One-Step RT-PCR Kit
                 

Features
1.  Simple to use
2.  One-step set-up
3.   2x RT-PCR Mix optimized for as little as 100pg Total RNA
4.  Contains ultra-pure dNTPs

Applications
1.  Quantitative PCR
2.  Gene expression analysis
3.  Gene cloning

Description The One-Step RT-PCR Kit has been designed for highly sensitive one-step RT-PCR reactions using any RNA template. The kit employs an enzyme formulation, which includes hot-start DNA polymerase and reverse transcriptase. The One-Step RT-PCR Kit provides highly specific reverse transcription and PCR in a single tube, using gene-specific primers on either total RNA or mRNA. The kit is provided with RNase inhibitor to protect template RNA from degradation. The proprietary buffer is highly optimized and balanced, leading to outstanding results. The kit is ideal for the synthesis of double-stranded cDNA products for subsequent cloning, sequencing, expression, or transcription analysis.

The kit can be used with starting amounts of RNA template from 100pg to 2μg. After cDNA synthesis has been performed, the reaction is heated to 95°C for 10 minutes to inactivate the reverse transcriptase, and simultaneously to activate the hot-start DNA polymerase included. The polymerase improves specificity by eliminating the presence of non-specifics, primer-dimers, and mis-primed products

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Storage Conditions  The One-Step RT-PCR Kit can be stored for 6 months at -20°C.

Shipping Conditions  On Dry Ice or Blue Ice