CARD HyperOva® Ultra-Superovulation Reagent

CARD HyperOva® - a better superovulation reagent 
CARD HyperOva® FD - a better superovulation reagent in a new, convenient freeze-dried format 

 

 

CARD HyperOva® is an ultra-superovulation reagent that induces superovulation at higher efficiency than conventional methods with PMSG-hCG. Treatment with CARD HyperOva® yields 3-4 times more oocytes on average with C57BL/6J mice and is also effective with other strains. Moreover, further gains in efficiency can be realized by using CARD Medium during the IVF procedure.

---

Why Use CARD HyperOva®?

Reduce Animal Use (contribute to the 3Rs of animal welfare: Replacement, Reduction, Refinement)
Using CARD HyperOva® can reduce the number of oocyte donor mice by a factor of 1/3-1/4.

Optimize creation and Preservation of genetically modified mice. 
More ova from less mice using CARD HyperOva® facilitates in vitro fertilization and embryo transfer.

Get more done!
More ova from less mice using CARD HyperOva® enables the large-scale preparation of embryos. 

Swollen oviduct following superovulation.	Mice from superovulated ova.
CARD HyperOva® is a solution. Ships and stores frozen.	CARD HyperOva® FD is freeze-dried. Ships and stores at 4 C.

---

CARD HyperOva® Performance 

Table 1. CARD HyperOva® Performance with Various Mouse Strains

* 7.5 IU of CARD HyperOva® or PMSG was injected to four weeks old female mice, then 7.5 IU of hCG was administered 48 hours later. Thereafter all ova obtained 17 hours after hCG injection were collected.
* The in vitro fertilization rate is defined as the number of 2-cell embryos divided by the total number of ova (mean±SD).
* Data cited from a paper published by Prof. Naoki Nakagata and Toru Takeo (Center for Animal Resources and development, Kumamoto University)  

No.	Strain	Injection	Number of Oocyte Donors	Number of Inseminated Oocytes	Average Number of Ovulated Oocytes/Female	No. of Fertilized eggs	Fertilization Rate (%)
1	C57BL/6J	eCG	10	277	27.7±5.4	267	96.4±3.2
		HyperOva	10	1072	107.2±22.7	963	89.8±3.7
2	BALB/c ByJ	eCG	10	293	29.3±8.9	213	73.4±11.3
		HyperOva	10	903	90.3±14.5	680	76.4±9.0
3	C3H/HeJ	eCG	10	286	28.6±8.4	251	87.8±22.6
		HyperOva	10	520	52.0±18.1	446	85.8±15.5
4	DBA/2J	eCG	10	225	22.5±7.8	194	86.2±11.0
		HyperOva	10	688	68.8±13.6	592	86.0±4.5
5	FVB/NJ	eCG	10	168	16.8±3.3	159	94.6±6.7
		HyperOva	10	256	25.6±5.9	239	93.3±6.6
6	CD1	eCG	10	202	20.2±8.0	146	72.3±16.0
		HyperOva	10	337	33.7±9.9	269	79.8±19.6

 Figure 1. Number of Oocytes obtained from Various Mouse Strains using CARD HyperOva® 

---

CARD HyperOva® and CARD HyperOva® FD

Ordering Information

 For MOUSE Superovulation

Product Name Information	Catalog Number	Size
CARD HyperOva®  - the premier mouse superovulation reagent	KYD-010-06-EX	1 x 600 μl
	KYD-010-06-EX-X5	5 x 600 μl
	KYD-010-06-EX-X25	25 x 600 μl
	KYD-010-06-EX-X50	50 x 600 μl
	KYD-010-EX	1 x 1 ml
	KYD-010-EX-X5	5 x 1 ml
	KYD-010-EX-X25	25 x 1 ml
	KYD-010-EX-X50	50 x 1 ml
CARD HyperOva FD same great performance in a freeze-dried format for convenient storage and reduced shipping costs.	KYD-015-EX	1 x 2 ml
	KYD-015-EX-X5	5 x 2 ml
	KYD-015-EX-X25	25 x 2 ml
	KYD-015-EX-X50	50 x 2 ml

