; Kurajoh, Masafumi; Yamamoto, Tetsuya; Inaba, Masaaki. Receptor for advanced glycation end products regulates adipocyte hypertrophy and insulin sensitivity in mice: involvement of Toll-like receptor 2. Diabetes. 2013;62(2):478-89. 

Ishikawa-Kobayashi, Eiko; Ushijima, Kentarou; Ando, Hitoshi; Maekawa, Tomohiro; Takuma, Masashi; Furukawa, Yusuke; Fujimura, Akio. Reduced histone H3K9 acetylation of clock genes and abnormal glucose metabolism in ob/ob mice. Chronobiology International. 2012;29(8):982-93. 

Gherardi, Gaia; Nogara, Leonardo; Ciciliot, Stefano; Fadini, Gian Paolo; Blaauw, Bert; Braghetta, Paola; Bonaldo, Paolo; De Stefani, Diego; Rizzuto, Rosario; Mammucari, Cristina. Loss of mitochondrial calcium uniporter rewires skeletal muscle metabolism and substrate preference. Cell Death And Differentiation. 2019;26(2):362-381.