Site Navigation

Cosmo Bio USA Homepage
Cosmo Bio USA All Products Index Page

 
 
 
Koma Biotech
PCR and Direct PCR Reagents and Kits

 
Koma Biotech Website (S. Korea)    
 
 
 
Koma Biotech ELISAs
Koma Biotech Direct PCR
Koma Biotech Forensic DNA Analysis
 
 
 

Direct PCR technology facilitates PCR amplification directly from small amount of samples without DNA extraction and purification. It eliminates any sample pretreatment steps, so it is very useful to save time and cost, protect samples from cross contamination or loss when dealing with multiple tissue or samples by eliminating tedious genomic DNA preparation. It allows direct PCR amplification from a variety of samples including whole blood, blood collected on card, saliva, bacteria, mouse tail, tissue, cultured cells or plant etc., by neutralizing DNA polymerase inhibitors present in the samples.

KOMA provides many different type of Direct PCR products. EzWay™ Direct PCR Buffer is compatible with most commercially available any DNA polymerases including Taq, Pfu, Pwo, Tth and even with any type of Hot Start Taq DNA polymerase, so you can use it together with your Taq systems that are already set up in your lab. Also, this Direct PCR Buffer is provided as a type of Direct PCR MasterMix which is already mixed with KOMA Taq enzymes . Just adding your primers and template to this MasterMix, you can go directly to PCR amplification.

Features of Direct PCR EzWay
Direct PCR
Conventional PCR
No DNA extraction required
Save Time and Cost
Less Risk of DNA Contamination
No Loss of Samples
Compatibility with any other Taq Enzymes

Applications of DIrect PCR include:

  • Viral infection / Molecular diagnostic test
  • Forensic DNA Analysis
  • Single cell diagnostic
  • Blood banking
  • Identity testing
  • Multiplex PCR / SNP detection / PCR-RFLP
  • Sequencing / Cloning
  • Laboratory Automatic PCR
 
     

 

Ordering Information
Koma Biotech Direct PCR Products

 
EzWay Direct PCR Buffers

EzWay™ Direct PCR Buffer enables the direct amplification of DNA fragments from a variety of samples including whole blood, blood collected on card, saliva, bacteria, mouse tail, tissue, cultured cells or plant eliminating the need for DNA purification, just by adding to the PCR mixture in place of general 10X PCR Buffer.

EzWay™ Direct PCR Buffer is compatible with most commercially available any DNA polymerases including Taq, Pfu, Pwo, Tth and even with any type of Hot Start Taq DNA polymerase. A one-step reaction using this EzWay™ Direct PCR Buffer enables easy PCR and minimizes the sample cross-contamination when dealing with multiple tissue or samples by eliminating tedious genomic DNA preparation.

Product Name (click for more info) Qty Catalog Number
(click for sample datasheet)
Cosmo Bio USA Price
EzWay Direct PCR Buffer (5x) 500ul
sufficient for
125 PCR reactions (20ul each)
50 PCR reactions (50ul each)

KBT-K0568001 $131
EzWay Direct PCR Buffer Set (5x)
kit contents
5x EzWay Direct PCR Buffer (500ul)
2.5x Direct Lysis Buffer I (1.5ml)
1x Direct Lysis Buffer II (1.5ml)
4x Magic Buffer (1ml)
1 set
sufficient for
125 PCR reactions (20ul each)
50 PCR reactions (50ul each)
KBT-K0568002 $147
 
EzWay Direct PCR MasterMixes
Product Name (click for more info) Qty Catalog Number
(click for sample datasheet)
Cosmo Bio USA Price
EzWay Direct Taq PCR MasterMix (2x)
premixed solution with Taq DNA Polymerase, dNTPs, MgCl2, PCR Direct Buffer. Contains red dye.
1ml KBT-K0568010 $131
EzWay Direct Taq PCR MasterMix w/o dye (2x)
premixed solution with Taq DNA Polymerase, dNTPs, MgCl2, PCR Direct Buffer. Does not contain red dye.
1ml KBT-K0568020 $131
 
