CITATIONS |
|
|
| 2009 |
|
|
Kuroha K., Horiquchi N., Aiba H., Inada T. Analysis of nonstop mRNA translation in the absence of tmRNA in Escherichia coli. Genes Cells. 2009 May 14. PMID: 19469882
i)Experiments using PURESYSTEM revealed that ribosomes translating nonstop mRNAs were dissociated from the 3' end of mRNA, and the peptidyl-tRNA was only weakly hydrolyzed in the monosome.
|
|
|
|
|
|
Pfeiffer V., Papenfort K., Lucchini S., Hinton J. C. D., Vogel J., Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation. Nature Structural & molecular Biology. 2009. 16(8):840-846. PMID: 19620966.
i)Puresystem for bacterial small RNA research.
|
|
|
|
|
|
Zheng Y., Posfai J., Morgan R.D., Vincze T., Roberts R.J. Using shotgun sequence data to find active restriction enzyme genes Nucleic Acids Res. 2009 Jan;37(1):e1. Epub 2008 Nov 6. PMID: 18988632
i)PURESYSTEM was used to assay the DNA cleavage activity of the products of the predicted ORFs. |
|
|
| |
|
|
| 2008 |
|
|
Terashima H., Abe-Yoshizumi R., Kojima S., Homma M. Cell-free synthesis of the torque-generating membrane proteins, PomA and PomB, of the Na+-driven flagellar motor in Vibrio alginolyticus. 2008 Nov;144(5):635-42. Epub 2008 Sep 6. J Biochem. PMID: 18776205
i)Synthesized PomA and PomB usingPURESYSTEM in the presence of liposomes.
|
|
|
| |
|
|
| Maki K., Uno K., Morita T., Aiba H., RNA, but not protein partners, is directly responsible for translational silencing by a bacterial Hfq-binding small RNA. Proc Natl Acad Sci USA. 2008 Jul 29;105(30):10332-7. Epub 2008 Jul 23. PMID: 18650387 |
|
|
| |
|
|
Yamano K., Yatsukawa Y., Esaki M., Hobbs A.E., Jensen R.E., Endo T., Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import. J. Biol Chem. 2008 Feb 15;283(7):3799-807. Epub 2007 Dec 6. PMID: 18063580
i) Synthesis of radiolabeled proteins for mitochondrial import asays. |
|
|
| |
|
|
Urban J., Vogel J., Two Seemingly Homologous Noncoding RNAs Act Hierarchically to Activate glmS mRNA Translation. PLoS Biology. 2008 5(3)e64:1-12. PMID: 18351803
i)Puresystem for bacterial small RNA research. |
|
|
| |
|
|
| 2007 |
|
|
| Murtas G, Kuruma Y, Bianchini P, Diaspro A, Luisi PL., Protein synthesis in liposomes with a minimal set of enzymes. Biochem Biophys Res Commun. 2007 Nov 9;363(1):12-7. PMID: 17850764 |
|
|
| |
|
|
| Murtas G., Question 7: construction of a semi-synthetic minimal cell: a model for early living cells. Orig Life Evol Biosph. 2007 Oct;37(4-5):419-22. PMID: 17668286 |
|
|
| |
|
|
| Kuruma Y., Question 7: biosynthesis of phosphatidic acid in liposome compartments - toward the self-reproduction of minimal cells. Orig Life Evol Biosph. 2007 Oct;37(4-5):409-13. PMID: 17653611 |
|
|
| |
|
|
| Sharma CM, Darfeuille F, Plantinga TH, Vogel J., A small RNA regulates multiple ABC transporter mRNAs by targeting C/A-rich elements inside and upstream of ribosome-binding sites. Genes Dev. 2007 Nov 1;21(21):2804-17. PMID: 17974919 |
|
|
| |
|
|
| 2006 |
|
|
Kaiser CM, Chang HC, Agashe VR, Lakshmipathy SK, Etchells SA, Hayer-Hartl M, Hartl FU, Barral JM. Real-time observation of trigger factor function on translating ribosomes. Nature. 2006 Nov 23;444(7118):455-60. PMID: 17051157
i) Coupled transcription and translation with PURESYSTEM.
