N-Histofine ALK Control Slides

Cosmo Bio LTD

Catalog No: NIC-417081F

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N-Histofine® ALK Control Slides (from Nichirei Biosciences) comprises 5 slides with formalin-fixed paraffin-embedded (FFPE) cell lines [NCI-H2228 (ALK positive) and SK-BR-3 (ALK negative)] mounted on each slide for use as standards for evaluating ALK protein expression in human tissue sections. ALK Control Slides are for validation of reagent performance and IHC (and ICC) staining technique when using N-Histofine® ALK Detection Kit. For more information please explore below and visit our Nichirei Biosciences Dashboard.



Background



The ALK gene located at 2p23 encodes a receptor-type tyrosine kinase belonging to the insulin receptor family. In 1994 the t(2;5)(p23;q35) translocation in anaplastic large-cell lymphoma (ALCL) was shown to result in a fusion between the ALK and NPM (Nucleophosmin) genes. A kinase domain in the ALK protein intracellular region is associated with cell growth promotion and apoptosis inhibition. The proteins produced from ALK fusion genes are chronically activated by dimerization, leading to the development of cancer. Subsequent work has shown that the ALK gene forms fusions with a large number of genes. These include for ALCL: ATIC, CLTC, MSN, TPM3, TPM4, TFG, MYH9 and ALO17; and for inflammatory myofibroblastic tumors (IMT): ATIC, CARS, CLTC, DCTN1, TPM3, TPM4, PPFIBP1, RANBP2 and SEC31L1. Recently, additional ALK fusion genes have been reported, including with EML4, KIF5B and KCL1 in non-small cell lung cancer (NSCLC); SEC31A and SQSTM1 in ALK-positive large B-cell lymphoma; and VCL in renal cell cancer.



INTENDED FOR RESEARCH USE ONLY