Density Gradient Media - Cosmo Bio USA
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Much of modern cell and molecular biology relies on the isolation of pure, functionally intact populations of cells, subcellular organelles and macro-molecules in high yield and both research and clinical applications provide an impetus for the development of improved separation technology.

The most commonly used method of separating suspensions of mixed populations of biological particles is centrifugation in density gradients - and the choice of medium used to form the gradients is critical for the retention of particles function. Since the early sixties Nycomed have been developing and producing iodinated compounds as X-ray imaging agents. It was recognized early on that these compounds were also well suited to the formation of density gradients and offered new opportunities for improved methods of separating biological particles, especially osmotically-sensitive cells and subcellular organelles. Nycomed has therefore been actively involved over the last 30 years in the research and development of density gradient media.

ProductDescriptionProduct Documents 

OptiPrep™
(iodixanol solution)

Iodixanol: 60% (w/v) 
Density: 1.320 ± 0.001 g/ml 
Osmolality: approx. 170 mOsm 
Endotoxins: < 1.0 EU/ml

The Optimal Density Gradient Media

OptiPrep is a sterile and endotoxin tested multi-purpose medium based on iodixanol. Unlike any of these other gradient media, iodixanol (as an X-ray imaging agent) has been clinically tested and is manufactured under strict and EU cGMP compliance.

OptiPrep™ Leaflet

OptiPrep™ Package Insert

OptiPrep™ MSDS

Optiprep™ rAAV references

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Nycodenz™
(iohexol powder)

A Universal Density Gradient Media

Nycodenz® is an off-white powder, freely soluble in water. Solutions up to 80% (v/w) with a density of 1.426 g/ml can be prepared. 

Nycodenz™ Leaflet

Nycodenz™ Package Insert

Nycodenz™ MSDS

 Description

Lymphoprep™

Sodium diatrizoate: 9.1% (w/v)
Polysaccharide: 5.7% (w/v)
Density: 1.077 ± 0.001 g/mL
Osmolality: 280 ± 15 mOsm
Endotoxins:

Ready-Made Solution for Isolating Human Mononuclear Cells (PBMC). GMP compliant and ISO 13485 certified.

Lymphohoprep™ is a ready-made, sterile and endotoxin tested solution for the isolation of human mononuclear cells.

Lymphoprep™ Leaflet

Lymphoprep™ Package Insert

Lymphoprep™ MSDS

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Lymphoprep Tube™

Convenient Fritted Centrifuge Tubes Prefilled with Lymphoprep™. 
GMP compliant and ISO 13485 certified.

Lymphoprep™ Tube is a sterile tube in which the Lymphoprep™ is contained below a plastic filter disc. This allows diluted blood to be poured simply and directly into the tube, the disc preventing any mixing with the separation medium. 

Lymphoprep™ Tube Leaflet

Lymphoprep™ Tube Package Insert

Lymphoprep™ Tube MSDS

 

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Diatrizoic Acid (aka Hypaque™) - the major component of Lymphoprep™. Hypaque is a trademark of GE Healthcare.

Polymorphprep™

Sodium diatrizoate: 13.8% (w/v).
Dextran 500: 8.0% (w/v).
Density: 1.113 ± 0.001 g/ml.
Osmolality: 445 ± 15 mOsm.
Endotoxins: < 1.0 EU/ml.  

Ready-Made Solution For Isolation of Human Polymorphonuclear Cells

Polymorphprep™ is a ready-made, sterile and endotoxin tested solution for the isolation of human polymorphonuclear leucocytes (granulocytes).

Polymorphprep™ Leaflet

Polymorphprep™ Package Insert

Polymorphprep™ MSDS

Polymorphprep™ Application Sheet

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Polysucrose™ 400

Versatile Non-Ionic, Hydrophillic, Water-Soluble Powder for Density Gradient Separations And Other Applications

Polysucrose™ 400 is a synthetic high molecular weight polymer made by the copolymerization of sucrose and epichlorohydrin. The molecules have a branched structure with a high content of hydroxyl groups giving a good solubility in aqueous solutions. The product is similar to Ficoll® 400 from GE Healthcare.

Polysucrose™ 400 Leaflet

Polysucrose™ 400 MSDS

 

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  • Lymphoprep

      Lymphoprep™ is a ready-made, sterile, and endotoxin tested solution that has been used to isolate mononuclear cells for more than 35 years. Mononuclear cells (monocytes and lymphocytes) have a lower buoyant...

  • Lymphoprep Tube

      Lymphoprep™ Tube is a sterile tube in which the Lymphoprep™ is contained below a plastic filter disc (barrier frit). This allows blood to be poured simply and directly into the tube, the disc preventing...

  • Nycodenz

      Nycodenz® is a universal density gradient medium provided as an non-ionic, non-toxic powder that is freely soluble in water.  Solutions up to 80% (v/w) with a density of 1.426 µg/ml can be...

  • OptiPrep

      OptiPrep™ is a sterile and endotoxin tested multi-purpose medium based on iodixanol. Unlike any of these other gradient media, iodixanol (as an X-ray imaging agent) has been clinically tested and is...

  • Polymorphprep

      Polymorphprep™ is a ready-made, sterile, and endotoxin tested solution used to isolate polymorphonuclear leukocytes.  With the exception of the basophils, polymorphonuclear leukocytes (PMNs) have...

  • Polysucrose 400

      Polysucrose™ 400 is a synthetic high molecular weight polymer made by the copolymerization of sucrose and epichlorohydrin. The molecules have a branched structure with a high content of hydroxyl groups giving...