Insulin ELISA

Cosmo Bio LTD

Catalog No: YII-YK060-EX

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Reactivity: Domesticated Animal, Mammalian - Other, Rabbit, Human 
This ELISA kit is used for quantitative determination of insulin in serum sample of human, rabbit and dog. The kit is characterized for sensitive quantification, high specificity and no influences with other components in samples and needlessness of sample pretreatment. Human insulin standard is recombinant product.Supplementary: Sandwich

Applicable Sample: Serum

Other: This kit is a stable and convenient assay system for measurement of insulin (human, rabbit and dog) in serum samples. The processing of proinsulin, which occurs within the B cell,yieldsinsulin and C-peptide. Insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of insulin in blood is very important, and also provides valuable information to evaluate the pancreatic B cell function.

This kit for determination of insulin concentration (IR-insulin) in serum samples of human, rabbit and dog is based on a sandwich enzyme immunoassay by using combination of guinea pig anti human insulin antibody (coated on plate), recombinant insulin standard, biotinylated guinea pig anti human insulin antibody and HRP labeled streptoavidin (SA-HRP). Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of insulin is calculated.





Citations for Insulin ELISA – 1 Found

Ash et al. 2011. The molecular basis by which dietary restricted feeding reduces mitochondrial reactive oxygen species generation. Mech Ageing Dev. 132(1-2):43-54.   , Journal