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Homocysteine
thiolactonase 
Alfresa Auto HTLase serum homocysteine thiolactonase assay
HTLase in serum samples hydrolyzes gamma-thiobutyrolactone substrate to 4-mercaptobutyric acid. Reaction of 4-mercaptobutyric acid with Ellman's reagent (DTNB: 5,5'-dithiobis(2-nitrobenzoic acid) produces a colored thiolate ion (TNB: 5-thio-2-nitrobenzoic acid), read at 412 nm. |