Polyubiquitylated conjugates, mAb (FK1)

Ubiquigent

Catalog No: UBI-68-0111-500

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Application: WB 
Clonality: Monoclonal 
Host: Mouse 
Reactivity: Human 

Source: BALB/C Mouse implantation ascites  
Formula: 10mM phosphate buffer, 0.15M NaCl PH7.4, 0.1% sodium azide  
Concentration: 1 mg/ml  
Molecular Weight: 150kDa  
Protein Sequence: n/a  
Quality Control Protein Identification: n/a  
Quality Control Activity: Anti-Polyubiquitylated Conjugates mAb (FK1) Antibody Activity Assay: The activity of anti-polyubiquitylated conjugates mAb (FK1) antibody was tested for its ability to recognise polyubiquitin chains (Lane 3) but not mono-ubiquitin conjugates or free ubiquitin. By Western blotting the specific recognition of polyubiquitin conjugates by the anti-polyubiquitylated conjugates mAb (FK1) over mono-ubiquitin conjugates (Di-ubiquitin) or free ubiquitin was demonstrated. In a priming and extension assay containing, UBE1 [6His-tagged] (Cat# 61-0001), UBE2W [6His-tagged] (Cat# 62-0085), UBE2N [untagged] (Cat# 62-0047), UBE2V1 [untagged] (Cat# 62-0059), Ubiquitin (Cat# 60-0001), CHIP [untagged] (Cat# 63-0003) and ATP. Using the antipolyubiquitylated conjugates mAb (FK1) antibody, detection of polyubiquitin chains extending from mono-ubiquitylated CHIP and free chains generated by UBE2N/UBE2V1 in the presence of CHIP were observed.