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Tools and Reagents for Molecular Biology

Protein Electrophoresis and Blotting Tools SimaSima Protein MW Marker Ladders
  • new SimaSima Molecular Weight Markers - High spec, high performance protein molecular weight ladders.
  • Can Get Signal Immunoreaction Enhancer Solution- Use as antibody diluent for Westerns, ELISAs, and dot blots. Increases S/N ratios. Compatible with colorimetric, chemiluminescent, and fluorecent detection methods.
  • Hybri-Bag - Heat sealable bags for hybridization and detection of blots. Comes in three formats: Soft, Hard, and Hard with Grid. Hard bags are autoclavable.
SimaSima
   
Molecular Cloning, PCR, RT-PCR, QPCR etc..
Amplification Plot
  • new! Single Primer RACE: "accura-expRACE Kit" - Perform 5' and 3' RACE with the same cDNA using only a single primer. Use double-stranded cDNA without adaptor ligation or phage vector. Efficiently isolate long, full-length cDNAs and cDNAs from rare mRNAs and splice variants.
  • new! 1st Strand cDNAs (Mouse and Rat) from normal and diseased tissues - Large selection of 1st strand cDNAs prepared from high quality RNA primed with oligo dT and transcrbed with MMLV reverse transcriptase. Quality controlled by gene specfic PCR.
  • Blend Taq and Blend Taq -Plus- Perform efficient DNA amplification with these
    proofreading blended polymerases. Blend Taq Plus provides the added convenience of a hot start formulation.
  • Taq DNA Polymerase (+dNTPs) - Thermostable DNA polymerase activity for PCR. Expressed in large quantities and highly purified.
  • Realtime PCR Master Mix and SYBR Green Realtime PCR Master Mix- Two ready to use QPCR master mixes, just add primers and template.  Available with or without SYBR Green I for use with unlabled or labled primer/probe assays.
  • One-Step RT-PCR Kit- Simple to use, easy one-step set-up. This kit is ideal for the synthesis of ds cDNA products for cloning, sequencing, expression, or transcription analysis.
  • dNTPs- Ultra-pure RNase-, DNase-, Nickase-free 100mM dNTPs, set of 4 vials. Use in PCR, cDNA synthesis, sequencing, labeling, etc.
  • 100bp Ready-to-Use DNA Ladder- Provides a range from 100bp to 1500bp with an intense 500bp band for orientation. Simply load 5ul per well and run the gel.
amplification plot
   
Purification
Magical Trapper
  • Magical Trapper- Multifuntional separation stand ideal for isolation of nucleic acids and proteins by magnetic beads.  Suitable for use in conjuction with MagExtractor® series of purification products.
  • MagExtractor®-PCR & Gel Clean Up - Purify dsDNA from reactions or agarose gels without Phenol/Chloroform. Isolated DNA is suitable for routine downstream applications. Use with manual Magical Trapper stand or with MFX automation.
  • MagExtractor®-Plasmid - Purify plasmid DNA from bacterial cultures without Phenol/Chloroform. Isolated plasmid is suitable for transformations and enzymatic reactions. Use with manual Magical Trapper stand or with MFX automation.
  • MagExtractor®-RNA- Purify total RNA from cells, tissues, yeast without Phenol/Chloroform. Isolated RNA is suitable for enzymatic reactions. Use with manual Magical Trapper stand or with MFX automation.
  • MagExtractor®-Genome- Purify genomic DNA from blood, cells, tissues without Phenol/Chloroform. Isolated genomic DNA is suitable for enzymatic reactions. Use with manual Magical Trapper stand or with MFX automation.
  • CellEase II - Fast and easy DNA extraction in 9 minutes without purification and separation. Kits are available for tissue, bacteria, and meat samples.
   
Bioseparation
Magnosphere MS300/Streptavidin
  • Magnoshere MS300/Streptavidin- Isolate biotinylated proteins or nucleotides using these superparamagnetic microparticles. Beads feature low non-specific binding and are free from enzyme activity. Applications include PCR, DNA hybridization, and enzyme immunoassays.
  • Magnoshere MS300/Carboxyl- Immobilize antibodies, proteins or nucleotides to the surface of these magnetic microparticles. Conjugated beads can be used enzyme immunoassays, immunoprecipitation, and nucleic acid hybridization.
  • Magnoshere MS300/Low Carboxyl- Immobilize antibodies, proteins or nucleotides to the surface of these magnetic microparticles.Conjugated beads can be used enzyme immunoassays, immunoprecipitation, and nucleic acid hybridization. Low Carboxyl beads feature ultra low non-specific binding and are ideal for LC-MS.
  • Sugar Chain Immobilized Gold Nano Particles- Useful for evaluating sugar chain-protein interactions in protein aggregation or inhibitory assays, dot blots, or protein isolation. Available with a variety of structurally defined sugar chains.
  • Heparin Gold Nano Particles- These gold nanoparticles are immorbilized with heparin, a sulfated polysacharride. Applications include: isolation of heparin binding molecules from crude lysates and probing dot blots.
   
Nucleic Acid Restriction and Modification Enzymes & Kits
Ligation high
   
Protein Modification, Cell Labeling
Clover Direct
   
Immunoblotting Accessories
PerfectHyb
  • PerfectHyb Hybridization Solution- Optimized for low expression genes. For use on Northern or Southern blots. No salmon sperm needed, hyb in only 1-2 hours.
  • Can Get Signal Immunoreaction Enhancer Solution- Use as antibody diluent for Westerns, ELISAs, and dot blots. Increases S/N ratios. Compatible with colorimetric, chemiluminescent, and fluorecent detection methods.
  • Hybri-Bag- Heat sealable bags for hybridization and detection of blots. Comes in three formats: Soft, Hard, and Hard with Grid. Hard bags are autoclavable.