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| For proteins and nucleic acids. Even staining, high sensitivity, high contrast, low background |
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Background
For detection of proteins and nucleic acids migration by polyacrylamide gel electrophoresis, silver staining is now being highlighted as a sensitive staining method. However the original silver staining method is not necessarily easy to work and takes long-time. 2D-SILVER STAIN-II is an improved reagent kit developed to provide simple and fast staining.
Features
- Rapid staining results in a short time after electrophoresis.
- Staining with higher sensitivity For proteins:50 to 100 times more sensitive than CBB staining For nucleic acids: 50 to 100 times more sensitive than EB staining
- Simple preparation and simple operation.
- No use of such materials as heavy metals controlled by regulations.
- Capable of restaining CBB-stained gels. (Double-staining)
- Staining process can be stopped any time during development for desired chromatic figures.
Applications
2D-SILVER STAINⅡ is applicable to the detection of proteins and nucleic acids subjected to polyacrylamide and SDS-polyacrylamide slab gel electrophoresis.
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Loading buffer： Tris SDS
Sample： PageRuler™ Plus Prestained Protein Ladder, 3 ul each lane (Fermentas UAB #SM1811, 100-200 ug/ul). |
① 1:4 |
⑥ 1:128 |
② 1:8 |
⑦ 1:256 |
③ 1:16 |
⑧ 1:512 |
④ 1:32 |
⑨ 1:1024 |
⑤ 1:64 |
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Components
| Regent |
Contents |
Amt |
| 1) Fixing Reagent |
Thiourea |
100 ml x 1 |
| 2) Pretreatment reagent |
Dithiothreitol, Glutar-aldehyde, Thiourea |
100 ml x 1 |
| 3) Staining Solution A |
Siver nitrate |
100 ml x 1 |
| 4) Staining Solution B |
Ammonium hydroxide, Sodium hydroxide |
100 ml x 1 |
| 5) Concentrated developer |
Citric acid, Folmaldehyde, Sodium hydroxide |
100 ml x 1 |
| 6) Stopper |
Citric acid |
100 ml x 1 |
References
- K. Ohsawa et. Al.: Silver Stain for Detecting 10-Femotogram Quantities of protein after Polyacrylamide Gel Electrophoresis, Anal. Biochem., 135, 409(1983)
- S. Irie: A highly sensitive silver Staining for detection of proteins in polyacrylamide gels, biochemistry(Japan), 52, 411(1980)
- B.R. Oakley et. al.: Visualization of proteins with silver “stain” for detecting proteins in polyacrylamide gels, Anal. Biochem., 105, 361(1980)
- H.M. Poehling et. al.: Visualization of proteins with silver ”stain”, a critical analysis, electrophoresis, 2, 141 (1981)
- U.K. Laemmli: Cleavage of structural proteins during the assembly of the head of bacteriophage T4., Nature, 227, 680(1970)
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