Anti Holliday Junction ATP-Dependent DNA Helicase (RuvB) pAb (Mouse, Antiserum)

Cosmo Bio LTD

Catalog No: BAM-61-007-EX

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Application: WB 
Clonality: Polyclonal 
Host: Mouse 
Purification: Serum 
Reactivity: E. coli 
E. coli RuvA protein binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair, and forms a complex with RuvB motor protein, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above, holding it in between (1, 2). Using this antiserum in Western blotting, the band of 22kD corresponding to RuvA was obtained from the extract of E. coli cells (Fig.1).