HA tagged Protein PURIFICATION KIT (Trial Kit)

MBL Life Sciences

Product Description

The HA epitope tag, YPYDVPDYA, is derived from Human influenza hemaglutinin (HA) 98-106 aa. This HA tag is also commonly used in such as mammalian cell expression vectors. MBL's HA-tagged Protein PURIFICATION KIT is designed for the isolation of HA-tagged protein from cell culture supernatants containing serum and cell lysate under neutral pH condition. Severe conditions such as acidic or alkaline elution denature protein structure. However, a neutral pH elution can preserve protein activity and native conformation. MBL has developed the Anti-HA tag Beads to purify HA-tagged proteins quickly and efficiently. As the Beads can be used at neutral pH, the purified proteins can maintain the activity and conformation. The elution of HA-tagged proteins from the Beads is achieved by the addition of the HA peptide. As the HA peptide competes with HA-tagged proteins on the Beads, the purified proteins do not lose the protein activity. Using a Spin Column resulting in high efficiency has optimized the simple procedure of this kit.

Quick and efficient purification of HA-tagged protein at a neutral pH to maintain the isolated protein's activities and native conformations.

References

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