ii) Examining function of a factor not part of PURESYSTEM.
iii) 35-S-Met protein labeling using PURESYSTEM. |
|
|
| |
|
|
Shimizu Y, Ueda T. SmpB triggers GTP hydrolysis of elongation factor Tu on ribosomes by compensating for the lack of codon-anticodon interaction during trans-translation initiation. J Biol Chem. 2006 Jun 9;281(23):15987-96. PMID: 16601123
i) Preparation of Pre-trans-translation Complex (stalled ribosomes).
ii) Incorporation of 14C-alanine into protein.
iii) investigations of trans-translation initiation. |
|
|
| |
|
|
| Murakami H, Ohta A, Ashigai H, Suga H. A highly flexible tRNA acylation method for non-natural polypeptide synthesis. Nat Methods. 2006 May;3(5):357-9. PMID: 16628205 |
|
|
| |
|
|
Tomic S, Johnson AE, Hartl FU, Etchells SA. Exploring the capacity of trigger factor to function as a shield for ribosome bound polypeptide chains. FEBS Lett. 2006 Jan 9;580(1):72-6. PMID: 16359675
i) Puresystem delta RF1, RF2 used to photocrosslink translation products with ribosomes. |
|
|
| |
|
|
| Itoh H, Kawazoe Y, Shiba T. Enhancement of protein synthesis by an inorganic polyphosphate in an E. coli cell-free system. J Microbiol Methods. 2006 Feb;64(2):241-9. PMID: 15979174 |
|
|
| |
|
|
Ogawa A, Sando S, Aoyama Y. Termination-free prokaryotic protein translation by using anticodon-adjusted E. coli tRNASer as unified suppressors of the UAA/UGA/UAG stop codons. Read-through ribosome display of full-length DHFR with translated UTR as a buried spacer arm. Chembiochem. 2006 Feb;7(2):249-52. PMID: 16381047
i) Application for ribosome display. Stable complex of ribosome with mRNA and its translated protein. |
|
|
| |
|
|
| Ying BW, Taguchi H, Ueda T. Co-translational binding of GroEL to nascent polypeptides is followed by post-translational encapsulation by GroES to mediate protein folding. J Biol Chem. 2006 Aug 4;281(31):21813-9. PMID: 16754671 |
|
|
| |
|
|
| 2005 |
|
|
| Kuruma Y, Nishiyama K, Shimizu Y, Muller M, Ueda T. Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins. Biotechnol Prog. 2005 Jul-Aug;21(4):1243-51. PMID: 16080708 |
|
|
| |
|
|
Fukushima K, Ikehara Y, Yamashita K. Functional role played by the glycosylphosphatidylinositol anchor glycan of CD48 in interleukin-18-induced interferon-gamma production. J. Biol Chem. 2005 May 6;280(18):18056-62. PMID: 15760905
i) Synthesis of radio isotope-labelled protein.
ii) Synthesizing labelled protein in the presence of 35S-Met. |
|
|
| |
|
|
Yano M, Okano HJ, Okano H. Involvement of Hu and heterogeneous nuclear ribonucleoprotein K in neuronal differentiation through p21 mRNA post-transcriptional regulation. J Biol Chem. 2005 Apr 1;280(13):12690-9.
i) Synthesis of radio isotope-labelled protein.
ii) Synthesizing labelled protein in the presence of 35S-Met. |
|
|
| |
|
|
Ying BW, Taguchi H, Kondo M, Ueda T. Co-translational involvement of the chaperonin GroEL in the folding of newly translated polypeptides. J Biol Chem. 2005 Mar 25;280(12):12035-40. PMID: 15664980
i) Analysis of protein maturation mechanism.