---

HyperOva® Frequently Asked Questions 

Q1	After opening the CARD HyperOva®, can it be subdivided and stored frozen?
A1	No. We do not recommend refreezing after thawing because we cannot guarantee the quality.
Q2	I didn't ovulate much. Is it defective?
A2	For C57BL/6J mice, administration is recommended between 26 and 30 days of age (birth date is considered as 0 days of age). Due to the nature of the product, the effects of this product may not be fully obtained at certain ages. In addition, the optimum age in weeks may differ depending on the strain. For special strains and ages, please contact us individually.
Q3	A sediment is deposited on the product. Are there any problems with using it?
A3	In rare cases, ingredients may precipitate. Precipitates are not the main component of this product. Avoid deposits during use.
Q4	Is CARD HyperOva ® effective against hyperovulation in rats?
A4	This product is manufactured based on mouse antigens. The effect of superovulation in rats has been confirmed to some extent, though not as much as in mice. In the case of rats, we administer 0.4-0.8mL up to 5-week-old rats, and 48-56 hours later, hCG (15-30IU/head) is administered, and eggs are collected the next morning. Please note that the reactivity differs depending on the rat strain
Q5	Is CARD HyperOva® a mixture of equine CG and goat anti-inhibin antiserum?
A5	Yes, it is mixed.
Q6	Tell me more about the horse gonadotropin.
A6	The horse gonadotropin in CARD HyperOva® is purified from a horse born and raised in Japan.
Q7	What is the country of origin for the goat?
A7	The goat is a male Saanen goat born and raised in Japan.
Q8	Did the goat co-mingle with any other livestock?
A8	No. Only with other goats.
Q9	Is the antibody unprocessed goat serum?
A9	The goat serum is diluted 2x with physiological saline. The horse gonadotropin is also dissolved in saline.
Q10	For the goat anti-mouse inhibin antiserum, what is the source of the immunogen?
A10	The immunogen is a synthetic peptide.
Q11	I can see a clump/precipitate/sediment in the vial.
Q12	Such imperfections do not diminish the effectiveness of CARD HyperOva®. Please avoid the clumps and use only the solution.
Q13	Can the product in 1ml vials be refrozen after being thawed if we don't need all of it at the moment?
A13	Once the 1ml vial is opened, please use it to administrate intraperitoneally all at once. 0.1mL - 0.2 mL can be administered to each mouse.
Q14	Will the product work after storage  at 4°C for one week instead of -20°C ?
A14	We cannot guarantee the quality under this storage condition. The CARD HyperOva may lost activity and we cannot recommend using it.

HyperOva® FD Frequently Asked Questions

Q1	Does the CARD HyperOva ® FD have an expiration date?
A1	Yes. As of January 2020, it is two years from the manufacture date. Please use it within the expiration date.
Q2	What is the difference between conventional CARD HyperOva ® and CARD HyperOva ® FD?
A2	CARD HyperOva ® FD is manufactured with a new formulation suitable for lyophilization. Conventional products needed to be stored at -20°C to -80°C, but this product can be stored at 4°C.
Q3	Are there any difference in the superovulation performance of CARD HyperOva ® FD compared to conventional products?
A3	Please expect the same superovulation results as the conventional CARD HyperOva ®
Q4	What should I do for long-term storage after thawing CARD HyperOva ® FD?
A4	Aliquot and store at or below -20°C. Please thaw before use. The superovulation effect has been confirmed to be maintained for about 3 months after the product is thawed or frozen.

---

Key References

NEW Synchronization of the ovulation and copulation timings increased the number of in vivo fertilized oocytes in superovulated female mice. Nakao S, Ito K, Sugahara C, Watanabe H, Kondoh G, Nakagata N, et al. (2023), PLoS ONE (2023) 18(2): e0281330. PMID: 36745586

Immunotherapy using inhibin antiserum enhanced the efficacy of equine chorionic gonadotropin on superovulation in major inbred and outbred mice strains.
Takeo T, Nakagata N. Theriogenology. 2016 Sep 15;86(5):1341-6. PMID: 27242176

Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice.
Takeo T, Nakagata N. PLoS One. 2015 May 29;10(5):e0128330. PMID: 26024317

  

 

Pups following superovulation with CARD HyperOva®