Anti 5-Methylcytosine mAb (Clone 5MC-CD, Biotin-conjugated)

Cosmo Bio LTD

Catalog No: BAM-51-004-EX

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Application: WB, ICC, IF 
Clonality: Monoclonal 
Conjugation: Biotin 
Host: Mouse 
Purification: IgM 

DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently
removed without changing the original DNA sequence. Therefore it is part of the epigenetic code and
is also the most well characterized epigenetic mechanism. DNA methylation results in addition of a
methyl group to DNA — for example, to the number 5 carbon of the cytosine pyrimidine ring — which
involves reduction in gene expression. In adult somatic tissues, DNA methylation typically occurs in
a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells. This
hybridoma has been constructed by Prof. H. Sano.
 
Applications
1) Immunocytochemistry (Figure below and Ref.1 & 2) (~50-100 fold dilution)
2) Immuno-blotting detection of DNA with 5-methylcytosine on nitrocellulose (Ref. 3 & 4) (~1000
fold dilution) Not tested with other application
Immunogen: 5-Methylcytosine conjugated to bovine serum albumin (Ref 3)
Reactivity: DNA with 5-Methylcytosine (methylated DNA), any species
Isotype: IgM
Form: Purified biotinylated IgM, 1 mg/ml in PBS with 50% glycerol, filter-sterilized, azide and carrier
free.
Storage: shipped at 4℃ or -20℃, and upon arrival, aliquot and store at -20℃.
 
References: This product has been used in references 1-4 (& many more publications).
1. Sharif J et al “The SRA protein Np95 mediates epigenetic inheritance by recruiting Dmnt1 to
methylated DNA” Nature 450: 908-912 (2007) PMID: 17994007
2. Nishiyama R et al “A chloroplast-resident DNA methyltransferase is responsible for
hypermethylation of chloroplast genes in Chlamydomonas maternal gametes” PNAS 99:
5925-5930 (2002) PMID: 11983892
3. Sano H et al “Detection of heavy methylation in human repetitive DNA subsets by a monoclonal
antibody against 5-methylcytosine” Biochim Biophys Acta 951:157-65 (1988) PMID: 2847796
4. Sano H et al “Identification of 5-methycytosine in DNA fragment immobilized on nitrocellulose
paper “PNAS 77:3581-3585 (1980) PMID: 6251470