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Staining osteoclast marker 'tartrate-resistant acid phosphatase'

TRAP staining kit

The TRAP staining kit can be used to stain the osteoclast marker "Tartrate-Resistant Acid Phosphatase (TRAP)".

Background

Bone mass is controlled by the balance between the activity of bone-forming osteoblasts and the activity of bone-destroying osteoclasts. Osteoblasts have alkaline phosphatase as a marker, whereas osteoclasts have a marker of tartrate-resistant acid phosphatase.

Intended use

Composition

  • Buffer containing 50 mM tartaric acid (pH 5.0) 50 ml 1 bottle
  • Chromogenic substrate 3mg 10 bottles

* Does not include fixative (10% neutral buffered formalin). Please purchase separately or make your own according to the attached manual.

Dyeing example

PMC_00480003_B-50-200-10K_1.jpg

Figure 1: Examples of TRAP staining

TRAP images

Figure 2: Staining Rat Osteoclasts

Frequently asked questions and answers (FAQ)

Q: Is it possible to quantify after staining?

A: Quantitation is not possible due to the lack of suitable solvents for the extraction of colored pigments.

 

References

  1. Morinobu, A., Biao, W., Tanaka, S., Horiuchi, M., Jun, L., Tsuji,G., Sakai, Y., Kurosaka, M., Kumagai, S.
    (-)-Epigallocatechin-3-Gallate Suppresses Osteoclast Differentiation and Ameliorates Experimental Arthritis in Mice.
    Arthritis Rheum. 58, 2012-2018 (2008), (PMID:18576345)
  2. Hase, H., Kanno, Y., Kojima, H., Sakurai, D., Kobata, T.
    Coculture of Osteoclast Precursors With Rheumatoid Synovial Fibroblasts Induces Osteoclastogenesis via Transforming Growth Factor beta-Mediated Down-Regulation of Osteoprotegerin.
    Arthritis Rheum. 58, 3356-3365 (2008), (PMID:18975335)
  3. Cao, H., Kuboyama, N.
    A Biodegradable Porous Composite Scaffold of PGA/beta-TCP for Bone Tissue Engineering.
    Bone. 46, 386-395 (2010), (PMID:19800045)
  4. Okada, Y., Hamada, N., Kim, Y., Takahashi, Y., Sasaguri, K., Ozono, S., Sato, S.
    Blockade of Sympathetic beta-receptors Inhibits Porphyromonas Gingivalis-induced Alveolar Bone Loss in an Experimental Rat Periodontitis Model.
    Arch. Oral Biol. 55, 502-508 (2010), (PMID:20593554)
  5. Okamoto, A., Ohnishi, T., Bandow, K., Kakimoto, K., Chiba, N., Maeda, A., Fukunaga, T., Miyawaki, S., Matsuguchi, T.
    Reduction of Orthodontic Tooth Movement by Experimentally Induced Periodontal Inflammation in Mice.
    Eur. J. Oral Sci. 117, 238-247 (2009), (PMID:19583750)
  6. Fujiwara, T., Fukushi, J., Yamamoto, S., Matsumoto, Y., Setsu, N., Oda, Y., Yamada, H., Okada, S., Watari, K., Ono, M., Kuwano, M., Kamura, S., Iida, K., Okada, Y., Koga, M., Iwamoto, Y.
    Macrophage Infiltration Predicts a Poor Prognosis for Human Ewing Sarcoma.
    Am. J. Pathol. 179, 1157-1170 (2011), (PMID:21771572)
Catalog Number Product Name Size
PMC-AK04F-COS TRAP Staining Kit 10 Plates