EzWay Direct PCR Kits
Product Name (click for more info) Qty Catalog Number
(click for sample datasheet)
Cosmo Bio USA Price
EzWay Direct PCR ApoE Genotyping Kit
for simple ApoE genotyping of common alleles (E2 (Cys112/Cys158), E3 (Cys112/Arg158) and E4 (Arg112/Arg158) from whole blood without DNA extraction by direct PCR technology. Primer mixture is also suitable for one-step Multiplex PCR.
kit contents
2x EzWay Direct Hot Taq PCR MasterMix (650 ul)
2.x ApoE Primer Mix (500 ul)
Size Marker Soln, 112, 253, 308, 444, 514 bp (50 ul)
Positive Control DNA, E2/E4 type (20 ul)
50 tests KBT-K0568500 $816

EzWay Mouse Tail Direct PCR Kit
for DNA amplification directly from mouse tail without prior DNA extraction.
kit contents
2x EzWay Direct Taq PCR MasterMix (1 ml)
2.5x Mouse Tail Direct Lysis Buffer I (3 x 1.5ml)
4x Magic Buffer (1ml)
Positive Control (20 ul)
Control Primer (20 ul)

1 set KBT-K0568600 $230
EzWay Plant Direct PCR Kit
for DNA amplification from plant leaves without prior DNA extraction.
kit contents
2x EzWay Direct Taq PCR MasterMix (1 ml)
2.5x Plant Direct Lysis Buffer I (1.5ml)
4x Magic Buffer (1ml)
Positive Control (20 ul)
Control Primer (20 ul)
1 set KBT-K0568700 $230

 

Direct PCR Buffers
  Direct PCR Buffer Direct PCR Buffer Set
Cat. No KBT-K0568001 KBT-K0568002
Cosmo Bio USA Price    
  Direct PCR Buffer Direct PCR Buffer Set
Features Use Direct PCR Bin place of 10X PCR buffer for Direct PCR. Direct PCR buffer is provided with lysis buffers for treatment of hard tissue samples.
Contents Direct PCR Buffer Direct PCR Buffer, Direct Lysis Buffer I
Direct Lysis Buffer II, Magic Buffer
Sample Soft materials such as blood (whole, dried, on paper) buccal swab, saliva, sputum, urine, etc. Solid materials such as mouse tail, animal tissue, plant material, cultured cells, hair root, sperm, etc.

 

Direct PCR MASTERMIXes
Product Direct MasterMix Direct Hot Taq PCR MasterMix Direct Hot Universal qMasterMix
Cat. No KBT-K0568010 KBT-K0568210 KBT-K0569020
  Direct MasterMix Direct Hot Taq PCR MasterMix Direct Hot Universal qMasterMix
Features Direct PCR buffer is premixed with Taq, dNTP, MgCl2 and dye. Just add the template and primer to this MasterMix. Direct PCR buffer is premixed with Hot Taq, dNTP, MgCl2 and dye. Just add the template and primer to this MasterMix. It is useful for high specificity. Direct PCR buffer is premixed with Hot Taq, dNTP and MgCl2. Just add the template and primer to this MasterMix. It is for real time PCR without DNA extraction.
Component Direct Taq PCR Master Mix
Magic Buffer
Direct Hot Taq PCR Master Mix
Magic Buffer
Direct Hot Universal qMasterMix
Magic Buffer
Sample Most of sample type
(Lysis buffer should be purchased separately.)
Most of sample type
(Lysis buffer should be purchased separately.)
Most of sample type
(Lysis buffer should be purchased separately.)

 

 

Direct PCR KITS
Product EzWay™ Mouse Tail Direct PCR Kit EzWay™ Plant Direct PCR Kit
Cat. No KBT-K0568600 KBT-K0568700
  EzWay™ Mouse Tail Direct PCR Kit EzWay™ Plant Direct PCR Kit
Features Direct PCR buffer is premixed with Taq, dNTP, MgCl2 and dye. It is optimized for genotyping of transgenic mouse using tail or ear tissue without DNA extraction. Direct PCR buffer is premixed with Taq, dNTP, MgCl2 and dye. It is optimized for plant PCR without DNA extraction.
Component Direct Taq PCR Master Mix
Mouse Tail Lysis buffer
Magic Buffer
Direct Taq PCR Master Mix
Plant Lysis buffer
Magic Buffer
Sample Mouse tail, ear Plant leaves

 

 

 

 

 

       
       

Copyright 2016 Cosmo Bio USA, Inc. All right reserved