Brochure Download
(8 page pdf)
Supplier's product pages
(sievewell.com)
User Guide
(4 pages pdf)
HIGH DENSITY SINGLE CELL ARRAY DEVICE
Not your typical 370,000 and 90,000 microwell plates!
SIEVEWELL™ is a new, innovative microfluidics platform for single cell capture, culture, imaging and analysis.
Ordering Information
| Product name (click for order info) | Cat no (click for user guide) | Size | |
| The SIEVEWELL™ Slide: A new microfluidic device for ultra-high density single cell capture, culture, processing and imaging |
370.000 wells per slide, well size = 20 microns across each (5 pack) 90,000 wells per slide, well size = 50 microns across |
||
About SIEVEWELL™
At the heart of Sievewell is an ultra-high density, optically transparent membrane array of cell sized, cell culture-friendly wells precision manufactured by photolithography. Each well contains 2 pores through which fluid can pass. Due to SIEVEWELL's unique design, suction applied through Sievewell’s fluid aspiration ports (with a hand pipette) creates a downward flow. This innovative design enables:
- Single cell per well capture rates significantly higher than by gravity alone
- At-will fluid exchange with downward flow working to keep cells in place. Ideal for cell washing, staining, and other treatment to cells.
- Simple optical observation, imaging and evaluation.
For much more product and application information, please download the Sievewell 8-page Product Brochure (sidebar this page), browse the image gallery below, and visit SIEVEWELL.COM.
The following list indexes the page content at SIEVEWELL.COM, the manufacturer's site (external links).
• Introductory image slider with links to other pages and videos on SIEVWELL.COM.
• Don't Miss Your Rare Cells (with introductory video); Arraying Cells at High Density; On-chip Staining Without Cell Loss; No Pump Needed. Just Pipette; Single Cell Culture.
• Product Specifications; 8-page brochure download; SIEVEWELL FAQ
• Introductory Video (same as on Home page)
• Membrane Film With Microwells; No Pump Required. Just Pipette; On-chip Staining Without Cell Loss; Single cell culture
Applications
• Single cell culture
time lapse of K562 cells cultured on 50 micron Sievewell slide.
• Cell-Cell Interaction Assays
Monitor NK cell cytotoxicity
• Calcium Flux Assay in Suspension Cells
SIEVEWELL™ vs standard microplate using Fluo4-AM treated K562 cells followed by ionomycin addition.
• Culture of Cardiomyocytes and Calcium Imaging
Cell culture and imaging of IPS cell-derived cardiomyocytes expressing a calcium sensor. Video shows culture of cardiomyocytes and calcium cycling.
• On-chip Neural differentiation of PC12 cells
Rat pheochromocytoma PC12 cells stained with anti-tubulin ß3 and DAPI after culture with NGF
• Detection of circulating tumor cells from CDX/PDX-tumor bearing mouse
Blood cells from mouse xenografted with fluorescent-labeled tumor loaded on Sievewell and lysed.
• Detection of Neutrophil extracellular traps (NETs)
Stimulation of human PBMC by PMA and staining and visualization of NETs
• Real-time imaging to quantify NETosis with SIEVEWELL
• Mouse blood separation
Separation of lymphocytes from small volume of mouse blood
• Tissue dissociation on chip
Direct dissociation of mouse tissue to obtain rare cell population
• On chip CTC staining, detection
Staining and detection of human PBMC spiked with MCF
• Imaging with Confocal Microscopy
Library
One-page flyer
References Page
Procedures Page:
• General Use Notes Videos (focus on pipette use for fluid control)
• Step 1 Videos - priming the slide
• Step 2 Videos - procedure for cell loading
• Step 3 Videos - procedure for cell staining
SIEVEWELL™ Image Gallery
(right click and select open in new window to view full size)
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
 |
 |