Anti Kinetochore-Associated Protein DSN1 Homolog (MIS13) pAb (Rabbit, Antiserum)

Cosmo Bio LTD

Catalog No: BAM-70-101-EX

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Application: IF, WB, ICC 
Clonality: Polyclonal 
Host: Rabbit 
Purification: Serum 
Reactivity: Human, Mouse, Rat 

 

CARD HyperOva - the super superovulation reagent

 

 

CARD HyperOva® (Japanese Patent No. 5927588) and CARD HyperOva® FD are superovulation reagents that induce ovulation at higher efficiency than conventional methods (PMSG-hCG) yielding 3-4 times more oocytes on average with C57BL/6J mice. They are also effective with other strains. Moreover, further gains in efficiency can be realized by using CARD Medium during the IVF procedure.

Why Use CARD HyperOva®?

Reduce Animal Use (contribution to the 3Rs of animal welfare - "Replacement, Reduction, Refinement"
Using CARD HyperOva® can reduce the number of oocyte donor mice by a factor of 1/3-1/4.

Optimization of Genetically Modified mouse Creation and Preservation
More ova from less mice usin CARD HyperOva® facilitates in vitro fertilization and embryo transfer.

Application to Valuable Genetically modified Mice
More ova from less mice using CARD HyperOva® on your valuable modified strains enables the large-scale preparation of embryos.
 

 

 

 

 

Composition

Anti-inhibin antibody.
Purified equine villus chorionic gonadotropin (eCG).

 

HyperOva Performance in Various Mouse Strains
* 7.5 IU of CARD HyperOva® or PMSG was injected to four weeks old female mice, then 7.5 IU of hCG was administered 48 hours later. Thereafter all ova obtained 17 hours after hCG injection were collected. * The in vitro fertilization rate is defined as the number of 2-cell embryos divided by the total number of ova (mean±SD). * Data cited from a paper published by Prof. Naoki Nakagata and Toru Takeo (Center for Animal Resources and development, Kumamoto University)
No.	Strain	Injection	Number of Oocyte Donors	Number of Inseminated Oocytes	Average Number of Ovulated Oocytes/Female	No. of Fertilized eggs	Fertilization Rate (%)
1	C57BL/6J	eCG	10	277	27.7±5.4	267	96.4±3.2
		HyperOva	10	1072	107.2±22.7	963	89.8±3.7
2	BALB/c ByJ	eCG	10	293	29.3±8.9	213	73.4±11.3
		HyperOva	10	903	90.3±14.5	680	76.4±9.0
3	C3H/HeJ	eCG	10	286	28.6±8.4	251	87.8±22.6
		HyperOva	10	520	52.0±18.1	446	85.8±15.5
4	DBA/2J	eCG	10	225	22.5±7.8	194	86.2±11.0
		HyperOva	10	688	68.8±13.6	592	86.0±4.5
5	FVB/NJ	eCG	10	168	16.8±3.3	159	94.6±6.7
		HyperOva	10	256	25.6±5.9	239	93.3±6.6
6	CD1	eCG	10	202	20.2±8.0	146	72.3±16.0
		HyperOva	10	337	33.7±9.9	269	79.8±19.6

 

HyperOva Frequently Asked Questions

Q: Is CARD Hyperova a mixture of eCG and inhibin antiserum?
A: Yes, it is mixed.

Q: Can you confirm the source of the horse gonadotropin…recombinant or purified? If recombinant, from what organism? If purified, what is the country of horse origin?
A: The horse gonadotropin of HyperOva is purified, and the origin country of horse is Japan.

Q: What is the country of goat origin?
A: The goat is a male Saanen goat who was born and raised in Japan.

Q: Did the goat co-mingle with any other livestock?
A: No. Only with other goats.

Q: Is the antibody unprocessed goat serum?
A: The goat serum was 2 times diluted by physiological saline. The horse gonadotropin was dissolved in saline, too.

Q: For the goat anti-mouse inhibin antiserum, what is the source of the immunogen?
A: It is a synthetic peptide.

Q: I can see a clump/precipitate/sediment in the vial.
A: The clumps doesn't affect the effectiveness of CARD HyperOva. Please avoid the clumps and aspirate only other solution.

Q: Can the product in 1ml vials be refrozen once thawed if we only need to use 0.3mls from a vial, at a time?”
A: Once the 1ml vial is opened, please use it to administrate intraperitoneally all at once. The 0.1mL - 0.2 mL can be administered in mice.

Q: Is the product working after it was stored at 4°C for one week instead of -20°C ?
A: We cannot guarantee the quality under this storage condition. Since the HyperOva may have been inactivated, we cannot recommend you to use it.

 

Citation
Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice.
Nakagawa Y, Sakuma T, Nishimichi N, Yokosaki Y, Takeo T, Nakagata N, Yamamoto T.
Biol Open. 2017 May 15;6(5):706-713. PMID: 28396487
Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing SgRNA/Cas9 Expressing Plasmids.
Noda T, Oji A, Ikawa M
Methods Mol Biol. 2017;1630:67-80. PMID: 28643250
Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice.
Nakagawa Y, Sakuma T, Nishimichi N, Yokosaki Y, Yanaka N, Takeo T, Nakagata N, Yamamoto T.
Biol Open. 2016 Aug 15;5(8):1142-8. PMID: 27387532
References
Immunotherapy using inhibin antiserum enhanced the efficacy of equine chorionic gonadotropin on superovulation in major inbred and outbred mice strains.
Takeo T, Nakagata N.
Theriogenology. 2016 Sep 15;86(5):1341-6. PMID: 27242176
Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice.
Takeo T, Nakagata N.
PLoS One. 2015 May 29;10(5):e0128330. PMID: 26024317