Staining calcified bone nodules with alizarin red S

Calcification staining kit

The calcification staining kit is a staining kit that contains alizarin red S, a dye that binds to calcium, and is designed to easily stain calcified bone nodules.

Intended use

Osteoblasts, which are responsible for bone formation, are known to increase alkaline phosphatase, osteocalcin, etc. over time depending on the degree of differentiation and form bone nodules with deposited calcium.

This kit is a staining kit containing alizarin red S, a dye that binds to calcium, and is designed to easily stain calcified bone nodules.

Composition

• Buffer (100 times concentrated) 60 mL 1 bottle
• 10 tubes for 10 mL staining substrate

* Ten 24-well plates can be stained.
* AK21 does not contain fixative (methanol). Please purchase separately.

Examples of calcification staining

Staining calcified 3T3-E1 cells

Staining calcified rat osteoblasts (cultured in 12-well plate)

Staining of mineralized osteogenic culture kit cells

 

Catalog Number	Product Name	Size
PMC-AK21	Calcified Nodule Staining kit	1 Set