Density Gradient Media

 Much of modern cell and molecular biology relies on the isolation of pure, functionally intact populations of cells, subcellular organelles and macro-molecules in high yield and both research and clinical applications provide an impetus for the development of improved separation technology.

The most commonly used method of separating suspensions of mixed populations of biological particles is centrifugation in density gradients - and the choice of medium used to form the gradients is critical for the retention of particles function. Since the early sixties Nycomed have been developing and producing iodinated compounds as X-ray imaging agents. It was recognized early on that these compounds were also well suited to the formation of density gradients and offered new opportunities for improved methods of separating biological particles, especially osmotically-sensitive cells and subcellular organelles. Nycomed has therefore been actively involved over the last 30 years in the research and development of density gradient 

 

Density Gradient Media Product Lineup

 

OptiPrep™(iodixanol solution)

  • The Optimal Density Gradient Media
  •  OptiPrep is a sterile and endotoxin tested multi-purpose medium based on iodixanol.
  • Unlike any of these other gradient media, iodixanol (as an X-ray imaging agent) has been clinically tested and is manufactured under strict and EU cGMP compliance.
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Nycodenz®(iohexol powder)

  • A Universal Density Gradient Media. Nycodenz® is an off-white powder, freely soluble in water.
  • Solutions up to 80% (v/w) with a density of 1.426 g/ml can be prepared.
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Lymphoprep™

  • Ready-Made Solution for Isolating Human Mononuclear Cells (PBMC). GMP compliant and ISO 13485 certified.
  • Lymphohoprep™ is a ready-made, sterile and endotoxin tested solution for the isolation of human mononuclear cells.
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Lymphoprep Tube™

  • Convenient Fritted Centrifuge Tubes Prefilled with Lymphoprep™.
  • GMP compliant and ISO 13485 certified.
  • Lymphoprep™ Tube is a sterile tube in which the Lymphoprep™ is contained below a plastic filter disc. This allows diluted blood to be poured simply and directly into the tube, the disc preventing any mixing with the separation medium.

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Polymorphprep™

  • Ready-Made Solution For Isolation of Human Polymorphonuclear Cells

  • Polymorphprep™ is a ready-made, sterile and endotoxin tested solution for the isolation of human polymorphonuclear leucocytes (granulocytes).

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Polysucrose™ 400

  • Versatile Non-Ionic, Hydrophillic, Water-Soluble Powder for Density Gradient Separations And Other Applications
  • Polysucrose™ 400 is a synthetic high molecular weight polymer made by the copolymerization of sucrose and epichlorohydrin. The molecules have a branched structure with a high content of hydroxyl groups giving a good solubility in aqueous solutions. The product is similar to Ficoll® 400 from GE Healthcare.
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  • Polymorphprep

    Serumwerk

    Polymorphprep

    Catalog No.(s): AXS-1114683

    Polymorphprep™ is a ready-made, sterile, endotoxin-tested, separation medium optimized for human granulocyte (polymorphonuclear leukocyte, PMN) isolation....

    Following centrifugation of whole undiluted human blood samples through Polymorphprep™, PMNs accumulate in a distinct band above pelleted erythrocytes and below mononuclear cells retained at the sample/medium interface....

    $110.00
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  • Lymphoprep

    Serumwerk

    Lymphoprep

    Catalog No.(s): AXS-1114544, AXS-1115758, AXS-1115757, AXS-1114545, AXS-1114547, AXS-1114546

    Lymphoprep™ is a ready-made, sterile, endotoxin-tested, separation medium for isolating human mononuclear cells (lymphocytes and monocytes), especially from blood. ...

    Relatively low buoyant density (below 1.077 g/ml) mononuclear cells are retained at the sample/medium interface while more dense erythrocytes and polymorphonuclear leukocytes (PMNs or granulocytes) sediment through the medium....

    $65.00 - $724.00
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  • Optiprep Iodixanol density gradient reagent

    Serumwerk

    OptiPrep - Density Gradient Media (Iodixanol)

    Catalog No.(s): AXS-1114542, AXS-1114542-5

    Catalog No.(s): AXS-1114542, AXS-1114542-5, AXS-1114542-10

    OptiPrep™ is a sterile, endotoxin-tested, multi-purpose separation medium of 60% iodixanol in water with a density of 1.32 g/ml....

    It is ideal for isolating viruses, subcellular organelles, membranes, macromolecules and cells, especially when controlling osmolality is a priority. ...

    Lowest Price...BY FAR....

    $172.00 - $1,548.00
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  • Lymphoprep Tube

    Serumwerk

    Lymphoprep Tube

    Catalog No.(s): AXS-1019818, AXS-1019817

    Lymphoprep™ Tube is a ready-made, sterile, endotoxin-tested, separation medium for isolating human mononuclear cells (lymphocytes and monocytes), especially from blood....

    It comprises a sterile plastic 50ml tube divided by a plastic frit into two compartments, the lower of which is prefilled with Lymphoprep™ which is also available in bulk (AXS-1114544)....

    $184.00 - $218.00
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  • Nycodenz

    Serumwerk

    Nycodenz

    Catalog No.(s): AXS-1002424

    Nycodenz® is a non-toxic, non-ionic, low osmolality, low viscosity, off-white powder, freely soluble in water for flexible generation of application-specific gradient separation media that can reach densities of up to 1.426 µg/ml (at 80% v/w)....

    It is used for isolating viruses, diverse subcellular organelles, membranes, macromolecules and cells....

    $726.00
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