what's up.doc, No. 33,"Detection Reagents"

    

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Tools and Reagents for Optimizing Assay Development, Immunodetection
and Molecular Biology Procedures

New Fluorescent Dyes

IHC Detection Kits

 

IST Fluolid - Fluorescent dyes with unique performance characteristics and labelling efficiency. 

Fluolid dyes, for labeling of protein or oligoamines, feature high quantum yield in the solid state, no photo-bleaching, high stability towards light, temperature and pH, high incorporation rates, and very large Stoke's shifts. 

Ex/Em maxima:
Fluolid-W Orange 440/602
Fluolid-W Yellow 410/541
Fluolid-W Green 395/522

 

IST Fluolid
Unique Fluorescent Dye Set


Mouse spleen detected with N-Histofine® Simple Stain Max PO.

N-Histofine® MAX PO and Simple Stain Detection Kits 

Patented polymer technology ensures strong signal amplification and low background for high sensitivity staining.  Uneffected by endogenous biotin in tissue. Histofine kits are available for use with Human, Mouse and Rat tissue and mouse, rabbit, and goat primary antibodies.

Check our website for complete product listing and pricing.

Antibody Rxn Enhancer for Blotting and Assays

Antibody Rxn Enhancer
for Tissue Staining

Can Get™Signal Immunoreaction Signal Enhancer.  Improves antibody performance.

Simply use Can Get as antibody diluent for Westerns, ELISAs, and dot blots for dramtically increased s/n ratio in many systems. Compatible with colorimetric, chemiluminescent, and fluorescent detection methods. Enzyme labeled antibodies are not disturbed. Can Get Enhancer Trial set includes one solution optimized for 1° abs and one solution optimized for 2° abs.

TYB-NKB-101T, Can Get Signal Immunoreaction Enhancer Trial Set

Improved detection of phosphorylated Akt with insulin stimulated bovine cells.

Tricolor merged image using Can Get Signal® Immunostain solutions.

Can Get Signal® Immunostain Signal Enhancer. Improves antibody performance in IHC and ICC.

Optimized for cell staining, Can Get Immunostain Enhancer increases s/n ratio and decreases non-specific background. Fully compatible with enzyme and fluorescent dye-labeled  antibodies. Use for paraffin embedded or frozen sections. Try “A” type and “B” type (in Starter Set) included in Starter Set to find which works best in your system.


TYB-NKB-401, Can Get Signal Immunostain Enhancer Starter Kit

Improved Hybridization 

Gold Medal in Silver Staining

PerfectHyb® hybridization solution   - ready to use

A single solution containing a proprietary hybridization enhancer, PerfectHyb® is ideal for detecting low copy number genes.
Optimized for performance in Northerns and Southerns, including non-radioactive methods. Fast, convenient hybridization is complete in 1-2 hrs. No salmon sperm DNA required. Hybridize and wash at the same temperature.

TYB-HYB-101, PerfectHyb® Hybridization Solution,  250ml,

Northern Blot detection of transferrin receptor in HeLa cells.

1: PerfectHyb® , 2 hr                 
2: company A, 2 hr 
3: company B, 2 hr 
4: conventional methods, 16 hr

Fermentas SM1811 protein ladder from 4x to 1024x dilution, 3 ul each lane (not greater than 0.6 picograms protein/band at 1024x).

2D Silver Stain II   Kit
The finishing touch.

Even first time users can enjoy high sensitivity detection in as little as one hour with Cosmo's 2D-Silver Stain II Kit. 50-100 times more sensitive than Coumassie Blue or ethidium bromide for protein or DNA detection, respectively. Easy to use kit produces even staining, low background, and high contrast bands. Use for protein or DNA separated by PAGE or SDS-PAGE. Safe. No heavy metals except silver.

DCB-423413,  2D-Silver Stain II

Polymer-Based Assay Blockers And Enzyme Stabilizers 

Slide Incubation Chamber

Assay Blocking Reagents , 100% animal free synthetic polymer. 

These unique blocking reagents offer excellent performance, stability, and minimal lot-to-lot variation. NB4025 is especially efficient to prevent non-specific binding of Ig in human sera.B ased on similar synthetic polymers, Peroxidase and Phosphatase Stabilizer Reagents also offer excellent performance.

Blocking Reagent NB-2025 (25x)
Blocking Reagent NB-3025 (25x)
Blocking Reagent NB-4025(25x)
250 ml,   (each)

Perox. Stabilizer ST2010 (25x)
Phosph. Stabilizer ST3010 (25x)
Phosph. Stabilizer ST4010 (25x)
100 ml,  (each)


Stability to detergent: Blocks better than casein in presence of Tween.


% recovery of peroxidase activity following dilution in ST2010 (0.1mg/ml final peroxidase concentration).

Much more than just a box.

Multipurpose Incubation Chamber
Ideal for any slide-based application requiring humidified environment. 

A favorite in  Japan. Weighty acrylic construction. Close fitting lid with wide rubber gasket prevents evaporation. Removable glass rods elevate samples. Absorbent black bottom pad for moist keeping and high-contrast viewing. Available in cool grey, and dark orange for UV sensitive applications.

Incubation Chamber, small for 10 slides.
Incubation Chamber, large for 20 slides.


When Only The Best Gels Will Do

Heat Sealable Bags

Multigel® II Mini, Precast PAGE Gels A true favorite in Japan,  available worldwide only from Cosmo Bio. Multigel II’s improved Laemmli formulation and new advanced comb design produce sharp, high resolution bands from top to bottom. Available in fixed percentage, wide-gradient, and narrow gradient formats. A great choice for general research or commercial quality control.  

Multigel II Mini plate size:
100 x 100 x 3.1 mm

Multigel II Mini gel Size:
85 x 90 x 0.9 mm

Certain formulations also available in larger format



4-20% Multigel II Mini Precast PAGE Gel showing Fermentas SM0671 (odd lanes) and SM1811 protein ladders (even lanes).



Choose from Soft, Hard,
or Hard Grid format.

Hybri-Bags ideal for hybridization of membrane blots.

New to Cosmo Bio, Hybri-Bags are easy to seal, strong, tolerate -80C to +80C, powder-free, and reasonably priced. A
vailable with polyethylene (soft) or polypropylene (hard) interiors.  Hard Hybri-bags are autoclavable.

SE-S-1002-EX
  Hybri-Bag, Hard (1cm Grid)
SE-S-1001-EX
  Hybri-Bag, Hard
SE-S-1021-EX
  Hybri-Bag, Soft


Contacts
In the USA, contact Cosmo Bio USA (Carlsbad,CA), info@cosmobiousa.com, 1-866-921-3686.
For all other countries, contact Cosmo Bio Co., Ltd
. (Tokyo),export@cosmobio.co.jp , +(81)-3-5632-9617).

About Cosmo Bio
 
Cosmo Bio brings you select reagents and small instruments for life science research from Japan. With well over 1,000 products from more than 40 Japanese manufacturers and academic labs, the Cosmo Bio catalog conveniently consolidates a wide range of unique and routine research tools. Our catalog includes a large number of antibodies, ELISA kits, and other products based on immunodetection. But also a lot more. Many products from Cosmo Bio have previously been unavailable commercially or outside of Japan. 
 
For over 25 years, Cosmo Bio has been fully committed to exceeding customer expectations by providing......quality products...and the highest standards of customer care and support. 
Orders and Inquiries
Except in the USA, orders ship directly from Japan. USA customers: please contact
Cosmo Bio USA   in Carlsbad, CA (San Diego County). 
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