Artemis TR-FRET
Microplate reader specifically designed for HTRF® applications
Catalog Number: FDK-K-101-EX

Artemis is a high performance but inexpensive microplate reader designed, dedicated and specifically tuned for reading assays built on CisBio’s innovative HTRF® (TR-FRET) assay technology. Read on to find out how Artemis can help you “take the fret out of TR-FRET”.

Artemis Features

• A top performing, value priced time-resolved fluorescence microplate reader
  dedicated for HTRF®. SBS Poster 2006. SBS Poster 2007. Artemis Brochure.
• Single photon counting detection system.
• Supports 96 and 384 well plates.
• HTRF® optimized for enhanced sensitivity, dynamic range, signal-to-noise ratio and cross-talk.
• Real time ratio calculation used to correct for medium interference (US patent 5,527,684).
• A precision instrument that’s build simple, tough, and dependable.
• Approved for HTRF® applications by Cisbio International.

Why HTRF®? The Genuine TR-FRET Chemistry TR-FRET  (time resolved-fluorescence energy transfer) is a detection technology that combines FRET chemistry with long emission half-life fluorophores. HTRF® (Homogenous Time Resolved Fluorescence) detection technology is based on TR-FRET but has several distinguishing properties.  HTRF® exclusively employs the extremely long half life donor fluorophore Europium, and its conjugation to a cryptate moiety which confers increased assay stability. Further, HTRF® employs a patented ratiometric measurement technique to correct for quenching and sample interference. Other HTRF® technology features include: Homogeneous assay format Low background Simplified assay miniaturization Tolerant of additives such as DMSO & EDTA Few false positive/false negatives Cell-based functional assay For complete information on HTRF®, visit Cisbio’s website at www.htrf.com.

Overview of ARTEMIS
Taking the "fret" out of TR-FRET

High sensitivity assay technologies demand a great reader, but you need one that won't break your budget. The Artemis TR-FRET microplate reader provides a low cost, high performance gateway into Cisbio's HTRF® assay technology. HTRF® is a beneficial extension of TR-FRET featuring homogenous assay protocols, extremely low backgrounds, and high sensitivity. Designed and built by Furuno Electric Company (Japan), a true world leader in marine electronics, the Artemis easily exceeds all criteria required by Cisbio for certified compatibility with HTRF® reagents. In addition, Furuno knows that precision instruments don't have to be delicate; the Artemis is built simple, tough, and rugged for years of dependable, low maintenance operation.

Selecting the Artemis reader takes the “fret” out of TR-FRET by simplifying your choices on the way to developing and running assays that meet your demanding specifications. Reagent selection is simplified because you go to Cisbio, the one expert source for all your HTRF® needs. Cisbio offers everything you’re likely to use from preconfigured assays for drug discovery targets, to a full selection of HTRF® toolbox reagents for assay development, to custom development programs for specialized applications. All reagents come matched and quality controlled. Then, the easy to use Artemis further simplifies your work by being fully dedicated to HTRF®, eliminating the need to select and change filters, and readily handling data collection, reduction, and analysis. Together, Artemis and Cisbio offer a practically turn-key solution to your TR-FRET assay needs.

Artemis Specifications

The Artemis is designed and manufactured to the highest quality specifications by:

For more product information from our manufacturer, please click the logo above to visit their website.

Artemis MircroPlateReader Software

The Artemis is completely controlled by the easy to use Windows based MicroPlateReader software. User adjustable assay set-up parameters include flash count (the number of light flashes applied to each assay well), delay time (time between flash and fluorescence detection), and integration time (period of fluorescence detection). Controls, blanks, and samples can be mapped anywhere on 96 or 384 well plates. Data acquisition, data analysis, and data reduction are used with Cisbio's patented ratiometric Calculations (US Patent 5,527,684), and are all conveniently handled by MicroPlateReader. The data output is automatically saved in comma separated value (csv) format for further calculation and analysis in a spreadsheet software such as Microsoft Excel.

	Instrument Settings Control Window
	Plate-Mapping Control Window
	Data Control Window

Minimum system requirements: Microsoft Windows XP operating system. Pentium 400 MHz (or AMD equivalent) processor, 128 MB of RAM, Super VGA (800 x 600) monitor, and at least 500 MB of available hard-disk space to store application software, csv files, plus added spreadsheet files depending on user.

Value-priced Artemis delivers high-end performance

From Independent and complete evaluations, the ARTEMIS reader was shown to be very well designed to obtain high quality
results with HTRF® assays, using either black or white plates for the same quality of results and excellent CVs (>3%).

	Artemis reading times can be shortened while maintaining excellent reproducibility. Data obtained using Cisbio Reader Control Kit reagents (Cat. No. 62RCLPEA). Click graph to enlarge.
	Sensitive detection of TNFa standards by Artemis. Cisbio TNFa Assay Kit (Cat. No. 62TNFPEB) standard curve prepared in assay diluent, read in 384-well small volume white plate. Delay time (D) and sample integration time (I) for Artemis readings are indicated. Click graph to enlarge.

Ordering Information

For additional information, please contact us.