The Sievewell Slide: A new microfluidic device for ultra-high density single cell capture, culture, processing and imaging

Tokyo Ohka Kogyo

Catalog No.: TOK-SWS-2001-5

Background

Arraying cells is an important step for single cell analysis. For example, cell overlap is unfavourable for single cell imaging and isolation. Microcavity arrays are used to capture single cells. In general, the distribution of cells in a cavity is by sedimentation and the single cell rate follows a Poisson distribution. Its single cell capture rate is relatively low, especially when loading cells in higher quantities.

Product Information

• SIEVEWELL™ Slide Dimensions (W x D x H): 25 mm x 75 mm x 12 mm
• Microscope compatible
• Chamber area: 17 mm x 17 mm
• Working volume: 0.5 mL to 2 mL
• Material: biocompatible polymer, PS, PC Sterilized by gamma radiation
• 5 pcs per package

---

Frequently Asked Questions

Is it possible to culture cells in SIEVEWELL Slide?
Yes. SIEVEWELL Slide includes lid for cell culture.

Do cells grow directly on nanowell surface?
Yes. However, some cells attach to nanowell and some cells do not attach but grow in suspended state. Please test culture with your cell of interest with a free sample.

Is it possible to image live cell with SIEVEWELL Slide?
Please find the example of application here.

Is it possible to retrieve cells from SIEVEWELL Slide?
Yes. Cell retrieval is by several pushing back from the side port.

Is SIEVEWELL Slide compatible with every type of microscope?
SIEVEWELL Slide is compatible with any inverted microscope that has a regular stage or a frame.

Is high-resolution microscopy (40x, 60x, 100x) possible with SIEVEWELL Slide?
High-resolution microscopy is impossible due to the thickness of the bottom plate. However, we will propose the solution for that. Please contact us.

Which solvents and chemicals are compatible with SIEVEWELL Slide?
Acetone: no
Some of the compatible solvents may turn out to be incompatible under certain physical conditions such as temperature, strain, or when used over an extensive period of time. Please test the chemical compatibility of your compound with a free sample. What is your recommendation for reusing SEIVEWELL Slide? We do not recommend reusing the slides.

Citations for The Sievewell Slide: A new microfluidic device for ultra-high density single cell capture, culture, processing and imaging – 5 Found

Yang, Liwen; Rivandi, Mahdi; Franken, André; Hieltjes, Maarten; van der Zaag, Pieter Jan; Nelep, Constantin; Eberhardt, Jens; Peter, Stefan; Niederacher, Dieter; Fehm, Tanja; Neubauer, Hans. Implementing microwell slides for detection and isolation of single circulating tumor cells from complex cell suspensions. Cytometry. Part A : The Journal Of The International Society For Analytical Cytology. 2022;101(12):1057-1067. 

Li, Zhenlong; Ma, Rui; Tang, Hejun; Guo, Jiamin; Shah, Zahir; Zhang, Jianying; Liu, Ningyuan; Cao, Shuai; Marcucci, Guido; Artis, David; Caligiuri, Michael A; Yu, Jianhua. Therapeutic application of human type 2 innate lymphoid cells via induction of granzyme B-mediated tumor cell death. Cell. 2024;187(3):624-641.e23. 

Vu, Anh Hai; Kang, Moonyoung; Wurlitzer, Jens; Heinicke, Sarah; Li, Chenxin; Wood, Joshua C; Grabe, Veit; Buell, C Robin; Caputi, Lorenzo; O'Connor, Sarah E. Quantitative Single-Cell Mass Spectrometry Provides a Highly Resolved Analysis of Natural Product Biosynthesis Partitioning in Plants. Journal Of The American Chemical Society. 2024;146(34):23891-23900. 

Li, Chenxin; Wood, Joshua C; Vu, Anh Hai; Hamilton, John P; Rodriguez Lopez, Carlos Eduardo; Payne, Richard M E; Serna Guerrero, Delia Ayled; Gase, Klaus; Yamamoto, Kotaro; Vaillancourt, Brieanne; Caputi, Lorenzo; O'Connor, Sarah E; Robin Buell, C. Single-cell multi-omics in the medicinal plant Catharanthus roseus. Nature Chemical Biology. 2023;19(8):1031-1041. 

Vu, Anh Hai; Kang, Moonyoung; Wurlitzer, Jens; Heinicke, Sarah; Li, Chenxin; Wood, Joshua C; Grabe, Veit; Buell, C Robin; Caputi, Lorenzo; O'Connor, Sarah E. Quantitative Single-Cell Mass Spectrometry Provides a Highly Resolved Analysis of Natural Product Biosynthesis Partitioning in Plants. Journal Of The American Chemical Society. 2024;146(34):23891-23900.