Anti DNA Polymerase 1 (POL 1) pAb (Rabbit, Antiserum)

Cosmo Bio LTD

Catalog No: BAM-61-012EX

Please click here to view other Bio Academia (BAM) Products

Application: IP, WB 
Clonality: Polyclonal 
Host: Rabbit 
Purification: Serum 
Reactivity: E. coli 
Application: 1. Western blot. (1/1,000~ 1/5,000 dilution)

2. Immunoprecipitation (assay dependent)

Not tested for other applications

Cross reactivity: E. coli DNA polymerase 1. Not tested in other species

Background

E. coli DNA polymerase 1 (928 aa; 103 kDa) is encoded by polA gene and involved in
DNA replication and repair. In addition to polymerase activity, this DNA polymerase exhibits 3'
to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a
template and can unwind the parental DNA strand from its template.

Application

Western blot. (1/1,000~ 1/5,000 dilution)
Immunoprecipitation (assay dependent)
Not tested for other applications

Fig.1 Western blot analysis of DNA polymerase 1 in
crude extract of E. coli cells by using anti-DNA
polymerase 1 antibody

1; Purified E. coli DNA polymerase 1 (10 ng)
2; Sonic lysate supernatant of
E. coli strain AB1157 cells.
Primary antibody was used at 1/2,000 dilution.

Predicted molecular mass of DNA polymerase 1 is 103 kDa.

Fig.2 Immunoprecipitation of DNA polymerase 1 from
crude lysate of E. coli with anti-DNA polymerase 1
antibody

1. Purified DNA polymerase 1 ( 20 ng), WB

2. Purified DNA polymerase 1 (10 ng), WB

3. Crude lysate of E. coli strain AB1157 (10 μg), WB

4. The crude lysate of E.coli cells was reacted with anti-DNA polymerase 1 antibody and
precipitated with protein G conjugated magnetic beads, and analyzed by WB by
using anti-DNA polymerase 1 antibody. As the secondary antibody, anti-rabbit IgG
antibody conjugated with HRP for IP (Abcam 131368) was used.