Anti Denticleless Protein Homolog (DTL/CDT2) pAb (Rabbit, Antiserum)

Cosmo Bio LTD

Catalog No: BAM-70-115-EX

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Application: IP, IF, WB, FC, ICC 
Clonality: Polyclonal 
Host: Rabbit 
Purification: Serum 
Reactivity: Mouse, Human, Hamster, Rat 
Western blotting: 1/2,000 dilution

Immunoprecipitation (1/100-1/200 dilution)

Immunofluorescence staining (1/100-1/1,000)

FACS (assay dependent)

Other applications have not been tested.

Optimal dilutions/concentrations should be determined by the e CDT2 (human, 730 aa, 79.5 kDa) is substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, alsonamed CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containingthe 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis. CDT2 is activated by checkpoint kinase ATR following DNA damage.

Cross reactivity: Human and mouse Cdt2 proteins. Not tested in other species