Antibody - Primary Antibodies - Disease - Alport Syndrome - Cosmo Bio USA
Close

Site Information

Alport Syndrome Research Antibodies

Collagen 4 Alpha-Chain Rat Monoclonal Antibodies
(Developed At The Shigei Medical Research Institute)

 Take 10% OFF the list price of all collagen IV α-chain antibodies through September 30, 2018. 

sge-02.jpg
sge-03.jpg

For years, our popular fluorescent monoclonal antibody cocktail has been a staple for Alport Syndrome and related research. But until now, the three antibodies comprising the cocktail were not for sale individually.

You Asked, we Listened.  Now, rat monoclonals anti-collagen 4 α5(IV) [clone H52], anti COL4 α5(IV) [clone B51] and anti-COL4 α2(IV) [clone H25] are available for sale in both native and conjugated forms.  Thank you

 

Anti-Collagen 4 Alpha Chain Rat Monoclonal Antibodies

Rat Monoclonal Clone No.  H53 B51 H25 H52
Collagen Chain Specificity COL4 α5(IV) COL4 α5(IV) COL4 α2(IV) COL4 α5(IV)
Specificity Imperfection III
IDVEF at aa. 251-255 of alpha 5
NC1 domain Imperfection XIII
EAIQP at aa. 675-679 of alpha 2.
NC1 domain
SKPQSETL
Catalog Number,
unconjugated

SGE-C453

web page

datasheet

SGE-C451

web page

datasheet

SGE-C425

web page

datasheet

SGE-C452

web page

datasheet

Catalog Number,
FITC Conjugated

SGE-CFT453

web page

datsheet

SGE-CFT451

web page

datasheet

 n/a  n/a

Catalog Number,
Texas Red Conjugate

 n/a  n/a

SGE-CFT-425

web page

datasheet

 n/a

Component of Alport Cocktail
(cat no. SGE-CFT45325)

web page

datasheet

ALPORT APPLICATION EXAMPLES

yes yes yes no
Reactivity human human human hu, rat, rbt, gp, dog, cow
Host rat rat rat rat
Isotype G2a/k G2a G1/k G2b/k
Applications WB, IF WB, IF WB, IF WB, IF
Orignal Reference Kagawa et. al. Borza et. al. Kagawa et. al. Sado et. al.
     

References 

Borza DB, Bondar O, Ninomiya Y, Sado Y, Naito I, Todd P, Hudson BG. The NC1 domain of collagen IV encodes a novel network composed of the alpha 1, alpha 2, alpha 5, and alpha 6 chains in smooth muscle basement membranes. J Biol Chem. 2001 Jul 27;276(30):28532-40. Epub 2001 May 25. PubMed PMID: 11375996.

Kagawa M, Kishiro Y, Naito I, Nemoto T, Nakanishi H, Ninomiya Y, Sado Y. Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport's syndrome. Nephrol Dial Transplant. 1997 Jun;12(6):1238-41. PubMed PMID: 9198058.

Naito I, Kawai S, Nomura S, Sado Y, Osawa G. Relationship between COL4A5 gene mutation and distribution of type IV collagen in male X-linked Alport syndrome. Japanese Alport Network. Kidney Int. 1996 Jul;50(1):304-11. PubMed PMID: 8807602.

Ninomiya Y, Kagawa M, Iyama K, Naito I, Kishiro Y, Seyer JM, Sugimoto M, Oohashi T, Sado Y. Differential expression of two basement membrane collagen genes, COL4A6 and COL4A5, demonstrated by immunofluorescence staining using peptide-specific monoclonal antibodies. J Cell Biol. 1995 Sep;130(5):1219-29. PubMed PMID: 7657706PubMed Central PMCID: PMC2120565.

Sado Y, Kagawa M, Kishiro Y, Sugihara K, Naito I, Seyer JM, Sugimoto M, Oohashi T, Ninomiya Y. Establishment by the rat lymph node method of epitope-defined monoclonal antibodies recognizing the six different alpha chains of human type IV collagen. Histochem Cell Biol. 1995 Oct;104(4):267-75. PubMed PMID: 8548560.

Yoshioka K, Hino S, Takemura T, Maki S, Wieslander J, Takekoshi Y, Makino H, Kagawa M, Sado Y, Kashtan CE. Type IV collagen alpha 5 chain. Normal distribution and abnormalities in X-linked Alport syndrome revealed by monoclonal antibody. Am J Pathol. 1994 May;144(5):986-96. PubMed PMID: 8178947PubMed Central PMCID: PMC1887361.

SEE ALL COLLAGEN 4 ANTIBODIES BELOW