Alkaline Phosphatase Staining Kit

Cosmo Bio LTD

Catalog No: PMC-AK20

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Alkaline Phosphatase Staining Kit is designed for the rapid and sensitive detection of osteoblasts differentiated in culture from bone marrow cells. Bone forming osteoblasts and bone-degrading osteoclasts can be distinguished by the expression, respectively, of alkaline phosphatase and tartrate-resistant acid phosphatase (TRAP). CosmoBio’s TRAP staining kit (PMC-AK04F-COS) is ideal for rapid and convenient detection of the latter.

Simple staining protocol

1. Remove culture medium. Wash each well 3 times with 1mL PBS.
2. Add 500μL of 10% formalin neutral buffer solution to each well and fix for 20 minutes at room temperature.
3. Remove 10% formalin neutral buffer solution and wash each well 3 times with 2mL deionized water.
4. Dissolve 1 vial of Chromogenic Substrate in 5mL Substrate-containing Buffer.
5. Add 400μL Chromogenic Substrate to each well.
6. Incubate at 37°C for 5-20 minutes. Adjust incubation time until stained.
7. Wash with deionized water to stop the reaction.

References

1. A. S. H. De Jong, M. Van Kessel-Van Vark and A. K. Raap. Sensitivity of various visualization methods for peroxidase and alkaline phosphatase activity in immunoenzyme histochemistry. The Histochemical Journal Volume 17, 1119-1130 (1985)

Citations for Alkaline Phosphatase Staining Kit – 4 Found

Azuma, Kotaro et al. 2024. EBAG9-deficient mice display decreased bone mineral density with suppressed autophagy. https://www.cell.com/iscience/home. 27(2):108871.   , Journal
- Comments: Cosmo Bio’s Alkaline Phosphatase (ALP) Staining Kit was used to detect osteoblastic activity in a study showing Ebag9-knockout mice exhibit fragile bones with low bone mineral density due to decreased bone formation and enhanced bone resorption arising from a role with interacting protein TMSF1 in osteoblast and osteoclast autophagy.

Ali et al. 2019. Lithium-containing surface pre-reacted glass fillers enhance hDPSC functions and induce reparative dentin formation in a rat pulp capping model through activation of Wnt/β-catenin signaling. Acta Biomaterialia. :.   , Journal

Linh et al. 2016. Augmenting in vitro osteogenesis of a glycine-arginine-glycine-aspartic-conjugated oxidized alginate-gelatin-biphasic calcium phosphate hydrogel composite and in vivo bone biogenesis through stem cell delivery. J Biomater Appl. 31(5):661-73.   , Journal

et al. .

Terao, Y., Sasagawa, K., Domon, H., Hirayama, S., Maekawa, T., Isono, T., and Tabeta, K. (2024) Secretory leucocyte protease inhibitor regulates bone metabolism and inflammation in experimental mouse periodontitis. 10.21203/rs.3.rs-3806269/v1.

Cosmo Bio's Alkaline Phosphatase (ALP) Staining Kit was used to detect osteoblastic activity in a study suggesting that secretory leukocyte protease inhibitor (SLPI) prevents periodontal tissue destruction by affecting inflammation and bone metabolism. showing that intragingival administration of SLPI significantly reduced neutrophil elastase activity in periodontal tissue and alleviated alveolar bone loss in mice.

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