Anti CD9 Antigen (MRP-1/Tspan-29) mAb (Clone 12A12)

Cosmo Bio

SKU:
CAC-SHI-EXO-M01-50
  • Figure 4. ELISA of C32 melanoma cell exosomes with anti-CD9 (12A12-ALP) antibody (5 ng/mL).
  • Figure 6. Comparison of exosome antibodies. (Left) Detection of exosomes with CD9 antibody (clone 12A12) and comparative clone A. Clone A failed to generate a signal while 12A12 detected a strong signal. (Right) Detection of exosomes with CD63 antibody (clone 8A12) and comparative clone A. Clone A detected a weak signal while 8A12 detected a strong signal.
  • Figure 5. CD9 and CD63 molecules on the surface of extracellular vesicles from human breast cancer cell line (MDA-MB-231-luc-D3H2LN)) were detected by anti-human CD9 antibody (SHI-EXO-M01) and anti-human CD63 antibody (SHI-EXO-M02).
  • Figure 1. Generic exosome shown with commonly associated molecules. Not all molecules will be found with any given exosome and other molecules may also be found.
  • Figure 2. Western blot analysis of CD9 in C32 melanoma cell exosomes using anti-CD9 (12A12) antibody. Sample: C32 cell Exosome (5ug, 1ug) Primary antibody: anti-CD9 (12A12) (1ug/mL) Secondary antibody: anti-mouse IgG-HRP
  • Figure 3. IP-WB of human serum exosomes. Sample 1. Anti-CD9 (12A12) - Serum A 2. Control antibody - Serum A 3. Anti-CD9 (12A12) - Serum B 4. Control antibody - Serum B 5. Ultracentrifugation - Serum A 6. Ultracentrifugation - Serum A
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CD9 is a cell surface glycoprotein which belongs to the tetraspanin superfamily. CD9 is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. Importantly, it is found on the surface of exosomes.

Exosomes are cell-derived vesicles bounded by a lipid bilayer membrane and exhibiting a diameter of 50 to 150 nm. They are secreted from cultured cells and are observed in body fluids such as saliva, blood, urine, amniotic fluid, malignant ascites. Recent studies indicate that exosomes contain various proteins and RNAs, suggesting a role in information transfer between cells.

This monoclonal antibody can be used to immunoprecipitate exosomes from serum and culture supernatants.

References:
1) Shigeyasu Tsuda et al., Scientific Reports volume 7, Article number: 12989 (2017)
2) N Nishida-Aoki et al., Mol Ther. 2017 Jan 4;25(1):181-191. doi: 10.1016/j.ymthe.2016.10.009.
3) Matsuzaki K et al., Oncotarget. 2017 Apr 11; 8(15): 24668–24678. doi: 10.18632/oncotarget.14969
4) Kazutoshi Fujita et al., Sci Rep. 2017; 7: 42961. doi: 10.1038/srep42961
5) Yoshioka Y et al., Nat Commun. 2014 Apr 7;5:3591. doi: 10.1038/ncomms4591.
6) Saito S et al., Sci Rep. 2018 Mar 5;8(1):3997. doi: 10.1038/s41598-018-22450-2.
7) Yagi Y et al., Neurosci Lett. 2017 Jan 1;636:48-57. doi: 10.1016/j.neulet.2016.10.042.
8) Ueda K et al., Sci Rep. 2014 Aug 29;4:6232. doi: 10.1038/srep06232.



Product Specifications
Application IP, ELISA, WB, FC, IEM
Reactivity Human
Clonality Monoclonal (Clone No.: 12A12)
Host Mouse


Documents & Links for Anti CD9 Antigen (MRP-1/Tspan-29) mAb (Clone 12A12)
Datasheet cac-shi-exo-m01-50_anti-cd9-mab-clone-12a12_datasheet.pdf

Documents & Links for Anti CD9 Antigen (MRP-1/Tspan-29) mAb (Clone 12A12)
Datasheet cac-shi-exo-m01-50_anti-cd9-mab-clone-12a12_datasheet.pdf



Citations for Anti CD9 Antigen (MRP-1/Tspan-29) mAb (Clone 12A12) – 4 Found
Jingushi, Kentaro; Uemura, Motohide; Nakano, Kosuke; Hayashi, Yujiro; Wang, Cong; Ishizuya, Yu; Yamamoto, Yoshiyuki; Hayashi, Takuji; Kinouchi, Toshiro; Matsuzaki, Kyosuke; Kato, Taigo; Kawashima, Atsunari; Ujike, Takeshi; Nagahara, Akira; Fujita, Kazutoshi; Ueda, Koji; Tsujikawa, Kazutake; Nonomura, Norio. Leukocyte‑associated immunoglobulin‑like receptor 1 promotes tumorigenesis in RCC. Oncology Reports. 2019;41(2):1293-1303.  PubMed
Torii, Yuka; Suzuki, Takako; Fukuda, Yuto; Haruta, Kazunori; Yamaguchi, Makoto; Horiba, Kazuhiro; Kawada, Jun-Ichi; Ito, Yoshinori. MicroRNA expression profiling of urine exosomes in children with congenital cytomegalovirus infection. Scientific Reports. 2024;14(1):5475.  PubMed
Masaki, Kanako; Ahmed, Abo Bakr F; Ishida, Takenori; Mikami, Yuuki; Funabashi, Hisakage; Hirota, Ryuichi; Ikeda, Takeshi; Kuroda, Akio. Chromatographic purification of small extracellular vesicles using an affinity column for phospholipid membranes. Biotechnology Letters. 2023;45(11-12):1457-1466.  PubMed
Yamada, Norihiro; Tominaga, Kana; Tominaga, Naoomi; Kobayashi, Ayumi; Niino, Chihiro; Miyagi, Yuta; Yamagata, Hirotaka; Nakagawa, Shin. Glycosylation changes of vWF in circulating extracellular vesicles to predict depression. Scientific Reports. 2024;14(1):29066.  PubMed
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