ii) Effect of added GroE could be studied, since PURESYSTEM does not contain molecular chaperones. |
|
|
| |
|
|
Tanaka R, Mizukami M, Tokunaga M. Novel processing and localization of catA, ccdA associated thiol-disulfide oxidoreductase, in protein hyper-producing bacterium Brevibacillus choshinensis. Protein Pept Lett. 2005 Jan;12(1):95-8. PMID: 15638809
i) Production of protein standard.
ii) Standard of precursor protein for processing. |
|
|
| |
|
|
| Yano M, Okano HJ, Okano H., Involvement of Hu and heterogeneous nuclear ribonucleoprotein K in neuronal differentiation through p21 mRNA post-transcriptional regulation., J Biol Chem. 2005 Apr 1;280(13):12690-9. PMID: 15671036 |
|
|
| |
|
|
| 2004 |
|
|
| Ying BW, Taguchi H, Ueda H, Ueda T. Chaperone-assisted folding of a single-chain antibody in a reconstituted translation system. Biochem Biophys Res Commun. 2004 Aug 20(4):1359-64. PMID: 15303282
i) Synthesis of single chain Fv of antibody.
ii) Active form of scFv synthesized in the presence of molecular chaperones under an oxidative state. |
|
|
| |
|
|
Asai T, Takahashi T, Esaki M, Nishikawa S, Ohtsuka K, Nakai M, Endo T. Reinvestigation of the requirement of cytosolic ATP for mitochondrial protein import. J Biol Chem. 2004 May 7;279(19):19464-70. PMID: 15001571
i) Characterization of ATP dependent factor required for ADP/ATP carrier mitochondrial import. |
|
|
| |
|
|
Kawano M, Suzuki S, Suzuki M, Oki J, Imamura T. Bulge- and basal layer-specific expression of fibroblast growth factor-13 (FHF-2) in mouse skin. J Invest Dermatol. 2004 May;122(5):1084-90. PMID: 15140207
i) Production of control to test antibody specificity. |
|
|
| |
|
|
Udagawa T, Shimizu Y, Ueda T. Evidence for the translation initiation of leaderless mRNAs by the intact 70 S ribosome without its dissociation into subunits in eubacteria. J Biol Chem. 2004 Mar 5;279(10):8539-46. PMID: 14670970
i) Analysis of protein synthesis mechanism.
ii) Observing synthesis initiation steps by changing constituting factors. |
|
|
| |
|
|
| 2003 |
|
|
Ying BW, Suzuki T, Shimizu Y, Ueda T. A novel screening system for self-mRNA targeting proteins. J Biochem (Tokyo). 2003 Apr;133(4):485-91. PMID: 12761296
i) Screening mRNA-binding protein. |
|
|
| |
|
|
| 2002 |
|
|
Shimizu Y, Ueda T. The role of SmpB protein in trans-translation. FEBS Lett. 2002 Mar 6;514(1):74-7. PMID: 11904185
i) Analyzing the role of a translation regulatory factor. |
|
|
| |
|
|
| 2001 |
|
|
Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T., Cell-free translation reconstituted with purified components. Nat Biotechnol. 2001 Aug;19(8):751-5. PMID: 11479568
i) Origninal description of PURESYSTEM. |
|
|
| |
|
|
Swartz J. A PURE approach to constructive biology. Nat Biotechnol. 2001 Aug;19(8):732-3. PMID: 11479561
i) Comment on original description of PURSYSTEM. |
|
|
| |
|
|
REVIEWS |
|
|
Shimizu Y, Kanamori T, Ueda T. Methods. 2005 Jul;36(3):299-304. PMID: 16076456
i) Protein synthesis by PURE translation systems. |
|
|
| |
|
|
Shimizu Y, Kuruma Y, Ying BW, Umekage S, Ueda T. Cell-free translation systems for protein engineering. FEBS J. 2006 Sep;273(18):4133-40. Review. PMID: 16930131
i) Series of mini-reviews on cell free protein synthesis. |
|
|
